| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print March 16, 2001
Pharmacological Sciences, SUNY at Stony Brook, Stony Brook, NY 11794-8651
Corresponding Author: maki{at}pharm.sunysb.edu
1,N6-Ethenodeoxyadenosine, a DNA adduct generated by exogenous and endogenous sources, severely blocks DNA synthesis and induces miscoding events in human cells. To probe the mechanism for in vivo translesion DNA synthesis across this adduct, in vitro primer extension studies were conducted using newly identified human DNA polymerases
J. Biol. Chem, 10.1074/jbc.M102158200
Submitted on March 9, 2001
Revised on March 16, 2001
Accepted on March 15, 2001
Translesion DNA synthesis catalyzed by human pol
and pol 
across 1,N6-ethenodeoxyadenosine
and 
, which have been shown to catalyze translesion DNA synthesis past several DNA lesions. Steady-state kinetic analyses and analysis of translesion products have revealed that the synthesis is >100 fold more efficient with pol than with pol and that both error-free and error-prone syntheses are observed with these enzymes. The miscoding events include both base substitution and frameshift mutations. These results suggest that both polymerases, particularly pol , may contribute to the TLS events observed for edA in human cells.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. H. Tolentino, T. J. Burke, S. Mukhopadhyay, W. G. McGregor, and A. K. Basu Inhibition of DNA replication fork progression and mutagenic potential of 1, N6-ethenoadenine and 8-oxoguanine in human cell extracts Nucleic Acids Res., March 27, 2008; 36(4): 1300 - 1308. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Johnson, L. Haracska, L. Prakash, and S. Prakash Role of Hoogsteen Edge Hydrogen Bonding at Template Purines in Nucleotide Incorporation by Human DNA Polymerase {iota} Mol. Cell. Biol., September 1, 2006; 26(17): 6435 - 6441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Choi, K. C. Angel, and F. P. Guengerich Translesion Synthesis across Bulky N2-Alkyl Guanine DNA Adducts by Human DNA Polymerase {kappa} J. Biol. Chem., July 28, 2006; 281(30): 21062 - 21072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. Wolfle, M. T. Washington, E. T. Kool, T. E. Spratt, S. A. Helquist, L. Prakash, and S. Prakash Evidence for a Watson-Crick Hydrogen Bonding Requirement in DNA Synthesis by Human DNA Polymerase {kappa} Mol. Cell. Biol., August 15, 2005; 25(16): 7137 - 7143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Jaloszynski, E. Ohashi, H. Ohmori, and S. Nishimura Error-prone and inefficient replication across 8-hydroxyguanine (8-oxoguanine) in human and mouse ras gene fragments by DNA polymerase {kappa} Genes Cells, June 1, 2005; 10(6): 543 - 550. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Jaloszynski, C. Masutani, F. Hanaoka, A. B. Perez, and S. Nishimura 8-Hydroxyguanine in a mutational hotspot of the c-Ha-ras gene causes misreplication, 'action-at-a-distance' mutagenesis and inhibition of replication Nucleic Acids Res., November 1, 2003; 31(21): 6085 - 6095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Washington, W. T. Wolfle, T. E. Spratt, L. Prakash, and S. Prakash Yeast DNA polymerase eta makes functional contacts with the DNA minor groove only at the incoming nucleoside triphosphate PNAS, April 29, 2003; 100(9): 5113 - 5118. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I.-Y. Yang, H. Miller, Z. Wang, E. G. Frank, H. Ohmori, F. Hanaoka, and M. Moriya Mammalian Translesion DNA Synthesis across an Acrolein-derived Deoxyguanosine Adduct. PARTICIPATION OF DNA POLYMERASE eta IN ERROR-PRONE SYNTHESIS IN HUMAN CELLS J. Biol. Chem., April 11, 2003; 278(16): 13989 - 13994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. G. Minko, M. T. Washington, M. Kanuri, L. Prakash, S. Prakash, and R. S. Lloyd Translesion Synthesis past Acrolein-derived DNA Adduct, gamma -Hydroxypropanodeoxyguanosine, by Yeast and Human DNA Polymerase eta J. Biol. Chem., January 3, 2003; 278(2): 784 - 790. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Haracska, L. Prakash, and S. Prakash Role of human DNA polymerase kappa as an extender in translesion synthesis PNAS, December 10, 2002; 99(25): 16000 - 16005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Okada, E. Sonoda, Y. M. Yamashita, S. Koyoshi, S. Tateishi, M. Yamaizumi, M. Takata, O. Ogawa, and S. Takeda Involvement of Vertebrate Polkappa in Rad18-independent Postreplication Repair of UV Damage J. Biol. Chem., December 6, 2002; 277(50): 48690 - 48695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ogi, Y. Shinkai, K. Tanaka, and H. Ohmori Polkappa protects mammalian cells against the lethal and mutagenic effects of benzo[a]pyrene PNAS, November 26, 2002; 99(24): 15548 - 15553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. L. Fischhaber, V. L. Gerlach, W. J. Feaver, Z. Hatahet, S. S. Wallace, and E. C. Friedberg Human DNA Polymerase kappa Bypasses and Extends beyond Thymine Glycols during Translesion Synthesis in Vitro, Preferentially Incorporating Correct Nucleotides J. Biol. Chem., September 27, 2002; 277(40): 37604 - 37611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Rechkoblit, Y. Zhang, D. Guo, Z. Wang, S. Amin, J. Krzeminsky, N. Louneva, and N. E. Geacintov trans-Lesion Synthesis Past Bulky Benzo[a]pyrene Diol Epoxide N2-dG and N6-dA Lesions Catalyzed by DNA Bypass Polymerases J. Biol. Chem., August 16, 2002; 277(34): 30488 - 30494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Prakash and L. Prakash Translesion DNA synthesis in eukaryotes: A one- or two-polymerase affair Genes & Dev., August 1, 2002; 16(15): 1872 - 1883. [Full Text] [PDF]
|
|
|
|
|
|
R. J. Kokoska, K. Bebenek, F. Boudsocq, R. Woodgate, and T. A. Kunkel Low Fidelity DNA Synthesis by a Y Family DNA Polymerase Due to Misalignment in the Active Site J. Biol. Chem., May 24, 2002; 277(22): 19633 - 19638. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanuri, I. G. Minko, L. V. Nechev, T. M. Harris, C. M. Harris, and R. S. Lloyd Error Prone Translesion Synthesis Past gamma -Hydroxypropano Deoxyguanosine, the Primary Acrolein-derived Adduct in Mammalian Cells J. Biol. Chem., May 17, 2002; 277(21): 18257 - 18265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhang, X. Wu, O. Rechkoblit, N. E. Geacintov, J.-S. Taylor, and Z. Wang Response of human REV1 to different DNA damage: preferential dCMP insertion opposite the lesion Nucleic Acids Res., April 1, 2002; 30(7): 1630 - 1638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bergoglio, C. Bavoux, V. Verbiest, J.-S. Hoffmann, and C. Cazaux Localisation of human DNA polymerase {kappa} to replication foci J. Cell Sci., January 12, 2002; 115(23): 4413 - 4418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Wang DNA DAMAGE-INDUCED MUTAGENESIS : A NOVEL TARGET FOR CANCER PREVENTION Mol. Interv., December 1, 2001; 1(5): 269 - 281. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
