JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on June 29, 2001
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
276/27/24531    most recent
M102678200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gineitis, D.
Right arrow Articles by Treisman, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gineitis, D.
Right arrow Articles by Treisman, R.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print May 7, 2001
J. Biol. Chem, 10.1074/jbc.M102678200
Submitted on March 26, 2001
Revised on April 27, 2001
Accepted on May 6, 2001

Differential usage of signal transduction pathways defines two types of SRF target gene

Dziugas Gineitis and Richard Treisman

Transcription Laboratory, Imperial Cancer Research Fund Laboratories, London WC2A 3PX

Corresponding Author: R.Treisman{at}icrf.icnet.uk

Activation of the transcription factor SRF is dependent on Rho-controlled changes in actin dynamics. We used pathway-specific inhibitors to compare the roles of actin dynamics, MEK-ERK signalling, and Phosphatidylinositol 3-kinase (PI-3K) in signalling either to SRF itself or to four cellular SRF target genes. Serum, LPA, PDGF and the phorbol ester TPA each activated transcription of a stably integrated SRF reporter gene, dependent on functional RhoA GTPase. Inhibition of MEK reduced activation of the SRF reporter by all stimuli by about 50%, except for TPA, which was effectively blocked. Inhibition of PI-3K slightly reduced reporter activation by serum and LPA, but substantially inhibited activation by PDGF and TPA. Reporter induction by all stimuli was absolutely dependent on actin dynamics. Regulation of the SRF (srf) and vinculin (vcl) genes was similar to that of the SRF reporter gene: activation by all stimuli was Rho-dependent and required actin dynamics, but was largely independent of MEK activity. In contrast, activation of fos and egr1 occured independently of RhoA and actin polymerisation, but was almost completely dependent on MEK activation. These results show that at least two classes of SRF target genes can be distinguished on the basis of their relative sensitivity to RhoA-actin and MEK-ERK signalling pathways.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
S. Muehlich, R. Wang, S.-M. Lee, T. C. Lewis, C. Dai, and R. Prywes
Serum-Induced Phosphorylation of the Serum Response Factor Coactivator MKL1 by the Extracellular Signal-Regulated Kinase 1/2 Pathway Inhibits Its Nuclear Localization
Mol. Cell. Biol., October 15, 2008; 28(20): 6302 - 6313.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Hoffmann, J. Ashouri, S. Wolter, A. Doerrie, O. Dittrich-Breiholz, H. Schneider, E. F. Wagner, J. Troppmair, N. Mackman, and M. Kracht
Transcriptional Regulation of EGR-1 by the Interleukin-1-JNK-MKK7-c-Jun Pathway
J. Biol. Chem., May 2, 2008; 283(18): 12120 - 12128.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Busche, A. Descot, S. Julien, H. Genth, and G. Posern
Epithelial cell-cell contacts regulate SRF-mediated transcription via Rac-actin-MAL signalling
J. Cell Sci., April 1, 2008; 121(7): 1025 - 1035.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
X. Hou, E. W. Arvisais, C. Jiang, D.-b. Chen, S. K. Roy, J. L. Pate, T. R. Hansen, B. R. Rueda, and J. S. Davis
Prostaglandin F2{alpha} Stimulates the Expression and Secretion of Transforming Growth Factor B1 Via Induction of the Early Growth Response 1 Gene (EGR1) in the Bovine Corpus Luteum
Mol. Endocrinol., February 1, 2008; 22(2): 403 - 414.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. Y. Winter, T. Dayaram, and S. J. Marriott
Activation of the Human T-Cell Leukemia Virus Type 1 Long Terminal Repeat by the Ternary Complex Factor Elk-1
J. Virol., December 1, 2007; 81(23): 13075 - 13081.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. D. Gopinath, S. Narumiya, and J. Dhawan
The RhoA effector mDiaphanous regulates MyoD expression and cell cycle progression via SRF-dependent and SRF-independent pathways
J. Cell Sci., September 1, 2007; 120(17): 3086 - 3098.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. W. Tullai, M. E. Schaffer, S. Mullenbrock, G. Sholder, S. Kasif, and G. M. Cooper
Immediate-Early and Delayed Primary Response Genes Are Distinct in Function and Genomic Architecture
J. Biol. Chem., August 17, 2007; 282(33): 23981 - 23995.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. S. Parmacek
Myocardin-Related Transcription Factors: Critical Coactivators Regulating Cardiovascular Development and Adaptation
Circ. Res., March 16, 2007; 100(5): 633 - 644.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
D. P. Staus, A. L. Blaker, J. M. Taylor, and C. P. Mack
Diaphanous 1 and 2 Regulate Smooth Muscle Cell Differentiation by Activating the Myocardin-Related Transcription Factors
Arterioscler. Thromb. Vasc. Biol., March 1, 2007; 27(3): 478 - 486.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. M. Miano, X. Long, and K. Fujiwara
Serum response factor: master regulator of the actin cytoskeleton and contractile apparatus
Am J Physiol Cell Physiol, January 1, 2007; 292(1): C70 - C81.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Sun, K. Boyd, W. Xu, J. Ma, C. W. Jackson, A. Fu, J. M. Shillingford, G. W. Robinson, L. Hennighausen, J. K. Hitzler, et al.
