JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on September 7, 2001
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
276/37/34659    most recent
M106336200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Garcia-Diaz, M.
Right arrow Articles by Blanco, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garcia-Diaz, M.
Right arrow Articles by Blanco, L.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print July 16, 2001
J. Biol. Chem, 10.1074/jbc.M106336200
Submitted on July 6, 2001
Revised on July 16, 2001
Accepted on July 13, 2001

Identification of an intrinsic dRP lyase activity in human DNA polymerase Lambda: A possible role in base excision repair

Miguel Garcia-Diaz, Katarzyna Bebenek, Thomas A. Kunkel, and Luis Blanco

Centro de Biología Molecular Severo Ochoa, Madrid 28049

Corresponding Author: lblanco{at}cbm.uam.es

Base Excision Repair (BER) is a major repair pathway in eukaryotic cells, responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Pivotal to this process is the 5´-deoxyribose-5-phosphate lyase (dRP lyase) activity of DNA polymerase beta (Pol beta ). DNA polymerase lambda (Pol lambda ) is a recently identified eukaryotic DNA polymerase that is homologous to Pol beta . We show here that human Pol lambda exhibits dRP lyase -but not AP lyase- activity in vitro, and that this activity is consistent with a beta -elimination mechanism. Accordingly, a single amino acid substitution (K310A) eliminated more than 90% of the wild-type dRP lyase activity, thus suggesting that Lys310 of Pol lambda is the main nucleophile involved in the reaction. The dRP lyase activity of Pol lambda , in coordination with its polymerization activity, efficiently repaired uracil-containing DNA in an in vitro reconstituted BER reaction. These results suggest that Pol lambda may participate in "single-nucleotide" base excision repair in mammalian cells.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
B. Banos, J. M. Lazaro, L. Villar, M. Salas, and M. de Vega
Editing of misaligned 3'-termini by an intrinsic 3'-5' exonuclease activity residing in the PHP domain of a family X DNA polymerase
Nucleic Acids Res., October 1, 2008; 36(18): 5736 - 5749.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K.-H. Tang, M. Niebuhr, A. Aulabaugh, and M.-D. Tsai
Solution structures of 2 : 1 and 1 : 1 DNA polymerase-DNA complexes probed by ultracentrifugation and small-angle X-ray scattering
Nucleic Acids Res., February 11, 2008; 36(3): 849 - 860.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
C. N. Kundu, R. Balusu, A. S. Jaiswal, and S. Narayan
Adenomatous polyposis coli-mediated hypersensitivity of mouse embryonic fibroblast cell lines to methylmethane sulfonate treatment: implication of base excision repair pathways
Carcinogenesis, October 1, 2007; 28(10): 2089 - 2095.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
X. Wu and J. Stavnezer
DNA polymerase {beta} is able to repair breaks in switch regions and plays an inhibitory role during immunoglobulin class switch recombination
J. Exp. Med., July 9, 2007; 204(7): 1677 - 1689.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. W. Duym, K. A. Fiala, N. Bhatt, and Z. Suo
Kinetic Effect of a Downstream Strand and Its 5'-Terminal Moieties on Single Nucleotide Gap-filling Synthesis Catalyzed by Human DNA Polymerase {lambda}
J. Biol. Chem., November 24, 2006; 281(47): 35649 - 35655.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. C. Foley, K. Arora, and T. Schlick
Sequential Side-Chain Residue Motions Transform the Binary into the Ternary State of DNA Polymerase {lambda}
Biophys. J., November 1, 2006; 91(9): 3182 - 3195.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. A. Fiala, W. W. Duym, J. Zhang, and Z. Suo
Up-regulation of the Fidelity of Human DNA Polymerase {lambda} by Its Non-enzymatic Proline-rich Domain
J. Biol. Chem., July 14, 2006; 281(28): 19038 - 19044.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. J. Picher, M. Garcia-Diaz, K. Bebenek, L. C. Pedersen, T. A. Kunkel, and L. Blanco
Promiscuous mismatch extension by human DNA polymerase lambda
Nucleic Acids Res., June 28, 2006; 34(11): 3259 - 3266.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
K. K.L. Chan, Q.-M. Zhang, and G. L. Dianov
Base excision repair fidelity in normal and cancer cells
Mutagenesis, May 1, 2006; 21(3): 173 - 178.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
T. Takeuchi, T. Ishidoh, H. Iijima, I. Kuriyama, N. Shimazaki, O. Koiwai, K. Kuramochi, S. Kobayashi, F. Sugawara, K. Sakaguchi, et al.
Structural relationship of curcumin derivatives binding to the BRCT domain of human DNA polymerase {lambda}
Genes Cells, March 1, 2006; 11(3): 223 - 235.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. Frouin, M. Toueille, E. Ferrari, I. Shevelev, and U. Hubscher
Phosphorylation of human DNA polymerase {lambda} by the cyclin-dependent kinase Cdk2/cyclin A complex is modulated by its association with proliferating cell nuclear antigen
Nucleic Acids Res., September 20, 2005; 33(16): 5354 - 5361.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. K. Braithwaite, P. S. Kedar, L. Lan, Y. Y. Polosina, K. Asagoshi, V. P. Poltoratsky, J. K. Horton, H. Miller, G. W. Teebor, A. Yasui, et al.
DNA Polymerase {lambda} Protects Mouse Fibroblasts against Oxidative DNA Damage and Is Recruited to Sites of DNA Damage/Repair
J. Biol. Chem., September 9, 2005; 280(36): 31641 - 31647.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Gonzalez-Barrera, A. Sanchez, J. F. Ruiz, R. Juarez, A. J. Picher, G. Terrados, P. Andrade, and L. Blanco
Characterization of SpPol4, a unique X-family DNA polymerase in Schizosaccharomyces pombe
Nucleic Acids Res., August 24, 2005; 33(15): 4762 - 4774.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
R. N. Trivedi, K. H. Almeida, J. L. Fornsaglio, S. Schamus, and R. W. Sobol
The Role of Base Excision Repair in the Sensitivity and Resistance to Temozolomide-Mediated Cell Death
Cancer Res., July 15, 2005; 65(14): 6394 - 6400.
[Abstract] [Full Text] [PDF]

