| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print May 2, 2002
Department of Pathology and Program in Molecular Biology, University of Colorado Health Sciences Center, Denver, CO 80262
Corresponding Author: dean.edwards{at}uchsc.edu
Previously, we and others reported that the high mobility group proteins, HMGB-1/-2, enhance DNA binding in vitro and transactivation in situ by the steroid hormone subgroup of nuclear receptors, but did not influence these functions of class II receptors. We show here that the DNA binding domain (DBD) is sufficient to account for the selective influence of HMGB-1/-2 on the steroid class of receptors. Furthermore, the use of chimeric DBDs reveals that this selectivity is dependent on the C-terminal extension (CTE), amino acid sequences adjacent to the zinc-finger core DBD. HMGB-1/-2 interacts directly with the DBDs of steroid, but not class II receptors, and this interaction requires the CTE. This in vitro interaction correlates with a requirement of the CTE for maximal HMGB-1/-2 enhancement of DNA binding in vitro and transcriptional activation in cells. Finally, class II receptor DBDs have a much higher intrinsic affinity for DNA than steroid receptor DBDs and this affinity difference is also dependent on the CTE. These results reveal the importance of the steroid receptor CTE for DNA binding affinity and functional response to HMGB-1/-2.
J. Biol. Chem, 10.1074/jbc.M110400200
Submitted on October 29, 2001
Revised on May 2, 2002
Accepted on May 2, 2002
The carboxyl-terminal extension (CTE) of the nuclear hormone receptor DNA binding domain determines interactions and functional response to the HMGB-1/-2 co-regulatory proteins
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. C. Roemer, J. Adelman, M. E. A. Churchill, and D. P. Edwards Mechanism of high-mobility group protein B enhancement of progesterone receptor sequence-specific DNA binding Nucleic Acids Res., June 1, 2008; 36(11): 3655 - 3666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Schultz-Norton, V. A. Gabisi, Y. S. Ziegler, I. X. McLeod, J. R. Yates, and A. M. Nardulli Interaction of estrogen receptor {alpha} with proliferating cell nuclear antigen Nucleic Acids Res., August 1, 2007; 35(15): 5028 - 5038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Schultz-Norton, K. A. Walt, Y. S. Ziegler, I. X. McLeod, J. R. Yates, L. T. Raetzman, and A. M. Nardulli The Deoxyribonucleic Acid Repair Protein Flap Endonuclease-1 Modulates Estrogen-Responsive Gene Expression Mol. Endocrinol., July 1, 2007; 21(7): 1569 - 1580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Buser, E. K. Gass-Handel, S. L. Wyszomierski, W. Doppler, S. A. Leonhardt, J. Schaack, J. M. Rosen, H. Watkin, S. M. Anderson, and D. P. Edwards Progesterone Receptor Repression of Prolactin/Signal Transducer and Activator of Transcription 5-Mediated Transcription of the {beta}-Casein Gene in Mammary Epithelial Cells Mol. Endocrinol., January 1, 2007; 21(1): 106 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Roemer, D. C. Donham, L. Sherman, V. H. Pon, D. P. Edwards, and M. E. A. Churchill Structure of the Progesterone Receptor-Deoxyribonucleic Acid Complex: Novel Interactions Required for Binding to Half-Site Response Elements Mol. Endocrinol., December 1, 2006; 20(12): 3042 - 3052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Y. Kim, E. M. Woo, Y. T. E. Chong, D. R. Homenko, and W. L. Kraus Acetylation of Estrogen Receptor {alpha} by p300 at Lysines 266 and 268 Enhances the Deoxyribonucleic Acid Binding and Transactivation Activities of the Receptor Mol. Endocrinol., July 1, 2006; 20(7): 1479 - 1493. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. E. Wardell, S. C. Kwok, L. Sherman, R. S. Hodges, and D. P. Edwards Regulation of the Amino-Terminal Transcription Activation Domain of Progesterone Receptor by a Cofactor-Induced Protein Folding Mechanism Mol. Cell. Biol., October 15, 2005; 25(20): 8792 - 8808. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shostak and K. R. Yamamoto Overlapping but Separable Determinants of DNA Binding and Nuclear Localization Map to the C-terminal End of the Caenorhabditis elegans DAF-12 DNA Binding Domain J. Biol. Chem., February 25, 2005; 280(8): 6554 - 6560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Das, R. C. Peterson, and W. M. Scovell High Mobility Group B Proteins Facilitate Strong Estrogen Receptor Binding to Classical and Half-Site Estrogen Response Elements and Relax Binding Selectivity Mol. Endocrinol., November 1, 2004; 18(11): 2616 - 2632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. S. Melvin, C. Harrell, J. S. Adelman, W. L. Kraus, M. Churchill, and D. P. Edwards The Role of the C-terminal Extension (CTE) of the Estrogen Receptor {alpha} and {beta} DNA Binding Domain in DNA Binding and Interaction with HMGB J. Biol. Chem., April 9, 2004; 279(15): 14763 - 14771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Li and B. W. O'Malley Unfolding the Action of Progesterone Receptors J. Biol. Chem., October 10, 2003; 278(41): 39261 - 39264. [Full Text] [PDF]
|
|
|
|
|
|
G.-Z. Liu, H. Wang, and Z. Wang Identification of a Highly Conserved Domain in the Androgen Receptor That Suppresses the DNA-binding Domain-DNA Interactions J. Biol. Chem., April 18, 2003; 278(17): 14956 - 14960. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
