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M110014200v1
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Papers In Press, published online ahead of print December 14, 2001
J. Biol. Chem, 10.1074/jbc.M110014200
Submitted on October 17, 2001
Revised on December 10, 2001
Accepted on December 14, 2001

Probing the conformational change of E. coli undecaprenyl pyrophosphate synthase during catalysis using an inhibitor and tryptophan mutants

Yi-Hung Chen, Annie P.-C. Chen, Chao-Tsen Chen, Andrew H.-J. Wang, and Po-Huang Liang

Institute of Biological Chemistry, Academia Sinica, Taipei 115

Corresponding Author: phliang{at}gate.sinica.edu.tw

Undecaprenyl pyrophosphate synthase (UPPs) catalyzes the consecutive condensation reactions of eight isopentenyl pyrophosphate (IPP) with farnesyl pyrophosphate (FPP) to generate C55 undecaprenyl pyrophosphate (UPP). In the present study, site-directed mutagenesis, fluorescence quenching and stopped-flow methods were utilized to examine the substrate binding and the protein conformational change. Farnesyl thiolopyrophosphate (FsPP), a FPP analogue, was synthesized to probe the enzyme inhibition and events associated with the protein fluorescence change. This compound with a much less labile thiolopyrophosphate shows Ki value of 0.2 micro-M in the inhibition of E. coli UPPs, and serves as a poor substrate with the kcat value (3.1 x 10-7 s-1) 107 times smaller than using FPP as the substrate. Reduction of protein intrinsic fluorescence was observed upon addition of FPP (or FsPP) to the UPPs solution. Moreover, fluorescence studies carried out using W91F and other mutant UPPs with Trp replaced by Phe, indicate that FPP binding mainly quenches the fluorescence of W91, a residue in the 3 helix that moves toward the active site during substrate binding. Using stopped-flow apparatus, a three-phase protein fluorescence change with time was observed by mixing the E•FPP complex with IPP in the presence of Mg2+. However, during the binding of E•FsPP with IPP, only the fastest phase was observed. These results suggest that the first phase is due to the IPP binding to E•FPP complex and the other two slow phases are originated from the protein conformational change. The two slow phases coincide with the time course of FPP chain elongation from C15 to C55 and product release, respectively.


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This article has been cited by other articles:


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