JBC Avanti Polar Lipids

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on December 7, 2001
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
276/50/47650    most recent
M108719200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wakino, S.
Right arrow Articles by Law, R. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wakino, S.
Right arrow Articles by Law, R. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print October 11, 2001
J. Biol. Chem, 10.1074/jbc.M108719200
Submitted on September 10, 2001
Revised on October 11, 2001
Accepted on October 11, 2001

PPARgamma ligands inhibit mitogenic induction of p21Cip1 by modulating the PKC pathway in vascular smooth muscle cells

Shu Wakino, Ulrich Kintscher, Zhaowei Liu, Sarah Kim, Fen Yin, Motoi Ohba, Toshio Kuroki, Axel H. Schönthal, Willa A. Hsueh, and Ronald E. Law

Division of Endocrinology, UCLA, Los Angeles, CA 90095-7073

Corresponding Author: rlaw{at}mednet.ucla.edu

The cyclin-dependent kinase inhibitor p21Cip1 is upregulated in response to mitogenic stimulation in various cells. PPARgamma ligands, troglitazone (TRO 10 mu M) and rosiglitazone (RSG 10 mu M) attenuated the induction of p21Cip1 protein by PDGF and insulin without affecting cognate mRNA levels in rat aortic smooth muscle cells (RASMC). The PKCdelta inhibitor rottlerin also blocked the induction of p21Cip1 protein, whereas the conventional PKC isotype inhibitor Gö 6976 had no effect. Kinetic studies using the protein synthesis inhibitor cycloheximide showed that TRO, RSG, and rottlerin shortened the half-life of p21Cip1 protein. TRO, RSG, and rottlerin inhibited PDGF-induced expression of p21Cip1, whereas they did not affect insulin-induced expression of p21Cip1. Both ligands inhibited PKCdelta enzymatic activity in PDGF-stimulated RASMC, but not in insulin-stimulated cells. Adenoviral mediated overexpression of PKCdelta rescued the downregulation of p21Cip1 expression both by TRO and RSG in PDGF-treated RASMC. These data suggested that the PKCdelta pathway plays a critical role in PDGF-induced expression of p21Cip1 in RASMC and may be the potential target for PPARgamma ligand effects. Src-kinase dependent tyrosine-phosphorylation of PKCdelta was substantially decreased by TRO and RSG. Tyrosine phosphorylation and activation of c-Src in response to PDGF was unaffected by either PPARgamma ligand. Protein tyrosine phosphatase (PTPase) inhibitors sodium orthovanadate and dephostatin prevented PPARgamma ligand effects on PKCdelta tyrosine phosphrylation and enzymatic activity. Both PTPase inhibitors also reversed PPARgamma ligand effects on p21Cip1 expression in PDGF-treated RASMC. PPARgamma ligands enhanced PTPase activity in RASMC, which may be the mechanism for decreased PKCdelta tyrosine phosphorylation and activity. PPARgamma ligands regulate p21Cip1 at a post-translational level by blocking PKCdelta signaling and accelerating p21Cip1 turnover.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Circ. Res.Home page
S. Z. Duan, M. G. Usher, and R. M. Mortensen
Peroxisome Proliferator-Activated Receptor-{gamma}-Mediated Effects in the Vasculature
Circ. Res., February 15, 2008; 102(3): 283 - 294.
[Abstract] [Full Text] [PDF]