Home page
 GENES CELLSHome page
N. Shimazaki, T. Yazaki, T. Kubota, A. Sato, A. Nakamura, S. Kurei, S. Toji, K. Tamai, and O. Koiwai
DNA polymerase lambda directly binds to proliferating cell nuclear antigen through its confined C-terminal region
Genes Cells, July 1, 2005; 10(7): 705 - 715.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Bebenek, M. Garcia-Diaz, S. R. Patishall, and T. A. Kunkel
Biochemical Properties of Saccharomyces cerevisiae DNA Polymerase IV
J. Biol. Chem., May 20, 2005; 280(20): 20051 - 20058.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. K. Braithwaite, R. Prasad, D. D. Shock, E. W. Hou, W. A. Beard, and S. H. Wilson
DNA Polymerase {lambda} Mediates a Back-up Base Excision Repair Activity in Extracts of Mouse Embryonic Fibroblasts
J. Biol. Chem., May 6, 2005; 280(18): 18469 - 18475.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
F. Boudsocq, P. Benaim, Y. Canitrot, M. Knibiehler, F. Ausseil, J. P. Capp, A. Bieth, C. Long, B. David, I. Shevelev, et al.
Modulation of Cellular Response to Cisplatin by a Novel Inhibitor of DNA Polymerase {beta}
Mol. Pharmacol., May 1, 2005; 67(5): 1485 - 1492.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Maga, K. Ramadan, Giada. A. Locatelli, I. Shevelev, S. Spadari, and U. Hubscher
DNA Elongation by the Human DNA Polymerase {lambda} Polymerase and Terminal Transferase Activities Are Differentially Coordinated by Proliferating Cell Nuclear Antigen and Replication Protein A
J. Biol. Chem., January 21, 2005; 280(3): 1971 - 1981.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Hashimoto, Y. Tominaga, Y. Nakabeppu, and M. Moriya
Futile short-patch DNA base excision repair of adenine:8-oxoguanine mispair
Nucleic Acids Res., November 5, 2004; 32(19): 5928 - 5934.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
D. Wong and B. Demple
Modulation of the 5'-Deoxyribose-5-phosphate Lyase and DNA Synthesis Activities of Mammalian DNA Polymerase {beta} by Apurinic/Apyrimidinic Endonuclease 1
J. Biol. Chem., June 11, 2004; 279(24): 25268 - 25275.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
L. Haracska, L. Prakash, and S. Prakash
A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair
Genes & Dev., November 15, 2003; 17(22): 2777 - 2785.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Bebenek, M. Garcia-Diaz, L. Blanco, and T. A. Kunkel
The Frameshift Infidelity of Human DNA Polymerase {lambda}: IMPLICATIONS FOR FUNCTION
J. Biol. Chem., September 5, 2003; 278(36): 34685 - 34690.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Matsuda, B. J. Vande Berg, K. Bebenek, W. P. Osheroff, S. H. Wilson, and T. A. Kunkel
The Base Substitution Fidelity of DNA Polymerase {beta}-dependent Single Nucleotide Base Excision Repair
J. Biol. Chem., July 3, 2003; 278(28): 25947 - 25951.
[Abstract] [Full Text] [PDF]

