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M108433200v1
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Papers In Press, published online ahead of print December 13, 2001
J. Biol. Chem, 10.1074/jbc.M108433200
Submitted on August 31, 2001
Revised on December 13, 2001
Accepted on December 13, 2001

Nuclear localization of duplin, a beta -catenin-binding protein, is essential for its inhibitory activity on the Wnt signaling pathway

Masashi Kobayashi, Shosei Kishida, Akimasa Fukui, Tatsuo Michiue, Yoichi Miyamoto, Tetsuji Okamoto, Yoshihiro Yoneda, Makoto Asashima, and Akira Kikuchi

Department of Biochemistry, Hiroshima University School of Medicine, Hiroshima, Hiroshima 734-8551

Corresponding Author: akikuchi{at}hiroshima-u.ac.jp

Duplin binds to beta -catenin and inhibits the Wnt signaling pathway, thereby leading to repression of the beta -catenin-mediated transactivation and Xenopus axis formation. To find an additional function of Duplin, yeast two-hybrid screening was carried out. Importin alpha was isolated as a binding protein of Duplin. Importin alpha bound directly to basic amino acid clusters of Duplin. Although Duplin was present in the nucleus, deletion of the basic amino acid clusters (Duplin{delta500-584}) retained Duplin in the cytoplasm. Duplin{delta500-584} bound to beta -catenin as efficiently as wild-type Duplin, but it neither repressed Wnt-dependent Tcf transcriptional activation in mammalian cells nor showed ventralization in Xenopus embryos. The Duplin mutant without a beta -catenin-binding region lost the ability to inhibit the Wnt-dependent Tcf activation, but retained its ventralizing activity. Furthermore, Duplin not only suppressed beta -catenin-dependent axis duplication and expression of siamois, a Wnt-regulated gene, but also inhibited siamois-dependent axis duplication. These results indicate that Duplin is translocated to the nucleus by interacting with importin alpha , and that nuclear localization is essential for the function of Duplin. Moreover, Duplin has an additional activity of inhibiting the Wnt signaling pathway by affecting the downstream of beta -catenin target genes.


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