| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print February 21, 2001
Turku Centre for Biotechnology, University of Turku/Åbo Akademi University, Turku 20521
Corresponding Author: markku.salmivirta{at}btk.utu.fi
Fibroblast growth factors (FGFs) are heparin-binding polypeptides that affect the growth, differentiation and migration of many cell types. FGFs signal by binding and activating cell surface FGF receptors (FGFRs) with intracellular tyrosine kinase domains. The signaling involves ligand-induced receptor dimerization and autophosphorylation, followed by downstream transfer of the signal. The sulfated glycosaminoglycans heparin and heparan sulfate bind both FGFs and FGFRs and enhance FGF signaling by mediating complex formation between the growth factor and receptor components. Whereas the heparin/heparan sulfate structures involved in FGF binding have been studied in some detail, little information has been available on saccharide structures mediating binding to FGFRs. We have performed structural characterization of heparin/heparan sulfate oligosaccharides with affinity toward FGFR4. The binding of heparin oligosaccharides to FGFR4 increased with increasing fragment length, the minimal binding domains being contained within eight monosaccharide units. The FGFR4 binding saccharide domains contained both 2-O-sulfated iduronic acid and 6-O-sulfated N-sulfoglucosamine residues, as shown by experiments with selectively desulfated heparin, compositional disaccharide analysis and a novel exoenzyme-based sequence analysis of heparan sulfate oligosaccharides. Structurally distinct heparan sulfate octasaccharides differed in binding to FGFR4. Sequence analysis suggested that the affinity of the interaction depended on the number of 6-O-sulfate groups but not on their precise location.
J. Biol. Chem, 10.1074/jbc.M011226200
Submitted on December 13, 2000
Revised on January 22, 2001
Accepted on February 21, 2001
Binding of heparin/heparan sulfate to fibroblast growth factor receptor 4
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  P. R. Wilker, J. R. Sedy, V. Grigura, T. L. Murphy, and K. M. Murphy Evidence for carbohydrate recognition and homotypic and heterotypic binding by the TIM family Int. Immunol., June 1, 2007; 19(6): 763 - 773. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Jastrebova, M. Vanwildemeersch, A. C. Rapraeger, G. Gimenez-Gallego, U. Lindahl, and D. Spillmann Heparan Sulfate-related Oligosaccharides in Ternary Complex Formation with Fibroblast Growth Factors 1 and 2 and Their Receptors J. Biol. Chem., September 15, 2006; 281(37): 26884 - 26892. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Luo, S. Ye, M. Kan, and W. L. McKeehan Control of Fibroblast Growth Factor (FGF) 7- and FGF1-induced Mitogenesis and Downstream Signaling by Distinct Heparin Octasaccharide Motifs J. Biol. Chem., July 28, 2006; 281(30): 21052 - 21061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Bischof, S. F. Elsawa, G. Mantchev, J. Yoon, G. E. Michels, A. Nilson, S. L. Sutor, J. L. Platt, S. M. Ansell, G. von Bulow, et al. Selective activation of TACI by syndecan-2 Blood, April 15, 2006; 107(8): 3235 - 3242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Saha and D. V. Schaffer Signal dynamics in Sonic hedgehog tissue patterning Development, March 1, 2006; 133(5): 889 - 900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. B. t. Dam, S. Kurup, E. M. A. van de Westerlo, E. M. M. Versteeg, U. Lindahl, D. Spillmann, and T. H. van Kuppevelt 3-O-Sulfated Oligosaccharide Structures Are Recognized by Anti-heparan Sulfate Antibody HS4C3 J. Biol. Chem., February 24, 2006; 281(8): 4654 - 4662. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Robinson, N. J. Harmer, S. J. Goodger, T. L. Blundell, and J. T. Gallagher Cooperative Dimerization of Fibroblast Growth Factor 1 (FGF1) upon a Single Heparin Saccharide May Drive the Formation of 2:2:1 FGF1{middle dot}FGFR2c{middle dot}Heparin Ternary Complexes J. Biol. Chem., December 23, 2005; 280(51): 42274 - 42282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. van den Born, K. Salmivirta, T. Henttinen, N. Ostman, T. Ishimaru, S. Miyaura, K. Yoshida, and M. Salmivirta Novel Heparan Sulfate Structures Revealed by Monoclonal Antibodies J. Biol. Chem., May 27, 2005; 280(21): 20516 - 20523. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bjornson, J. Moses, A. Ingemansson, B. Haraldsson, and J. Sorensson Primary human glomerular endothelial cells produce proteoglycans, and puromycin affects their posttranslational modification Am J Physiol Renal Physiol, April 1, 2005; 288(4): F748 - F756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Dityatev, G. Dityateva, V. Sytnyk, M. Delling, N. Toni, I. Nikonenko, D. Muller, and M. Schachner Polysialylated Neural Cell Adhesion Molecule Promotes Remodeling and Formation of Hippocampal Synapses J. Neurosci., October 20, 2004; 24(42): 9372 - 9382. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. K. Powell, E. A. Yates, D. G. Fernig, and J. E. Turnbull Interactions of heparin/heparan sulfate with proteins: Appraisal of structural factors and experimental approaches Glycobiology, April 1, 2004; 14(4): 17R - 30R. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Borgenstrom, M. Jalkanen, and M. Salmivirta Sulfated Derivatives of Escherichia coli K5 Polysaccharides as Modulators of Fibroblast Growth Factor Signaling J. Biol. Chem., December 12, 2003; 278(50): 49882 - 49889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. L. Wu, L. Zhang, T. Yabe, B. Kuberan, D. L. Beeler, A. Love, and R. D. Rosenberg The Involvement of Heparan Sulfate (HS) in FGF1/HS/FGFR1 Signaling Complex J. Biol. Chem., May 2, 2003; 278(19): 17121 - 17129. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Strott Sulfonation and Molecular Action Endocr. Rev., October 1, 2002; 23(5): 703 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Jemth, J. Kreuger, M. Kusche-Gullberg, L. Sturiale, G. Gimenez-Gallego, and U. Lindahl Biosynthetic Oligosaccharide Libraries for Identification of Protein-binding Heparan Sulfate Motifs. EXPLORING THE STRUCTURAL DIVERSITY BY SCREENING FOR FIBROBLAST GROWTH FACTOR (FGF) 1 AND FGF2 BINDING J. Biol. Chem., August 16, 2002; 277(34): 30567 - 30573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. K. Powell, D. G. Fernig, and J. E. Turnbull Fibroblast Growth Factor Receptors 1 and 2 Interact Differently with Heparin/Heparan Sulfate. IMPLICATIONS FOR DYNAMIC ASSEMBLY OF A TERNARY SIGNALING COMPLEX J. Biol. Chem., August 2, 2002; 277(32): 28554 - 28563. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rolny, D. Spillmann, U. Lindahl, and L. Claesson-Welsh Heparin Amplifies Platelet-derived Growth Factor (PDGF)- BB-induced PDGF alpha -Receptor but Not PDGF beta -Receptor Tyrosine Phosphorylation in Heparan Sulfate-deficient Cells. EFFECTS ON SIGNAL TRANSDUCTION AND BIOLOGICAL RESPONSES J. Biol. Chem., May 24, 2002; 277(22): 19315 - 19321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kreuger, M. Salmivirta, L. Sturiale, G. Gimenez-Gallego, and U. Lindahl Sequence Analysis of Heparan Sulfate Epitopes with Graded Affinities for Fibroblast Growth Factors 1 and 2 J. Biol. Chem., August 10, 2001; 276(33): 30744 - 30752. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
