JBC Focus on PI3-Kinase with Echelon

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on March 8, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/11/9484    most recent
M109371200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stein, A. J.
Right arrow Articles by Geiger, J. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stein, A. J.
Right arrow Articles by Geiger, J. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print January 4, 2002
J. Biol. Chem, 10.1074/jbc.M109371200
Submitted on September 27, 2001
Revised on January 2, 2002
Accepted on January 4, 2002

The crystal structure and mechanism of 1L-myo-Inositol-1-phosphate synthase

Adam J. Stein and James H. Geiger

Department of Chemistry, Michigan State University, East Lansing, MI 48824

Corresponding Author: geiger{at}cem.msu.edu

1L-myo-inositol-1-phosphate synthase catalyzes the conversion of D-glucose 6-phosphate to 1-L-myo-inositol-1-phosphate, the first and rate limiting step in the biosynthesis of all inositol-containing compounds. It involves an oxidation, intramolecular aldol cyclization and reduction. We have determined the crystal structure of MIP synthase bound to NAD and bound to an inhibitor, 2-deoxy-glucitol-6-phosphate. While 58 amino acids are disordered in the unbound form of the enzyme in the vicinity of the active site, the inhibitor nucleates the folding of this domain in a striking example of induced fit, serving to completely encapsulate it within the enzyme. Three helices and a long beta strand are formed in this process. We postulate a mechanism for the conversion based on the structure of the inhibitor-bound complex.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
K. Ghosh Dastidar, S. Maitra, L. Goswami, D. Roy, K. P. Das, and A. L. Majumder
An Insight into the Molecular Basis of Salt Tolerance of L-myo-Inositol 1-P Synthase (PcINO1) from Porteresia coarctata (Roxb.) Tateoka, a Halophytic Wild Rice
Plant Physiology, April 1, 2006; 140(4): 1279 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
K. Neelon, Y. Wang, B. Stec, and M. F. Roberts
Probing the Mechanism of the Archaeoglobus fulgidus Inositol-1-phosphate Synthase
J. Biol. Chem., March 25, 2005; 280(12): 11475 - 11482.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. L. Miller, C. Q. Wenzel, C. Daniels, S. Larocque, J.-R. Brisson, and J. S. Lam
Biochemical Characterization of WbpA, a UDP-N-acetyl-D-glucosamine 6-Dehydrogenase Involved in O-antigen Biosynthesis in Pseudomonas aeruginosa PAO1
J. Biol. Chem., September 3, 2004; 279(36): 37551 - 37558.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Majee, S. Maitra, K. G. Dastidar, S. Pattnaik, A. Chatterjee, N. C. Hait, K. P. Das, and A. L. Majumder
A Novel Salt-tolerant L-myo-Inositol-1-phosphate Synthase from Porteresia coarctata (Roxb.) Tateoka, a Halophytic Wild Rice: MOLECULAR CLONING, BACTERIAL OVEREXPRESSION, CHARACTERIZATION, AND FUNCTIONAL INTROGRESSION INTO TOBACCO-CONFERRING SALT TOLERANCE PHENOTYPE
J. Biol. Chem., July 2, 2004; 279(27): 28539 - 28552.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
X. Jin, K. M. Foley, and J. H. Geiger
The Structure of the 1L-myo-inositol-1-phosphate Synthase-NAD+-2-deoxy-D-glucitol 6-(E)-Vinylhomophosphonate Complex Demands a Revision of the Enzyme Mechanism
J. Biol. Chem., April 2, 2004; 279(14): 13889 - 13895.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.