| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print August 27, 2001
Department of Pharmacology, McGill University, Montreal, Quebec H3G 1Y6
Corresponding Author: mszyf{at}pharma.mcgill.ca
Mammalian genomes are compartmentalized into dense inactive chromatin that is hypermethylated and active open chromatin that is hypomethylated. It is generally accepted that this bimodal pattern of methylation is established during development and is then faithfully inherited through subsequent cell divisions by a maintenance DNA methyltransferase (DNMT1) . The pattern of methylation is believed to direct local histone acetylation states. In contrast to this well accepted consensus we show here using a transient transfection model that an active demethylase is involved in shaping patterns of methylation in somatic cells. Demethylase activity is directed by the state of histone acetylation and therefore the resulting methylation pattern is determined by local histone acetylation states contrary to the accepted model. Our data supports a new model suggesting that the pattern of methylation is maintained by a dynamic balance of methylation and demethylation activities and the local state of histone acetylation. This provides a simple mechanism for explaining why active genes are not methylated.
J. Biol. Chem, 10.1074/jbc.M103921200
Submitted on May 1, 2001
Revised on August 27, 2001
Accepted on August 25, 2001
Demethylase activity is directed by histone acetylation
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Vincent, M.-P. Ducourouble, and I. Van Seuningen Epigenetic regulation of the human mucin gene MUC4 in epithelial cancer cell lines involves both DNA methylation and histone modifications mediated by DNA methyltransferases and histone deacetylases FASEB J, August 1, 2008; 22(8): 3035 - 3045. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Minoguchi and H. Iba Instability of Retroviral DNA Methylation in Embryonic Stem Cells Stem Cells, May 1, 2008; 26(5): 1166 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Szyf The Dynamic Epigenome and its Implications in Toxicology Toxicol. Sci., November 1, 2007; 100(1): 7 - 23. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C. D'Alessio, I. C. G. Weaver, and M. Szyf Acetylation-Induced Transcription Is Required for Active DNA Demethylation in Methylation-Silenced Genes Mol. Cell. Biol., November 1, 2007; 27(21): 7462 - 7474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. S Oliveri, M. Kalisz, C. K. Schjerling, C. Y. Andersen, R. Borup, and A. G. Byskov Evaluation in mammalian oocytes of gene transcripts linked to epigenetic reprogramming Reproduction, October 1, 2007; 134(4): 549 - 558. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Brown and M. Szyf Epigenetic Programming of the rRNA Promoter by MBD3 Mol. Cell. Biol., July 1, 2007; 27(13): 4938 - 4952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Milutinovic, A. C. D'Alessio, N. Detich, and M. Szyf Valproate induces widespread epigenetic reprogramming which involves demethylation of specific genes Carcinogenesis, March 1, 2007; 28(3): 560 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. C. G. Weaver, A. C. D'Alessio, S. E. Brown, I. C. Hellstrom, S. Dymov, S. Sharma, M. Szyf, and M. J. Meaney The Transcription Factor Nerve Growth Factor-Inducible Protein A Mediates Epigenetic Programming: Altering Epigenetic Marks by Immediate-Early Genes J. Neurosci., February 14, 2007; 27(7): 1756 - 1768. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rybouchkin, Y. Kato, and Y. Tsunoda Role of Histone Acetylation in Reprogramming of Somatic Nuclei Following Nuclear Transfer Biol Reprod, June 1, 2006; 74(6): 1083 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Kassis, M. Zhao, J. A. Hong, G. A. Chen, D. M. Nguyen, and D. S. Schrump Depletion of DNA methyltransferase 1 and/or DNA methyltransferase 3b mediates growth arrest and apoptosis in lung and esophageal cancer and malignant pleural mesothelioma cells J. Thorac. Cardiovasc. Surg., February 1, 2006; 131(2): 298 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bonilla-Henao, R. Martinez, F. Sobrino, and E. Pintado Different signaling pathways inhibit DNA methylation activity and up-regulate IFN-{gamma} in human lymphocytes J. Leukoc. Biol., December 1, 2005; 78(6): 1339 - 1346. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Tao, W. Wang, L. Li, P. K. Kramer, and M. A. Pereira DNA Hypomethylation Induced by Drinking Water Disinfection By-Products in Mouse and Rat Kidney Toxicol. Sci., October 1, 2005; 87(2): 344 - 352. