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Papers In Press, published online ahead of print December 3, 2001
Cellular & System Physiology, Kyushu University Graduate School of Medical Sciences, Fukuoka 812-8582
Corresponding Author: nabekura{at}mailserver.med.kyushu-u.ac.jp
Motoneurons receive a robust recurrent synaptic inhibition by GABA and glycine, which activate Cl- channels. Thus, Cl- homeostasis determines the efficacy of synaptic inhibition in the motoneurons. In situ hybridization reveals that the neuronal isoform potassium-chloride cotransporter (KCC2), major mechanism in maintaining a low Cl- concentration in neurons, is abundantly expressed in the facial, hypoglossal (XII) and spinal motoneurons innervating striated muscle. Whereas the dorsal vagal motoneurons (DMV) controlling smooth muscle exhibited a little expression of KCC2. It raises a general interest of the correlation between KCC2 expression and inhibitory postsynaptic potentials (IPSPs) performance in the native circuits. Intracellular and whole-cell patch recordings revealed that an activity-dependent depression of IPSPs and positive shifted of IPSP reversal potentials were more prominent in the DMV than XII. Cl- influx through Cl- channel was extruded more potently in the XII than DMV, suggesting that differences in Cl- extrusion account for these dynamic differences of IPSP. Cl- extrusion was inhibited by either furosemide or increasing extracellular potassium concentrations. Thus, the rigid maintenance of IPSP and rapid Cl- extrusion in the XII reflects an intense expression of KCC2. KCC2 expression may strongly influence the IPSP depression and functional properties of the motoneurons innervating striated muscles.
J. Biol. Chem, 10.1074/jbc.M109439200
Submitted on October 1, 2001
Revised on November 28, 2001
Accepted on December 3, 2001
Diversity of neuron-specific K+-Cl- cotransporter expression and inhibitory postsynaptic potential depression in rat motoneurons
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