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Papers In Press, published online ahead of print September 18, 2001
Molecular Genetics, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City 04510
Corresponding Author: amanjarr{at}ifisiol.unam.mx
In the yeast Saccharomyces cerevisiae, two NADP+-dependent glutamate dehydrogenases (NADP-GDH) encoded by GDH1 and GDH3, catalyze the synthesis of glutamate from ammonium and
J. Biol. Chem, 10.1074/jbc.M107986200
Submitted on August 20, 2001
Revised on September 17, 2001
Accepted on September 18, 2001
NADP-glutamate dehydrogenase isoenzymes of Saccharomyces cerevisiae. Purification, kinetic properties, and physiological roles
-ketoglutarate. The GDH2-encoded NAD+-dependent glutamate dehydrogenase degrades glutamate producing ammonium and -ketoglutarate. Until very recently, it was considered that only one biosynthetic NADP-GDH was present in S. cerevisiae. This fact hindered understanding the physiological role of each isoenzyme and the mechanisms involved in -ketoglutarate channeling for glutamate biosynthesis. In this study we purified and characterized the GDH1- and GDH3-encoded NADP-GDHs; they showed different allosteric properties and rates of -ketoglutarate utilization. Analysis of the relative levels of these proteins revealed that the expression of GDH1 and GDH3 is differentially regulated and depends on the nature of the carbon source. Moreover, the physiological study of mutants lacking or overexpressing GDH1 or GDH3 suggested that these genes play non-redundant physiological roles. Our results indicate that the coordinated regulation of GDH1, GDH3 and GDH2-encoded enzymes results in glutamate biosynthesis and balanced utilization of -ketoglutarate under fermentative and respiratory conditions. The possible relevance of the duplicated NADP-GDH pathway in the adaptation to facultative metabolism is discussed.
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