Acute Myeloid Leukemia-Associated Mkl1 (Mrtf-a) Is a Key Regulator of Mammary Gland Function
Mol. Cell. Biol., August 1, 2006; 26(15): 5809 - 5826.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
A.-I. Zaromytidou, F. Miralles, and R. Treisman
MAL and Ternary Complex Factor Use Different Mechanisms To Contact a Common Surface on the Serum Response Factor DNA-Binding Domain.
Mol. Cell. Biol., June 1, 2006; 26(11): 4134 - 4148.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. H. Brown, D. P. Del Re, and M. A. Sussman
The Rac and Rho Hall of Fame: A Decade of Hypertrophic Signaling Hits
Circ. Res., March 31, 2006; 98(6): 730 - 742.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. Iyer, D. Chang, J. Marx, L. Wei, E. N. Olson, M. S. Parmacek, A. Balasubramanyam, and R. J. Schwartz
Serum response factor MADS box serine -162 phosphorylation switches proliferation and myogenic gene programs
PNAS, March 21, 2006; 103(12): 4516 - 4521.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
M. Zhao, R. G. DiScipio, A. G. Wimmer, and I. U. Schraufstatter
Regulation of CXCR4-Mediated Nuclear Translocation of Extracellular Signal-Related Kinases 1 and 2
Mol. Pharmacol., January 1, 2006; 69(1): 66 - 75.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Kumbrink, M. Gerlinger, and J. P. Johnson
Egr-1 Induces the Expression of Its Corepressor Nab2 by Activation of the Nab2 Promoter Thereby Establishing a Negative Feedback Loop
J. Biol. Chem., December 30, 2005; 280(52): 42785 - 42793.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Rene, M. Taulan, F. Iral, J. Doudement, A. L'Honore, C. Gerbon, J. Demaille, M. Claustres, and M.-C. Romey
Binding of serum response factor to cystic fibrosis transmembrane conductance regulator CArG-like elements, as a new potential CFTR transcriptional regulation pathway
Nucleic Acids Res., September 16, 2005; 33(16): 5271 - 5290.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. N. McLaughlin, M. R. Mazzoni, J. H. Cleator, L. Earls, A. L. Perdigoto, J. D. Brooks, J. A. S. Muldowney III, D. E. Vaughan, and H. E. Hamm
Thrombin Modulates the Expression of a Set of Genes Including Thrombospondin-1 in Human Microvascular Endothelial Cells
J. Biol. Chem., June 10, 2005; 280(23): 22172 - 22180.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Alberti, S. M. Krause, O. Kretz, U. Philippar, T. Lemberger, E. Casanova, F. F. Wiebel, H. Schwarz, M. Frotscher, G. Schutz, et al.