Home page
 HypertensionHome page
H. Nakashima, G. D. Frank, H. Shirai, A. Hinoki, S. Higuchi, H. Ohtsu, K. Eguchi, A. Sanjay, M. E. Reyland, P. J. Dempsey, et al.
Novel Role of Protein Kinase C-{delta} Tyr311 Phosphorylation in Vascular Smooth Muscle Cell Hypertrophy by Angiotensin II
Hypertension, February 1, 2008; 51(2): 232 - 238.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
K. Hasegawa, S. Wakino, S. Tatematsu, K. Yoshioka, K. Homma, N. Sugano, M. Kimoto, K. Hayashi, and H. Itoh
Role of Asymmetric Dimethylarginine in Vascular Injury in Transgenic Mice Overexpressing Dimethylarginie Dimethylaminohydrolase 2
Circ. Res., July 20, 2007; 101(2): e2 - e10.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
J. T. Crossno Jr., C. V. Garat, J. E. B. Reusch, K. G. Morris, E. C. Dempsey, I. F. McMurtry, K. R. Stenmark, and D. J. Klemm
Rosiglitazone attenuates hypoxia-induced pulmonary arterial remodeling
Am J Physiol Lung Cell Mol Physiol, April 1, 2007; 292(4): L885 - L897.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
G. Hansmann, R. A. Wagner, S. Schellong, V. A. de Jesus Perez, T. Urashima, L. Wang, A. Y. Sheikh, R. S. Suen, D. J. Stewart, and M. Rabinovitch
Pulmonary Arterial Hypertension Is Linked to Insulin Resistance and Reversed by Peroxisome Proliferator-Activated Receptor-{gamma} Activation
Circulation, March 13, 2007; 115(10): 1275 - 1284.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
L. Wu, R. Vikramadithyan, S. Yu, C. Pau, Y. Hu, I. J. Goldberg, and H. M. Dansky
Addition of dietary fat to cholesterol in the diets of LDL receptor knockout mice: effects on plasma insulin, lipoproteins, and atherosclerosis
J. Lipid Res., October 1, 2006; 47(10): 2215 - 2222.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. Vazquez, T. Greenwell-Wild, N. J. Marinos, W. D. Swaim, S. Nares, D. E. Ott, U. Schubert, P. Henklein, J. M. Orenstein, M. B. Sporn, et al.
Human Immunodeficiency Virus Type 1-Induced Macrophage Gene Expression Includes the p21 Gene, a Target for Viral Regulation
J. Virol., April 1, 2005; 79(7): 4479 - 4491.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
L. Ragolia, T. Palaia, T. B. Koutrouby, and J. K. Maesaka
Inhibition of cell cycle progression and migration of vascular smooth muscle cells by prostaglandin D2 synthase: resistance in diabetic Goto-Kakizaki rats
Am J Physiol Cell Physiol, November 1, 2004; 287(5): C1273 - C1281.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Amini, M. Saunders, K. Kelley, K. Khalili, and B. E. Sawaya
Interplay between HIV-1 Vpr and Sp1 Modulates p21WAF1 Gene Expression in Human Astrocytes
J. Biol. Chem., October 29, 2004; 279(44): 46046 - 46056.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
S. Wakino, K. Hayashi, T. Kanda, S. Tatematsu, K. Homma, K. Yoshioka, I. Takamatsu, and T. Saruta
Peroxisome Proliferator-Activated Receptor {gamma} Ligands Inhibit Rho/Rho Kinase Pathway by Inducing Protein Tyrosine Phosphatase SHP-2
Circ. Res., September 3, 2004; 95(5): e45 - e55.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. V. Stahelin, M. A. Digman, M. Medkova, B. Ananthanarayanan, J. D. Rafter, H. R. Melowic, and W. Cho
Mechanism of Diacylglycerol-induced Membrane Targeting and Activation of Protein Kinase C{delta}
J. Biol. Chem., July 9, 2004; 279(28): 29501 - 29512.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
E. Verrier, L. Wang, C. Wadham, N. Albanese, C. Hahn, J. R. Gamble, V. K. K. Chatterjee, M. A. Vadas, and P. Xia
PPAR{gamma} Agonists Ameliorate Endothelial Cell Activation via Inhibition of Diacylglycerol-Protein Kinase C Signaling Pathway: Role of Diacylglycerol Kinase
