JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on January 4, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/1/630    most recent
M105144200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mizushina, Y.
Right arrow Articles by Sakaguchi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mizushina, Y.
Right arrow Articles by Sakaguchi, K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print October 24, 2001
J. Biol. Chem, 10.1074/jbc.M105144200
Submitted on June 5, 2001
Revised on October 4, 2001
Accepted on October 24, 2001

A plant phytotoxin, solanapyrone A, is an inhibitor of DNA polymerase beta and lambda

Yoshiyuki Mizushina, Shinji Kamisuki, Nobuyuki Kasai, Noriko Shimazaki, Masaharu Takemura, Hitomi Asahara, Stuart Linn, Shonen Yoshida, Akio Matsukage, Osamu Koiwai, Fumio Sugawara, Hiromi Yoshida, and Kengo Sakaguchi

Nutritional Science, Kobe-Gakuin University, Kobe, Hyogo 651-2180

Corresponding Author: mizushin{at}nutr.kobegakuin.ac.jp

Solanapyrone A, a phytotoxin and enzyme inhibitor isolated from a fungus (SUT 01B1-2), selectively inhibits the activities of mammalian DNA polymerase beta and lambda (pol beta and lambda) in vitro. The IC50 values of the compound were 30 micro M for pol beta and 37 micro M for pol lambda. Since pol beta and lambda are in a family and their three-dimensional structures are thought to be highly similar to each other, we used pol beta to analyze the biochemical relationship with solanapyrone A. On pol beta, solanapyrone A antagonistically competed with both the DNA template and the nucleotide substrate. BIAcore analysis demonstrated that solanapyrone A bound selectively to the N-terminal 8 kDa domain of pol beta. This domain is known to bind single-stranded DNA (ssDNA), provide 5'-phosphate recognition of gapped DNA, and cleave the sugar-phosphate bond 3' to an intact apurinic/apyrimidinic (AP) site (i.e. AP lyase activity), including 5'-deoxyribose phosphate lyase activity. Solanapyrone A inhibited the ssDNA binding activity, but did not influence the activities of the 5'-phosphate recognition in gapped DNA structures, and the AP lyase. Based on these results, the inhibitory mechanism of solanapyrone A is discussed.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
S. M. Hashimi, M. K. Wall, A. B. Smith, A. Maxwell, and R. G. Birch
The Phytotoxin Albicidin is a Novel Inhibitor of DNA Gyrase
Antimicrob. Agents Chemother., January 1, 2007; 51(1): 181 - 187.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
T. Takeuchi, T. Ishidoh, H. Iijima, I. Kuriyama, N. Shimazaki, O. Koiwai, K. Kuramochi, S. Kobayashi, F. Sugawara, K. Sakaguchi, et al.
Structural relationship of curcumin derivatives binding to the BRCT domain of human DNA polymerase {lambda}
Genes Cells, March 1, 2006; 11(3): 223 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
F. Boudsocq, P. Benaim, Y. Canitrot, M. Knibiehler, F. Ausseil, J. P. Capp, A. Bieth, C. Long, B. David, I. Shevelev, et al.
Modulation of Cellular Response to Cisplatin by a Novel Inhibitor of DNA Polymerase {beta}
Mol. Pharmacol., May 1, 2005; 67(5): 1485 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H.-Y. Hu, J. K. Horton, M. R. Gryk, R. Prasad, J. M. Naron, D.-A. Sun, S. M. Hecht, S. H. Wilson, and G. P. Mullen
Identification of Small Molecule Synthetic Inhibitors of DNA Polymerase {beta} by NMR Chemical Shift Mapping
J. Biol. Chem., September 17, 2004; 279(38): 39736 - 39744.
[Abstract] [Full Text] [PDF]

Home page
 J BiochemHome page
Y. Mizushina, C. Murakami, H. Takikawa, N. Kasai, X. Xu, K. Mori, M. Oshige, T. Yamaguchi, M. Saneyoshi, N. Shimazaki, et al.
Molecular Action Mode of Hippospongic Acid A, an Inhibitor of Gastrulation of Starfish Embryos
J. Biochem., April 1, 2003; 133(4): 541 - 552.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.