JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on August 31, 2001
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
276/36/33875    most recent
M011553200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ruponen, M.
Right arrow Articles by Urtti, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruponen, M.
Right arrow Articles by Urtti, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print June 4, 2001
J. Biol. Chem, 10.1074/jbc.M011553200
Submitted on December 21, 2000
Revised on June 4, 2001
Accepted on June 4, 2001

Extracellular glycosaminoglycans modify cellular Trafficking of lipoplexes and polyplexes

Marika Ruponen, Seppo Rönkkö, Paavo Honkakoski, Jukka Pelkonen, Markku Tammi, and Arto Urtti

Department of Pharmaceutics, University of Kuopio, Kuopio 70211 Kuopio

Corresponding Author: Marika.Ruponen{at}uku.fi

Summary It has been shown that extracellular glycosaminoglycans (GAGs) limit the gene transfer by cationic lipids and polymers. The purpose of this study was to clarify how interactions with anionic GAGs (hyaluronic acid and heparan sulfate) modify the cellular uptake and distribution of lipoplexes and polyplexes. Experiments on cellular DNA uptake and GFP reporter gene expression showed that decreased gene expression can rarely be explained by lower cellular uptake. In most cases, the cellular uptake is not changed by GAG binding to the lipoplexes or polyplexes. Reporter gene expression is decreased or blocked by heparan sulfate, but increased by hyaluronic acid. This suggests that intracellular factors are involved. Confocal microscopy experiments demonstrated that extracellular heparan sulfate and hyaluronic acid are taken into cells both with free and DNA-associated carriers. We conclude that extracellular GAGs may alter both the cellular uptake and the intracellular behavior of the DNA complexes.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 IOVSHome page
M. de la Fuente, B. Seijo, and M. J. Alonso
Novel Hyaluronic Acid-Chitosan Nanoparticles for Ocular Gene Therapy
Invest. Ophthalmol. Vis. Sci., May 1, 2008; 49(5): 2016 - 2024.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
L. Peeters, N. N. Sanders, K. Braeckmans, K. Boussery, J. Van de Voorde, S. C. De Smedt, and J. Demeester
Vitreous: A Barrier to Nonviral Ocular Gene Therapy
Invest. Ophthalmol. Vis. Sci., October 1, 2005; 46(10): 3553 - 3561.
[Abstract] [Full Text] [PDF]

Home page
 Journal of Bioactive and Compatible PolymersHome page
M. Boustta, L. Leclercq, and M. Vert
Affinity Chromatography as a Tool to Analyze Polyanion-Polycation Complexes: the Case of Poly(Llysine Citramide)-Poly(L-lysine) Systems
Journal of Bioactive and Compatible Polymers, May 1, 2004; 19(3): 155 - 171.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.