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Papers In Press, published online ahead of print September 26, 2001
J. Biol. Chem, 10.1074/jbc.M109247200
Submitted on September 25, 2001
Revised on September 26, 2001
Accepted on September 25, 2001

RecA protein filaments disassemble in the 5' to 3' direction on single-stranded DNA

Julie M. Bork, Michael M. Cox, and Ross B. Inman

Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1544

Corresponding Author: cox{at}biochem.wisc.edu

RecA protein forms filaments on both single- and double-stranded DNA. Several studies confirm that filament extension occurs in the 5' to 3' direction on single-stranded DNA. These filaments also disassemble in an end-dependent fashion, and several indirect observations suggest that the disassembly occurs on the end opposite to that at which assembly occurs. By labeling the 5' end of single-stranded DNA with a segment of duplex DNA, we demonstrate unambiguously that RecA filaments disassemble uniquely in the 5' to 3' direction.


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