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Papers In Press, published online ahead of print November 5, 2001
Department of Medical Biochemistry, University of Geneva Medical School, Geneva, Geneva-4 1211
Corresponding Author: martine.collart{at}medecine.unige.ch
The Ccr4-Not complex is a global regulator of transcription that affects genes positively and negatively, and is thought to regulate TFIID function. Two components of this complex, Caf1p and Ccr4p, are directly involved in mRNA deadenylation, and Caf1p is associated with Dhh1p, a putative RNA helicase thought to be a component of the decapping complex. In this work we tried to determine whether Dhh1p might interact with the Ccr4-Not complex. We found that, first, not mutations displayed severe synthetic phenotypes when combined with a dhh1 null mutation. Second, overexpression of Not1p was toxic in dhh1 null cells. Third, a not mutant phenotype was suppressed by deletion of DHH1 and mimicked by overexpression of DHH1. Fourth, dhh1 null mutants displayed resistance to heat shock, a phenotype observed for all mutants that affect the Ccr4-Not complex. Finally, like Caf1p and Ccr4p, Dhh1p co-immunoprecipitated with the non-essential N-terminal domain of Not1p and the levels of Caf1p and Dhh1p were dependent upon this Not1p domain. Taken together, our results suggest that the Ccr4-Not complex via the N-terminal region of Not1p, is necessary for the maintenance of stable cellular levels of Dhh1p and Caf1p, thus contributing to regulation of mRNA decay in addition to transcription.
J. Biol. Chem, 10.1074/jbc.M107979200
Submitted on August 20, 2001
Revised on November 5, 2001
Accepted on November 5, 2001
Interaction between Not1p, a component of the Ccr4-Not complex, a global regulator of transcription, and Dhh1p, a putative RNA helicase
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