| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print June 19, 2001
J. Biol. Chem, 10.1074/jbc.M102254200
Submitted on March 13, 2001
Revised on June 13, 2001
Accepted on June 18, 2001
Internal Medicine and Biochemistry, UT-Southwestern Medical Center, Dallas, TX 75390-8573
Corresponding Author: thomas.kodadek{at}utsouthwestern.edu
An in vivo protein interaction assay was used to search a yeast cDNA library for proteins that bind to the acidic activation domain (AD) of the yeast Gal4 protein. Sug2 protein, a component of the 19S regulatory particle of the 26S proteasome, was one of seven proteins identified in this screen. In vitro binding assays confirm a direct interaction between these proteins. SUG2 and SUG1, another 19S component, were originally discovered as a mutation able to suppress the phenotype of a Gal4 truncation mutant (Gal4Dp) lacking much of its AD. Sug1p has previously been shown to bind the Gal4 AD in vitro. Taken together, these genetic and biochemical data suggest a biologically significant interaction between the Gal4 protein and the 19S regulatory particle of the proteasome. Indeed, it is demonstrated here that the Gal4 AD interacts specifically with immunopurified 19S complex. The proteasome regulatory particle has recently been shown to play a direct role in RNA polymerase II transcription and the activator-19S interaction could be important in recruiting this large complex to transcriptionally active GAL genes.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C. T. Archer, L. Burdine, B. Liu, A. Ferdous, S. A. Johnston, and T. Kodadek Physical and Functional Interactions of Monoubiquitylated Transactivators with the Proteasome J. Biol. Chem., August 1, 2008; 283(31): 21789 - 21798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Kasahara, S. Ki, K. Aoyama, H. Takahashi, and T. Kokubo Saccharomyces cerevisiae HMO1 interacts with TFIID and participates in start site selection by RNA polymerase II Nucleic Acids Res., March 27, 2008; 36(4): 1343 - 1357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Ferdous, D. Sikder, T. Gillette, K. Nalley, T. Kodadek, and S. A. Johnston The role of the proteasomal ATPases and activator monoubiquitylation in regulating Gal4 binding to promoters Genes & Dev., January 1, 2007; 21(1): 112 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-A. Bruun, E. I. S. Thomassen, K. Kristiansen, G. Tylden, T. Holm, I. Mikkola, G. Bjorkoy, and T. Johansen The third helix of the homeodomain of paired class homeodomain proteins acts as a recognition helix both for DNA and protein interactions Nucleic Acids Res., May 10, 2005; 33(8): 2661 - 2675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H K Kinyamu, J Chen, and T K Archer Linking the ubiquitin-proteasome pathway to chromatin remodeling/modification by nuclear receptors J. Mol. Endocrinol., April 1, 2005; 34(2): 281 - 297. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Moraitis and V. Giguere The Co-repressor Hairless Protects ROR{alpha} Orphan Nuclear Receptor from Proteasome-mediated Degradation J. Biol. Chem., December 26, 2003; 278(52): 52511 - 52518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. Kohlhaw Leucine Biosynthesis in Fungi: Entering Metabolism through the Back Door Microbiol. Mol. Biol. Rev., March 1, 2003; 67(1): 1 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Klein, M. Nolden, S. L. Sanders, J. Kirchner, P. A. Weil, and K. Melcher Use of a Genetically Introduced Cross-linker to Identify Interaction Sites of Acidic Activators within Native Transcription Factor IID and SAGA J. Biol. Chem., February 21, 2003; 278(9): 6779 - 6786. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
