Binding and repair of mismatched DNA mediated by Rhp14 the fission yeast homologue of human XPA

doi:10.1074/jbc.M104039200

QUICK SEARCH:

	Author:		Keyword(s):
Year:		Vol:		Page:

A more recent version of this article appeared on August 10, 2001

This Article

	Full Text (Accepted Manuscript)
	All Versions of this Article: 276/33/30766 most recent M104039200v1
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Email this article to a friend
	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager


Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Hohl, M.
	Articles by Fleck, O.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Hohl, M.
	Articles by Fleck, O.

Social Bookmarking

	What's this?

Papers In Press, published online ahead of print June 14, 2001
J. Biol. Chem, 10.1074/jbc.M104039200
Submitted on May 4, 2001
Revised on June 8, 2001
Accepted on June 10, 2001

Binding and repair of mismatched DNA mediated by Rhp14 the fission yeast homologue of human XPA

Marcel Hohl, Olaf Christensen, Christophe Kunz, Hanspeter Naegeli, and Oliver Fleck

Institute of Cell Biology, Bern CH-3012

Corresponding Author: fleck{at}izb.unibe.ch

Rhp14 of Schizosaccharomyces pombe is homologous to human XPA and Saccharomyces cerevisiae Rad14, which act in nucleotide excision repair of DNA damages induced by ultraviolet light and chemical agents. Cells with disrupted rhp14 were highly sensitive to ultraviolet light, and epistasis analysis with swi10 (nucleotide excision repair) and rad2 (Uve1-dependent ultraviolet light damage repair pathway) revealed that Rhp14 is an important component of nucleotide excision repair for ultraviolet light induced damages. Moreover, defective rhp14 caused instability of a GT repeat, similar to swi10, and synergistically with msh2 and exo1. Recombinant Rhp14 with an N-terminal hexahistidine-tag was purified from E. coli. Complementation studies with a rhp14 mutant demonstrated that the tagged Rhp14 is functional in repair of ultraviolet radiation induced damages and in mitotic mutation avoidance. In band shift assays, Rhp14 showed a preference to substrates with mismatched and unpaired nucleotides. Similarly, XPA bound more efficiently to C/C, A/C and T/C mismatches than to homoduplex DNA. Our data show that mismatches and loops in DNA are substrate of nucleotide excision repair. Rhp14 is likely part of the recognition complex, but alone not sufficient for the high discrimination of nucleotide excision repair for modified DNA.

Add to CiteULike CiteULike Add to Complore Complore Add to Connotea Connotea Add to Del.icio.us Del.icio.us Add to Digg Digg Add to Reddit Reddit Add to Technorati Technorati What's this?

This article has been cited by other articles:

K. M. Lee, S. Nizza, T. Hayes, K. L. Bass, A. Irmisch, J. M. Murray, and M. J. O'Connell
Brc1-Mediated Rescue of Smc5/6 Deficiency: Requirement for Multiple Nucleases and a Novel Rad18 Function
Genetics, April 1, 2007; 175(4): 1585 - 1595.
[Abstract] [Full Text] [PDF]

R. Muheim-Lenz, T. Buterin, G. Marra, and H. Naegeli
Short-patch correction of C/C mismatches in human cells
Nucleic Acids Res., December 21, 2004; 32(22): 6696 - 6705.
[Abstract] [Full Text] [PDF]

T. M. Marti, C. Kunz, and O. Fleck
Repair of Damaged and Mismatched DNA by the XPC Homologues Rhp41 and Rhp42 of Fission Yeast
Genetics, June 1, 2003; 164(2): 457 - 467.
[Abstract] [Full Text] [PDF]

All ASBMB Journals	Molecular and Cellular Proteomics
Journal of Lipid Research	ASBMB Today

				R. Muheim-Lenz, T. Buterin, G. Marra, and H. Naegeli Short-patch correction of C/C mismatches in human cells Nucleic Acids Res., December 21, 2004; 32(22): 6696 - 6705. [Abstract] [Full Text] [PDF]

				T. M. Marti, C. Kunz, and O. Fleck Repair of Damaged and Mismatched DNA by the XPC Homologues Rhp41 and Rhp42 of Fission Yeast Genetics, June 1, 2003; 164(2): 457 - 467. [Abstract] [Full Text] [PDF]