Papers In Press, published online ahead of print August 1, 2001
J. Biol. Chem, 10.1074/jbc.M104488200
Submitted on May 17, 2001
Revised on July 26, 2001
Accepted on July 31, 2001
Identification of the first trypanosome H/ACA RNA that guides pseudouridine formation on rRNA
Xue-hai Liang, Li Liu, and Shulamit Michaeli
Faculty of Life Sciences, Bar Ilan University, Ramat Gan 52900
Corresponding Author: michaes{at}mail.biu.ac.il
In trypanosomes snoRNA genes are clustered, and the clusters encode for either single or multiple RNAs. We previously reported on a genomic locus in Leptomonas collosoma that encodes for multiple C/D snoRNAs whose expression is regulated at the processing level (Xu, Y-X., Liu, L., and Michaeli, S. (2001) J. Biol. Chem. 276, 14289-14298). In this study we have characterized, in the same genomic locus, the first trypanosome H/ACA RNA, which we termed h1. Having a length of 69 nt, h1 has the potential to guide pseudouridylation on 28S rRNA. The h1 is processed from a long polycistronic transcript that carries both the C/D and h1 snoRNAs. The h1/rRNA duplex obeys the rules for guiding pseudouridylation. Mapping of the pseudouridine site indicated that the predicted U is indeed modified. However, in contrast to all H/ACA RNAs, h1 consists of a single hairpin structure and is the shortest H/ACA RNA described so far.
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