Neuronal migration in the murine rostral migratory stream requires serum response factor
PNAS, April 26, 2005; 102(17): 6148 - 6153.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Kuwahara, T. Barrientos, G. C. T. Pipes, S. Li, and E. N. Olson
Muscle-Specific Signaling Mechanism That Links Actin Dynamics to Serum Response Factor
Mol. Cell. Biol., April 15, 2005; 25(8): 3173 - 3181.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. van Riggelen, G. Buchwalter, U. Soto, J. De-Castro Arce, H. z. Hausen, B. Wasylyk, and F. Rosl
Loss of Net as Repressor Leads to Constitutive Increased c-fos Transcription in Cervical Cancer Cells
J. Biol. Chem., February 4, 2005; 280(5): 3286 - 3294.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Kasza, A. O'Donnell, K. Gascoigne, L. A. H. Zeef, A. Hayes, and A. D. Sharrocks
The ETS Domain Transcription Factor Elk-1 Regulates the Expression of Its Partner Protein, SRF
J. Biol. Chem., January 14, 2005; 280(2): 1149 - 1155.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
X. Zhe, Y. Yang, and L. Schuger
Imbalanced Plasminogen System in Lymphangioleiomyomatosis: Potential Role of Serum Response Factor
Am. J. Respir. Cell Mol. Biol., January 1, 2005; 32(1): 28 - 34.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Zhang, G. Azhar, Y. Zhong, and J. Y. Wei
Identification of a Novel Serum Response Factor Cofactor in Cardiac Gene Regulation
J. Biol. Chem., December 31, 2004; 279(53): 55626 - 55632.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
E. R. Vickers, A. Kasza, I. A. Kurnaz, A. Seifert, L. A. H. Zeef, A. O'Donnell, A. Hayes, and A. D. Sharrocks
Ternary Complex Factor-Serum Response Factor Complex-Regulated Gene Activity Is Required for Cellular Proliferation and Inhibition of Apoptotic Cell Death
Mol. Cell. Biol., December 1, 2004; 24(23): 10340 - 10351.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
B.R. Wamhoff, M.H. Hoofnagle, A. Burns, S. Sinha, O.G. McDonald, and G.K. Owens
A G/C Element Mediates Repression of the SM22{alpha} Promoter Within Phenotypically Modulated Smooth Muscle Cells in Experimental Atherosclerosis
Circ. Res., November 12, 2004; 95(10): 981 - 988.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Lockman, J. S. Hinson, M. D. Medlin, D. Morris, J. M. Taylor, and C. P. Mack
Sphingosine 1-Phosphate Stimulates Smooth Muscle Cell Differentiation and Proliferation by Activating Separate Serum Response Factor Co-factors
J. Biol. Chem., October 8, 2004; 279(41): 42422 - 42430.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. W. Tullai, M. E. Schaffer, S. Mullenbrock, S. Kasif, and G. M. Cooper
Identification of Transcription Factor Binding Sites Upstream of Human Genes Regulated by the Phosphatidylinositol 3-Kinase and MEK/ERK Signaling Pathways
J. Biol. Chem., May 7, 2004; 279(19): 20167 - 20177.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Zhuang, G. T. Nguyen, Y. Chen, T. Gudi, M. Eigenthaler, T. Jarchau, U. Walter, G. R. Boss, and R. B. Pilz
Vasodilator-stimulated Phosphoprotein Activation of Serum-response Element-dependent Transcription Occurs Downstream of RhoA and Is Inhibited by cGMP-dependent Protein Kinase Phosphorylation
J. Biol. Chem., March 12, 2004; 279(11): 10397 - 10407.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. I. Reinhold, D. G. McEwen, and M. C. Naski
Fibroblast Growth Factor Receptor 3 Gene: Regulation by Serum Response Factor
Mol. Endocrinol., January 1, 2004; 18(1): 241 - 251.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Ott, D. Iwanciw, A. Graness, K. Giehl, and M. Goppelt-Struebe
Modulation of the Expression of Connective Tissue Growth Factor by Alterations of the Cytoskeleton
J. Biol. Chem., November 7, 2003; 278(45): 44305 - 44311.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
R. E. Ward, J. Evans, and C. S. Thummel
Genetic Modifier Screens in Drosophila Demonstrate a Role for Rho1 Signaling in Ecdysone-Triggered Imaginal Disc Morphogenesis
Genetics, November 1, 2003; 165(3): 1397 - 1415.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Cen, A. Selvaraj, R. C. Burgess, J. K. Hitzler, Z. Ma, S. W. Morris, and R. Prywes
Megakaryoblastic Leukemia 1, a Potent Transcriptional Coactivator for Serum Response Factor (SRF), Is Required for Serum Induction of SRF Target Genes
Mol. Cell. Biol., September 15, 2003; 23(18): 6597 - 6608.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
