| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print December 13, 2001
Institut für Klinische u. Molekulare Virologie, Erlangen, Bayern 91054
Corresponding Author: eebogner{at}viro.med.uni-erlangen.de
Herpesviral DNA packaging is a complex process resulting in unit-length genomes packed into preformed procapsids. This process is believed to be mediated by two packaging proteins, the terminase subunits. In the case of dsDNA bacteriophages, the translocation of DNA was shown to be an energy-dependent process associated with an ATPase activity of the large terminase subunit. In the case of human cytomegalovirus (HCMV) it was not known which protein has the ability to hydrolyze ATP. In this study we expressed HCMV terminase subunits, the carboxy-terminal half of pUL56 and pUL89, as GST-fusion proteins and purified these by affinity chromatography. ATPase assays demonstrated that the enzymatic activity is exclusively associated with pUL56. The characterization of the ATP hydrolysis showed that the enzymatic reaction is a fast process, whereas the spontaneous ATP decay followed slow kinetics. Interestingly, although pUL89 did not show any ATPase activity, it was capable of enhancing the UL56-associated ATP hydrolysis. Furthermore, a specific association of in vitro translated pUL89 with the carboxy-terminal half of GST-UL56 was detected. This interaction was confirmed by co-immunoprecipitations of infected cells. Our results clearly demonstrated that (i) both terminase subunits interact with each other and (ii) that the subunit pUL56 has an ATPase activity.
J. Biol. Chem, 10.1074/jbc.M108984200
Submitted on September 17, 2001
Revised on November 13, 2001
Accepted on December 12, 2001
ATPase activity of the terminase subunit pUL56 of human cytomegalovirus
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. B. Wang and M. A. McVoy Mutagenesis of the murine cytomegalovirus M56 terminase gene J. Gen. Virol., November 1, 2008; 89(11): 2864 - 2868. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Xiao, H. Zhang, and P. Guo Novel mechanism of hexamer ring assembly in protein/RNA interactions revealed by single molecule imaging Nucleic Acids Res., November 1, 2008; 36(20): 6620 - 6632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. M. Borst, K. Wagner, A. Binz, B. Sodeik, and M. Messerle The Essential Human Cytomegalovirus Gene UL52 Is Required for Cleavage-Packaging of the Viral Genome J. Virol., March 1, 2008; 82(5): 2065 - 2078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. A. McVoy and D. E. Nixon Impact of 2-Bromo-5,6-Dichloro-1-{beta}-D-Ribofuranosyl Benzimidazole Riboside and Inhibitors of DNA, RNA, and Protein Synthesis on Human Cytomegalovirus Genome Maturation J. Virol., September 1, 2005; 79(17): 11115 - 11127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Xiao, W.-D. Moll, S. Guo, and P. Guo Binding of pRNA to the N-terminal 14 amino acids of connector protein of bacteriophage phi29 Nucleic Acids Res., May 10, 2005; 33(8): 2640 - 2649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. E. Adamo, J. Schroer, and T. Shenk Human Cytomegalovirus TRS1 Protein Is Required for Efficient Assembly of DNA-Containing Capsids J. Virol., October 1, 2004; 78(19): 10221 - 10229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Underwood, R. G. Ferris, D. W. Selleseth, M. G. Davis, J. C. Drach, L. B. Townsend, K. K. Biron, and F. L. Boyd Mechanism of Action of the Ribopyranoside Benzimidazole GW275175X against Human Cytomegalovirus Antimicrob. Agents Chemother., May 1, 2004; 48(5): 1647 - 1651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Nixon and M. A. McVoy Dramatic Effects of 2-Bromo-5,6-Dichloro-1-{beta}-D-Ribofuranosyl Benzimidazole Riboside on the Genome Structure, Packaging, and Egress of Guinea Pig Cytomegalovirus J. Virol., February 15, 2004; 78(4): 1623 - 1635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Williams, C. B. Hartline, N. L. Kushner, E. A. Harden, D. J. Bidanset, J. C. Drach, L. B. Townsend, M. R. Underwood, K. K. Biron, and E. R. Kern In Vitro Activities of Benzimidazole D- and L-Ribonucleosides against Herpesviruses Antimicrob. Agents Chemother., July 1, 2003; 47(7): 2186 - 2192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. White, N. D. Stow, A. H. Patel, M. Hughes, and V. G. Preston Herpes Simplex Virus Type 1 Portal Protein UL6 Interacts with the Putative Terminase Subunits UL15 and UL28 J. Virol., June 1, 2003; 77(11): 6351 - 6358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Scholz, S. Rechter, J. C. Drach, L. B. Townsend, and E. Bogner Identification of the ATP-binding site in the terminase subunit pUL56 of human cytomegalovirus Nucleic Acids Res., March 1, 2003; 31(5): 1426 - 1433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Scheffczik, C. G. W. Savva, A. Holzenburg, L. Kolesnikova, and E. Bogner The terminase subunits pUL56 and pUL89 of human cytomegalovirus are DNA-metabolizing proteins with toroidal structure Nucleic Acids Res., April 1, 2002; 30(7): 1695 - 1703. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