Circ. Res., June 11, 2004; 94(11): 1515 - 1522.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. Nobe, H. Suzuki, Y. Sakai, H. Nobe, R. J. Paul, and K. Momose
Glucose-Dependent Enhancement of Spontaneous Phasic Contraction Is Suppressed in Diabetic Mouse Portal Vein: Association with Diacylglycerol-Protein Kinase C Pathway
J. Pharmacol. Exp. Ther., June 1, 2004; 309(3): 1263 - 1272.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
D. N. JACKSON and D. A. FOSTER
The enigmatic protein kinase C{delta}: complex roles in cell proliferation and survival
FASEB J, April 1, 2004; 18(6): 627 - 636.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. Baek, J.-S. Kim, J. B. Nixon, R. P. DiAugustine, and T. E. Eling
Expression of NAG-1, a Transforming Growth Factor-{beta} Superfamily Member, by Troglitazone Requires the Early Growth Response Gene EGR-1
J. Biol. Chem., February 20, 2004; 279(8): 6883 - 6892.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. F. Badawi, M. B. Eldeen, Y. Liu, E. A. Ross, and M. Z. Badr
Inhibition of Rat Mammary Gland Carcinogenesis by Simultaneous Targeting of Cyclooxygenase-2 and Peroxisome Proliferator-activated Receptor {gamma}
Cancer Res., February 1, 2004; 64(3): 1181 - 1189.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Y. Lin, X. Zhu, F. L. Mclntee, H. Xiao, J. Zhang, M. Fu, and Y. E. Chen
Interferon Regulatory Factor-1 Mediates PPAR{gamma}-Induced Apoptosis in Vascular Smooth Muscle Cells
Arterioscler. Thromb. Vasc. Biol., February 1, 2004; 24(2): 257 - 263.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
D. Bruemmer, F. Yin, J. Liu, J. P. Berger, T. Sakai, F. Blaschke, E. Fleck, A. J. Van Herle, B. M. Forman, and R. E. Law
Regulation of the Growth Arrest and DNA Damage-Inducible Gene 45 (GADD45) by Peroxisome Proliferator-Activated Receptor {gamma} in Vascular Smooth Muscle Cells
Circ. Res., August 22, 2003; 93 (4): e38 - e47.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. J. Baek, L. C. Wilson, L. C. Hsi, and T. E. Eling
Troglitazone, a Peroxisome Proliferator-activated Receptor gamma (PPARgamma ) Ligand, Selectively Induces the Early Growth Response-1 Gene Independently of PPARgamma . A NOVEL MECHANISM FOR ITS ANTI-TUMORIGENIC ACTIVITY
J. Biol. Chem., February 14, 2003; 278(8): 5845 - 5853.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
F. Nakao, S. Kobayashi, K. Mogami, Y. Mizukami, S. Shirao, S. Miwa, N. Todoroki-Ikeda, M. Ito, and M. Matsuzaki
Involvement of Src Family Protein Tyrosine Kinases in Ca2+ Sensitization of Coronary Artery Contraction Mediated by a Sphingosylphosphorylcholine-Rho-Kinase Pathway
Circ. Res., November 15, 2002; 91(10): 953 - 960.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
O. Barbier, I. P. Torra, Y. Duguay, C. Blanquart, J.-C. Fruchart, C. Glineur, and B. Staels
Pleiotropic Actions of Peroxisome Proliferator-Activated Receptors in Lipid Metabolism and Atherosclerosis
Arterioscler. Thromb. Vasc. Biol., May 1, 2002; 22(5): 717 - 726.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Zhang, M. Fu, X. Zhu, Y. Xiao, Y. Mou, H. Zheng, M. A. Akinbami, Q. Wang, and Y. E. Chen
Peroxisome Proliferator-activated Receptor delta Is Up-regulated during Vascular Lesion Formation and Promotes Post-confluent Cell Proliferation in Vascular Smooth Muscle Cells
J. Biol. Chem., March 22, 2002; 277(13): 11505 - 11512.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2001 by the American Society for Biochemistry and Molecular Biology.