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Papers In Press, published online ahead of print October 30, 2001
Radiology and Cancer Biology, Nagasaki University School of Dentistry, Nagasaki 852-8588
Corresponding Author: taku{at}net.nagasaki-u.ac.jp
Flap endonuclease 1 (FEN-1) is a 5’-3’ flap exo-/endonuclease that plays an important role in Okazaki fragment maturation, non-homologous end joining of double-stranded DNA breaks, and long-patch base excision repair. Here, we demonstrate that the wild-type FEN-1 binds tightly to chromatin in conjunction with PCNA recruitment after MMS treatment, and the nuclease-defective FEN-1 increased the sensitivity of the cells to methylmethane sulfonate (MMS) and to UV light, but not to ionizing radiation. In contrast, the cells expressing the nuclease-defective and PCNA-binding defective double mutant FEN-1 exhibited sensitivities similar to those in the cells expressing the wild-type FEN-1. MMS treatment caused a prolonged delay of S-phase progression and impairment in colony-forming activity of cells expressing nuclease-defective FEN-1. Comet assay demonstrated that DNA repair after MMS or UV treatment was impaired in the cells expressing nuclease-deficient, but not in the cells with double-mutated FEN-1. Taken together, these findings suggest that FEN-1 plays an essential role in the DNA repair processes in mammalian cells, and that this activity of FEN-1 is PCNA-dependent.
J. Biol. Chem, 10.1074/jbc.M109461200
Submitted on October 1, 2001
Revised on October 29, 2001
Accepted on October 30, 2001
Defective FEN-1 activity in mammalian cells is associated with impaired DNA repair and prolonged S-phase delay
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