| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print November 1, 2001
Department of Microbiology, University of Virginia, Charlottesville, VA 22908
Corresponding Author: lfp2n{at}virginia.edu
The correct assembly of chromatin is necessary for the maintenance of genomic stability in eukaryotic cells. A critical step in the assembly of new chromatin is the cell-cycle regulated synthesis and nuclear import of core histones. Here we demonstrate that the nuclear import pathway of histone H3 and H4 is mediated by at least two karyopherins/importins, Kap123p and Kap121p. Cytosolic H4 is found associated with Kap123p and H3. Kap121p is also present in the H4-PrA associated fractions, albeit in lesser amounts than Kap123p, suggesting that this Kap serves as an additional import receptor. We further demonstrate that cytosolic Kap123p is associated with acetylated H3 and acetylated H4. H3 and H4 each contain a nuclear localization signal (NLS) in their amino-terminal domains. These amino-terminal domains were found to be essential for the nuclear accumulation of H3 and H4-GFP reporters. Each NLS mediated direct binding to Kap123p and Kap121p, and decreased nuclear accumulation of H3 and H4 NLS-GFP reporters was observed in specific kap mutant strains. H3 and H4 are the first histones to be assembled onto DNA, and these results show that their import is mediated by at least two import pathways.
J. Biol. Chem, 10.1074/jbc.M106845200
Submitted on July 19, 2001
Revised on November 1, 2001
Accepted on November 1, 2001
Pathways mediating the nuclear import of histone H3 and H4 in yeast
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. L. Mersfelder and M. R. Parthun Involvement of Hat1p (Kat1p) Catalytic Activity and Subcellular Localization in Telomeric Silencing J. Biol. Chem., October 24, 2008; 283(43): 29060 - 29068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Wagstaff, J. Y. Fan, M. A. De Jesus, D. J. Tremethick, and D. A. Jans Efficient gene delivery using reconstituted chromatin enhanced for nuclear targeting FASEB J, July 1, 2008; 22(7): 2232 - 2242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Choi, B. Kim, K. Heo, K. Kim, H. Kim, Y. Zhan, J. A. Ranish, and W. An Purification and Characterization of Cellular Proteins Associated with Histone H4 Tails J. Biol. Chem., July 20, 2007; 282(29): 21024 - 21031. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Blackwell Jr., S. T. Wilkinson, N. Mosammaparast, and L. F. Pemberton Mutational Analysis of H3 and H4 N Termini Reveals Distinct Roles in Nuclear Import J. Biol. Chem., July 13, 2007; 282(28): 20142 - 20150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fries, C. Betz, K. Sohn, S. Caesar, G. Schlenstedt, and S. M. Bailer A Novel Conserved Nuclear Localization Signal Is Recognized by a Group of Yeast Importins J. Biol. Chem., July 6, 2007; 282(27): 19292 - 19301. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Heo, B. Kim, K. Kim, J. Choi, H. Kim, Y. Zhan, J. A. Ranish, and W. An Isolation and Characterization of Proteins Associated with Histone H3 Tails in Vivo J. Biol. Chem., May 25, 2007; 282(21): 15476 - 15483. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zlatanova, C. Seebart, and M. Tomschik Nap1: taking a closer look at a juggler protein of extraordinary skills FASEB J, May 1, 2007; 21(7): 1294 - 1310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Goffin, S. Vodala, C. Fraser, J. Ryan, M. Timms, S. Meusburger, B. Catimel, E. C. Nice, P. A. Silver, C.