JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on March 1, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/10/8406    most recent
M108306200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miller, K. A.
Right arrow Articles by Albala, J. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, K. A.
Right arrow Articles by Albala, J. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print December 13, 2001
J. Biol. Chem, 10.1074/jbc.M108306200
Submitted on August 28, 2001
Revised on December 11, 2001
Accepted on December 12, 2001

RAD51C interacts with RAD51B and is central to a larger protein complex in vivo exclusive of RAD51

Kristi A. Miller, Daniel M. Yoshikawa, Ian R. McConnell, Robin Clark, David Schild, and Joanna S. Albala

Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, CA 94550

Corresponding Author: albala1{at}llnl.gov

RAD51B and RAD51C are two of five known paralogs of the human RAD51 protein which are thought to function in both homologous recombination and DNA double-strand break repair. This work describes the in vitro and in vivo identification of the RAD51B/RAD51C heterocomplex. The RAD51B/RAD51C heterocomplex was isolated and purified by immunoaffinity chromatography from insect cells co-expressing the recombinant proteins. Moreover, co-immunoprecipitation of the RAD51B and RAD51C proteins from HeLa, MCF10A, and MCF7 cells strongly suggests the existence of an endogenous RAD51B/RAD51C heterocomplex. We extended these observations to examine the interaction between the RAD51B/RAD51C complex and the other RAD51 paralogs. Immunoprecipitation using protein-specific antibodies showed that RAD51C is central to a single, large protein complex and/or several smaller complexes with RAD51B, RAD51D, XRCC2, and XRCC3. However, our experiments showed no evidence for the inclusion of RAD51 within these complexes. Further analysis is required to elucidate the function of the RAD51B/RAD51C heterocomplex and its association with the other RAD51 paralogs in the processes of homologous recombination and DNA double-strand break repair.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 GENES CELLSHome page
E. Mladenov, B. Anachkova, and I. Tsaneva
Sub-nuclear localization of Rad51 in response to DNA damage.
Genes Cells, May 1, 2006; 11(5): 513 - 524.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Wiese, J. M. Hinz, R. S. Tebbs, P. B. Nham, S. S. Urbin, D. W. Collins, L. H. Thompson, and D. Schild
Disparate requirements for the Walker A and B ATPase motifs of human RAD51D in homologous recombination.
Nucleic Acids Res., January 1, 2006; 34(9): 2833 - 2843.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
A. M. Gruver, K. A. Miller, C. Rajesh, P. G. Smiraldo, S. Kaliyaperumal, R. Balder, K. M. Stiles, J. S. Albala, and D. L. Pittman
The ATPase motif in RAD51D is required for resistance to DNA interstrand crosslinking agents and interaction with RAD51C
Mutagenesis, November 1, 2005; 20(6): 433 - 440.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
Y. Yonetani, H. Hochegger, E. Sonoda, S. Shinya, H. Yoshikawa, S. Takeda, and M. Yamazoe
Differential and collaborative actions of Rad51 paralog proteins in cellular response to DNA damage
Nucleic Acids Res., August 10, 2005; 33(14): 4544 - 4552.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. G. Smiraldo, A. M. Gruver, J. C. Osborn, and D. L. Pittman
Extensive Chromosomal Instability in Rad51d-Deficient Mouse Cells
Cancer Res., March 15, 2005; 65(6): 2089 - 2096.
[Abstract] [Full Text] [PDF]

Home page
 MutagenesisHome page
K. A. Miller, J. M. Hinz, N.A. Yamada, L. H. Thompson, and J. S. Albala
Nuclear localization of Rad51B is independent of Rad51C and BRCA2
Mutagenesis, January 1, 2005; 20(1): 57 - 63.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-C. Lio, D. Schild, M. A. Brenneman, J. L. Redpath, and D. J. Chen
Human Rad51C Deficiency Destabilizes XRCC3, Impairs Recombination, and Radiosensitizes S/G2-phase Cells
J. Biol. Chem., October 1, 2004; 279(40): 42313 - 42320.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
N. A. Yamada, J. M. Hinz, V. L. Kopf, K. D. Segalle, and L. H. Thompson
XRCC3 ATPase Activity Is Required for Normal XRCC3-Rad51C Complex Dynamics and Homologous Recombination
J. Biol. Chem., May 28, 2004; 279(22): 23250 - 23254.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Yokoyama, N. Sarai, W. Kagawa, R. Enomoto, T. Shibata, H. Kurumizaka, and S. Yokoyama
Preferential binding to branched DNA strands and strand-annealing activity of the human Rad51B, Rad51C, Rad51D and Xrcc2 protein complex
Nucleic Acids Res., May 11, 2004; 32(8): 2556 - 2565.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
A. Mohindra, E. Bolderson, J. Stone, M. Wells, T. Helleday, and M. Meuth
A tumour-derived mutant allele of XRCC2 preferentially suppresses homologous recombination at DNA replication forks
Hum. Mol. Genet., January 15, 2004; 13(2): 203 - 212.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
Y. Liu, J.-Y. Masson, R. Shah, P. O'Regan, and S. C. West
RAD51C Is Required for Holliday Junction Processing in Mammalian Cells
Science, January 9, 2004; 303(5655): 243 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. A. Miller, D. Sawicka, D. Barsky, and J. S. Albala
Domain mapping of the Rad51 paralog protein complexes
Nucleic Acids Res., January 2, 2004; 32(1): 169 - 178.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. A. French, C. E. Tambini, and J. Thacker
Identification of Functional Domains in the RAD51L2 (RAD51C) Protein and Its Requirement for Gene Conversion
J. Biol. Chem., November 14, 2003; 278(46): 45445 - 45450.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. Kurumizaka, R. Enomoto, M. Nakada, K. Eda, S. Yokoyama, and T. Shibata
Region and amino acid residues required for Rad51C binding in the human Xrcc3 protein
Nucleic Acids Res., July 15, 2003; 31(14): 4041 - 4050.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-C. Lio, A. V. Mazin, S. C. Kowalczykowski, and D. J. Chen
Complex Formation by the Human Rad51B and Rad51C DNA Repair Proteins and Their Activities in Vitro
J. Biol. Chem., January 17, 2003; 278(4): 2469 - 2478.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Yokoyama, H. Kurumizaka, S. Ikawa, S. Yokoyama, and T. Shibata
Holliday Junction Binding Activity of the Human Rad51B Protein
J. Biol. Chem., January 17, 2003; 278(4): 2767 - 2772.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. A. French, J.-Y. Masson, C. S. Griffin, P. O'Regan, S. C. West, and J. Thacker
Role of Mammalian RAD51L2 (RAD51C) in Recombination and Genetic Stability
J. Biol. Chem., May 24, 2002; 277(22): 19322 - 19330.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
H. Kurumizaka, S. Ikawa, M. Nakada, R. Enomoto, W. Kagawa, T. Kinebuchi, M. Yamazoe, S. Yokoyama, and T. Shibata
Homologous Pairing and Ring and Filament Structure Formation Activities of the Human Xrcc2{middle dot}Rad51D Complex
J. Biol. Chem., April 12, 2002; 277(16): 14315 - 14320.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.