JBC Origene Your Gene Company

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on March 8, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/11/8906    most recent
M106372200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marchand, C.
Right arrow Articles by Pommier, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marchand, C.
Right arrow Articles by Pommier, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print December 26, 2001
J. Biol. Chem, 10.1074/jbc.M106372200
Submitted on July 9, 2001
Revised on November 5, 2001
Accepted on December 25, 2001

Interaction of human nuclear topoisomerase I with guanosine-quartet-forming and guanosine-rich single-stranded DNA and RNA oligonucleotides

Christophe Marchand, Philippe Pourquier, Gary Laco, Naijie Jing, and Yves Pommier

Laboratory of Molecular Pharmacology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892

Corresponding Author: marchanc{at}mail.nih.gov

Human nuclear DNA topoisomerase I (top1) plays a crucial role in DNA replication, transcription and chromosome condensation. In this study, we show that intra- and inter-molecular guanosine-quartets (G-quartets) can inhibit top1-mediated DNA cleavage at a high affinity site. Top1-mediated DNA cleavage was also inhibited by a 16-mer single-stranded oligodeoxynucleotide (ODN) containing a G-rich sequence (G2T2G5TG2TG3) and by its RNA equivalent, which do not form G-quartet structures. Comparison of various single-stranded ODN for their ability to inhibit top1-mediated DNA cleavage indicated that G-rich sequences containing repeats of 2 or 3 consecutive guanines interspaced with thymines specifically inhibited top1. We also found that both single-stranded and G-quartet-forming ODNs bind to top1 without being cleaved by the enzyme. These results demonstrate that either DNA or RNA G-rich single-stranded and G-quartet-forming oligonucleotides can bind to top1 and prevent cleavage of duplex DNA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Molecular Cancer TherapeuticsHome page
N. Jing, Q. Zhu, P. Yuan, Y. Li, L. Mao, and D. J. Tweardy
Targeting signal transducer and activator of transcription 3 with G-quartet oligonucleotides: a potential novel therapy for head and neck cancer.
Mol. Cancer Ther., February 1, 2006; 5(2): 279 - 286.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Antony, P. B. Arimondo, J.-S. Sun, and Y. Pommier
Position- and orientation-specific enhancement of topoisomerase I cleavage complexes by triplex DNA structures
Nucleic Acids Res., October 4, 2004; 32(17): 5163 - 5173.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. Jing, Y. Li, W. Xiong, W. Sha, L. Jing, and D. J. Tweardy
G-Quartet Oligonucleotides: A New Class of Signal Transducer and Activator of Transcription 3 Inhibitors That Suppresses Growth of Prostate and Breast Tumors through Induction of Apoptosis
Cancer Res., September 15, 2004; 64(18): 6603 - 6609.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.