JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on February 8, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/7/5529    most recent
M107946200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yoshikawa, N.
Right arrow Articles by Tanaka, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yoshikawa, N.
Right arrow Articles by Tanaka, H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print December 10, 2001
J. Biol. Chem, 10.1074/jbc.M107946200
Submitted on August 17, 2001
Revised on November 27, 2001
Accepted on December 7, 2001

Distinct interaction of cortivazol with the ligand binding domain confers glucocorticoid receptor specificity

Noritada Yoshikawa, Yuichi Makino, Kensaku Okamoto, Chikao Morimoto, Isao Makino, and Hirotoshi Tanaka

Division of Clinical Immunology, Institute of Medical Science,, University of Tokyo, Minato-ku, Tokyo 108-8639

Corresponding Author: hirotnk{at}ims.u-tokyo.ac.jp

Ligand-receptor coupling is one of the important constituents of signal transduction and essential for physiological transmission of actions of endogenous substances including steroid hormones. However, molecular mechanisms of the redundancy between glucocorticoid and mineralocorticoid remains unknown because of complicated cross-talk among, for example, these adrenal steroids, their cognate receptors, and target genes. Receptor-specific ligand that can distinctly modulate target gene expression should be developed to overcome this issue. In this report, we showed that a pyrazolosteroid cortivazol (CVZ) does not induce either nuclear translocation or transactivation function of the mineralocorticoid receptor (MR), but does both of the glucocorticoid receptor (GR). Moreover, deletion analysis of the C-terminal end of the GR has revealed that CVZ interacts with the distinct portion of the ligand binding domain (LBD) and differentially modulates the ligand-dependent interaction between TIF2 and the LBD when compared with cortisol, dexamethasone, and aldosterone. It, thus, is indicated that CVZ may be not only a molecular probe for the analysis of the redundancy between the GR and MR in vivo but also a useful reagent to clarify structure-function relationship of the GR LBD.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
S. Sola, J. D. Amaral, P. M. Borralho, R. M. Ramalho, R. E. Castro, M. M. Aranha, C. J. Steer, and C. M. P. Rodrigues
Functional Modulation of Nuclear Steroid Receptors by Tauroursodeoxycholic Acid Reduces Amyloid {beta}-Peptide-Induced Apoptosis
Mol. Endocrinol., October 1, 2006; 20(10): 2292 - 2303.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
N. Shimizu, R. Ouchida, N. Yoshikawa, T. Hisada, H. Watanabe, K. Okamoto, M. Kusuhara, H. Handa, C. Morimoto, and H. Tanaka
HEXIM1 forms a transcriptionally abortive complex with glucocorticoid receptor without involving 7SK RNA and positive transcription elongation factor b
PNAS, June 14, 2005; 102(24): 8555 - 8560.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
N. Yoshikawa, K. Yamamoto, N. Shimizu, S. Yamada, C. Morimoto, and H. Tanaka
The Distinct Agonistic Properties of the Phenylpyrazolosteroid Cortivazol Reveal Interdomain Communication within the Glucocorticoid Receptor
Mol. Endocrinol., May 1, 2005; 19(5): 1110 - 1124.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Kodama, N. Shimizu, N. Yoshikawa, Y. Makino, R. Ouchida, K. Okamoto, T. Hisada, H. Nakamura, C. Morimoto, and H. Tanaka
Role of the Glucocorticoid Receptor for Regulation of Hypoxia-dependent Gene Expression
J. Biol. Chem., August 29, 2003; 278(35): 33384 - 33391.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.