-Y. Xiao, et al. The Unfolded Protein Response Transducer Ire1p Contains a Nuclear Localization Sequence Recognized by Multiple beta Importins Mol. Biol. Cell, December 1, 2006; 17(12): 5309 - 5323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. L. Timney, J. Tetenbaum-Novatt, D. S. Agate, R. Williams, W. Zhang, B. T. Chait, and M. P. Rout Simple kinetic relationships and nonspecific competition govern nuclear import rates in vivo J. Cell Biol., November 20, 2006; 175(4): 579 - 593. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Qin and M. R. Parthun Recruitment of the type B histone acetyltransferase hat1p to chromatin is linked to DNA double-strand breaks. Mol. Cell. Biol., May 1, 2006; 26(9): 3649 - 3658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Caesar, M. Greiner, and G. Schlenstedt Kap120 Functions as a Nuclear Import Receptor for Ribosome Assembly Factor Rpf1 in Yeast Mol. Cell. Biol., April 15, 2006; 26(8): 3170 - 3180. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kahle, M. Baake, D. Doenecke, and W. Albig Subunits of the Heterotrimeric Transcription Factor NF-Y Are Imported into the Nucleus by Distinct Pathways Involving Importin {beta} and Importin 13 Mol. Cell. Biol., July 1, 2005; 25(13): 5339 - 5354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Hodges, J. H. Leslie, N. Mosammaparast, Y. Guo, J. Shabanowitz, D. F. Hunt, and L. F. Pemberton Nuclear Import of TFIIB Is Mediated by Kap114p, a Karyopherin with Multiple Cargo-binding Domains Mol. Biol. Cell, July 1, 2005; 16(7): 3200 - 3210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Schaper, J. Franke, S. H. Meijsing, and A. E. Ehrenhofer-Murray Nuclear import of the histone acetyltransferase complex SAS-I in Saccharomyces cerevisiae J. Cell Sci., April 1, 2005; 118(7): 1473 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Mosammaparast, B. C. Del Rosario, and L. F. Pemberton Modulation of Histone Deposition by the Karyopherin Kap114 Mol. Cell. Biol., March 1, 2005; 25(5): 1764 - 1778. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Glowczewski, J. H. Waterborg, and J. G. Berman Yeast Chromatin Assembly Complex 1 Protein Excludes Nonacetylatable Forms of Histone H4 from Chromatin and the Nucleus Mol. Cell. Biol., December 1, 2004; 24(23): 10180 - 10192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Leslie, W. Zhang, B. L. Timney, B. T. Chait, M. P. Rout, R. W. Wozniak, and J. D. Aitchison Characterization of Karyopherin Cargoes Reveals Unique Mechanisms of Kap121p-Mediated Nuclear Import Mol. Cell. Biol., October 1, 2004; 24(19): 8487 - 8503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Poveda, M. Pamblanco, S. Tafrov, V. Tordera, R. Sternglanz, and R. Sendra Hif1 Is a Component of Yeast Histone Acetyltransferase B, a Complex Mainly Localized in the Nucleus J. Biol. Chem., April 16, 2004; 279(16): 16033 - 16043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Nikolaev, M.-F. Cochet, and B. Felenbok Nuclear Import of Zinc Binuclear Cluster Proteins Proceeds through Multiple, Overlapping Transport Pathways Eukaryot. Cell, April 1, 2003; 2(2): 209 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Marfatia, E. B. Crafton, D. M. Green, and A. H. Corbett Domain Analysis of the Saccharomyces cerevisiae Heterogeneous Nuclear Ribonucleoprotein, Nab2p. DISSECTING THE REQUIREMENTS FOR Nab2p-FACILITATED POLY(A) RNA EXPORT J. Biol. Chem., February 21, 2003; 278(9): 6731 - 6740. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Chua, C. Lingner, C. Frazer, and P. G. Young The sal3+ Gene Encodes an Importin-{beta} Implicated in the Nuclear Import of Cdc25 in Schizosaccharomyces pombe Genetics, October 1, 2002; 162(2): 689 - 703. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bauerle, D. Doenecke, and W. Albig The Requirement of H1 Histones for a Heterodimeric Nuclear Import Receptor J. Biol. Chem., August 30, 2002; 277(36): 32480 - 32489. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