A. Zeidan, I. Nordstrom, S. Albinsson, U. Malmqvist, K. Sward, and P. Hellstrand
Stretch-induced contractile differentiation of vascular smooth muscle: sensitivity to actin polymerization inhibitors
Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1387 - C1396.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Percipalle, N. Fomproix, K. Kylberg, F. Miralles, B. Bjorkroth, B. Daneholt, and N. Visa
An actin-ribonucleoprotein interaction is involved in transcription by RNA polymerase II
PNAS, May 27, 2003; 100(11): 6475 - 6480.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Huang, M. Paliouras, I. Rambaldi, P. Lasko, and M. Featherstone
Nonmuscle Myosin Promotes Cytoplasmic Localization of PBX
Mol. Cell. Biol., May 15, 2003; 23(10): 3636 - 3645.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. L'honore, N. J. Lamb, M. Vandromme, P. Turowski, G. Carnac, and A. Fernandez
MyoD Distal Regulatory Region Contains an SRF Binding CArG Element Required for MyoD Expression in Skeletal Myoblasts and during Muscle Regeneration
Mol. Biol. Cell, May 1, 2003; 14(5): 2151 - 2162.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
G. Posern, A. Sotiropoulos, and R. Treisman
Mutant Actins Demonstrate a Role for Unpolymerized Actin in Control of Transcription by Serum Response Factor
Mol. Biol. Cell, December 1, 2002; 13(12): 4167 - 4178.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Lin, D. Wang, and W. Sadee
Serum Response Factor Activation by Muscarinic Receptors via RhoA. NOVEL PATHWAY SPECIFIC TO M1 SUBTYPE INVOLVING CALMODULIN, CALCINEURIN, AND Pyk2
J. Biol. Chem., October 18, 2002; 277(43): 40789 - 40798.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
K. Murai and R. Treisman
Interaction of Serum Response Factor (SRF) with the Elk-1 B Box Inhibits RhoA-Actin Signaling to SRF and Potentiates Transcriptional Activation by Elk-1
Mol. Cell. Biol., October 15, 2002; 22(20): 7083 - 7092.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Gudi, J. C. Chen, D. E. Casteel, T. M. Seasholtz, G. R. Boss, and R. B. Pilz
cGMP-dependent Protein Kinase Inhibits Serum-response Element-dependent Transcription by Inhibiting Rho Activation and Functions
J. Biol. Chem., September 27, 2002; 277(40): 37382 - 37393.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Psichari, A. Balmain, D. Plows, V. Zoumpourlis, and A. Pintzas
High Activity of Serum Response Factor in the Mesenchymal Transition of Epithelial Tumor Cells Is Regulated by RhoA Signaling
J. Biol. Chem., August 9, 2002; 277(33): 29490 - 29495.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
G. J. Inman, F. J. Nicolas, J. F. Callahan, J. D. Harling, L. M. Gaster, A. D. Reith, N. J. Laping, and C. S. Hill
SB-431542 Is a Potent and Specific Inhibitor of Transforming Growth Factor-beta Superfamily Type I Activin Receptor-Like Kinase (ALK) Receptors ALK4, ALK5, and ALK7
Mol. Pharmacol., July 1, 2002; 62(1): 65 - 74.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
O. Geneste, J. W. Copeland, and R. Treisman
LIM kinase and Diaphanous cooperate to regulate serum response factor and actin dynamics
J. Cell Biol., May 28, 2002; 157(5): 831 - 838.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Charvet, P. Auberger, S. Tartare-Deckert, A. Bernard, and M. Deckert
Vav1 Couples T Cell Receptor to Serum Response Factor-dependent Transcription via a MEK-dependent Pathway
J. Biol. Chem., May 3, 2002; 277(18): 15376 - 15384.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
G. R. Wiggin, A. Soloaga, J. M. Foster, V. Murray-Tait, P. Cohen, and J. S. C. Arthur
MSK1 and MSK2 Are Required for the Mitogen- and Stress-Induced Phosphorylation of CREB and ATF1 in Fibroblasts
Mol. Cell. Biol., April 15, 2002; 22(8): 2871 - 2881.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. A. Tice, I. Soloviev, and P. Polakis
Activation of the Wnt Pathway Interferes with Serum Response Element-driven Transcription of Immediate Early Genes
J. Biol. Chem., February 15, 2002; 277(8): 6118 - 6123.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. L. Stevens, G. T. Cantin, G. Wang, A. Shevchenko, A. Shevchenko, and A. J. Berk
Transcription Control by E1A and MAP Kinase Pathway via Sur2 Mediator Subunit
Science, April 26, 2002; 296(5568): 755 - 758.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.