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Iseki, R. Shimizukawa, F. Sugiyama, S. Kunita, A. Iwama, M. Onodera, H. Nakauchi, and K.-i. Yagami Parvovirus Nonstructural Proteins Induce an Epigenetic Modification through Histone Acetylation in Host Genes and Revert Tumor Malignancy to Benignancy J. Virol., July 15, 2005; 79(14): 8886 - 8893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Gioia, B. Barboni, M. Turriani, G. Capacchietti, M. G. Pistilli, P. Berardinelli, and M. Mattioli The capability of reprogramming the male chromatin after fertilization is dependent on the quality of oocyte maturation Reproduction, July 1, 2005; 130(1): 29 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Xiong, S. C. Dowdy, K. C. Podratz, F. Jin, J. R. Attewell, N. L. Eberhardt, and S.-W. Jiang Histone Deacetylase Inhibitors Decrease DNA Methyltransferase-3B Messenger RNA Stability and Down-regulate De novo DNA Methyltransferase Activity in Human Endometrial Cells Cancer Res., April 1, 2005; 65(7): 2684 - 2689. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Pakneshan, M. Szyf, and S. A. Rabbani Methylation and inhibition of expression of uPA by the RAS oncogene: divergence of growth control and invasion in breast cancer cells Carcinogenesis, March 1, 2005; 26(3): 557 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. M. Campbell, V. Bovenzi, and M. Szyf Methylated DNA-binding protein 2 antisense inhibitors suppress tumourigenesis of human cancer cell lines in vitro and in vivo Carcinogenesis, April 1, 2004; 25(4): 499 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rehtanz, H.-M. Schmidt, U. Warthorst, and G. Steger Direct Interaction between Nucleosome Assembly Protein 1 and the Papillomavirus E2 Proteins Involved in Activation of Transcription Mol. Cell. Biol., March 1, 2004; 24(5): 2153 - 2168. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Detich, V. Bovenzi, and M. Szyf Valproate Induces Replication-independent Active DNA Demethylation J. Biol. Chem., July 18, 2003; 278(30): 27586 - 27592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Sato, A. Maitra, N. Fukushima, N. T. van Heek, H. Matsubayashi, C. A. Iacobuzio-Donahue, C. Rosty, and M. Goggins Frequent Hypomethylation of Multiple Genes Overexpressed in Pancreatic Ductal Adenocarcinoma Cancer Res., July 15, 2003; 63(14): 4158 - 4166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ji, J. Zhang, A. I. Lee, H. Cedar, and Y. Bergman A multistep mechanism for the activation of rearrangement in the immune system PNAS, June 24, 2003; 100(13): 7557 - 7562. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Detich, S. Hamm, G. Just, J. D. Knox, and M. Szyf The Methyl Donor S-Adenosylmethionine Inhibits Active Demethylation of DNA: A CANDIDATE NOVEL MECHANISM FOR THE PHARMACOLOGICAL EFFECTS OF S-ADENOSYLMETHIONINE J. Biol. Chem., May 30, 2003; 278(23): 20812 - 20820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Detich, J. Theberge, and M. Szyf Promoter-specific Activation and Demethylation by MBD2/Demethylase J. Biol. Chem., September 20, 2002; 277(39): 35791 - 35794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Cervoni, N. Detich, S.-b. Seo, D. Chakravarti, and M. Szyf The Oncoprotein Set/TAF-1beta , an Inhibitor of Histone Acetyltransferase, Inhibits Active Demethylation of DNA, Integrating DNA Methylation and Transcriptional Silencing J. Biol. Chem., July 5, 2002; 277(28): 25026 - 25031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Chen, R. P. Sharma, R. H. Costa, E. Costa, and D. R. Grayson On the epigenetic regulation of the human reelin promoter Nucleic Acids Res., July 1, 2002; 30(13): 2930 - 2939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Hays, J. Swanson, and E. U. Selker Identification and Characterization of the Genes Encoding the Core Histones and Histone Variants of Neurospora crassa Genetics, March 1, 2002; 160(3): 961 - 973. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Lee, S. G. Hwang, J. Kim, and J. Choe Functional Interaction between p/CAF and Human Papillomavirus E2 Protein J. Biol. Chem., February 15, 2002; 277(8): 6483 - 6489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Costa, Y. Chen, J. Davis, E. Dong, J.S. Noh, L. Tremolizzo, M. Veldic, D.R. Grayson, and A. Guidotti REELIN and Schizophrenia:: A Disease at the Interface of the Genome and the Epigenome Mol. Interv., February 1, 2002; 2(1): 47 - 57. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