Home page
 Sci Aging Knowl EnvironHome page
P. V. Shcherbakova, K. Bebenek, and T. A. Kunkel
Functions of Eukaryotic DNA Polymerases
Sci. Aging Knowl. Environ., February 26, 2003; 2003(8): re3 - 3.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Maga, G. Villani, K. Ramadan, I. Shevelev, N. T. Le Gac, L. Blanco, G. Blanca, S. Spadari, and U. Hubscher
Human DNA Polymerase lambda Functionally and Physically Interacts with Proliferating Cell Nuclear Antigen in Normal and Translesion DNA Synthesis
J. Biol. Chem., December 6, 2002; 277(50): 48434 - 48440.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Ramadan, I. V. Shevelev, G. Maga, and U. Hubscher
DNA Polymerase lambda from Calf Thymus Preferentially Replicates Damaged DNA
J. Biol. Chem., May 17, 2002; 277(21): 18454 - 18458.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J.-B. Duvauchelle, L. Blanco, R. P. P. Fuchs, and A. M. Cordonnier
Human DNA polymerase mu (Pol {micro}) exhibits an unusual replication slippage ability at AAF lesion
Nucleic Acids Res., May 1, 2002; 30(9): 2061 - 2067.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Kobayashi, M. Watanabe, Y. Okada, H. Sawa, H. Takai, M. Nakanishi, Y. Kawase, H. Suzuki, K. Nagashima, K. Ikeda, et al.
Hydrocephalus, Situs Inversus, Chronic Sinusitis, and Male Infertility in DNA Polymerase {lambda}-Deficient Mice: Possible Implication for the Pathogenesis of Immotile Cilia Syndrome
Mol. Cell. Biol., April 15, 2002; 22(8): 2769 - 2776.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Bertocci, A. De Smet, E. Flatter, A. Dahan, J.-C. Bories, C. Landreau, J.-C. Weill, and C.-A. Reynaud
Cutting Edge: DNA Polymerases {micro} and {lambda} Are Dispensable for Ig Gene Hypermutation
J. Immunol., April 15, 2002; 168(8): 3702 - 3706.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Garcia-Diaz, K. Bebenek, R. Sabariegos, O. Dominguez, J. Rodriguez, T. Kirchhoff, E. Garcia-Palomero, A. J. Picher, R. Juarez, J. F. Ruiz, et al.
DNA Polymerase lambda , a Novel DNA Repair Enzyme in Human Cells
J. Biol. Chem., April 5, 2002; 277(15): 13184 - 13191.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. S. DeMott, E. Beyret, D. Wong, B. C. Bales, J.-T. Hwang, M. M. Greenberg, and B. Demple
Covalent Trapping of Human DNA Polymerase beta by the Oxidative DNA Lesion 2-Deoxyribonolactone
J. Biol. Chem., March 1, 2002; 277(10): 7637 - 7640.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. M. J. Burgers, E. V. Koonin, E. Bruford, L. Blanco, K. C. Burtis, M. F. Christman, W. C. Copeland, E. C. Friedberg, F. Hanaoka, D. C. Hinkle, et al.
Eukaryotic DNA Polymerases: Proposal for a Revised Nomenclature
J. Biol. Chem., November 16, 2001; 276(47): 43487 - 43490.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.