| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print March 8, 2002
Harvard Medical School, Center for Blood Research, Boston, MA 02115
Corresponding Author: blackwell{at}cbr.med.harvard.edu
The immediate early gene tristetraprolin (TTP) is induced transiently in many cell types by numerous extracellular stimuli. TTP encodes a zinc finger protein that can bind and destabilize mRNAs that encode tumor necrosis factor-alpha (TNFalpha) and other cytokines. We hypothesize that TTP also has a broader role in growth-factor responsive pathways. In support of this model, we have previously determined that TTP induces apoptosis through the mitochondrial pathway, analogously to certain oncogenes and other immediate-early genes, and that TTP sensitizes cells to the pro-apoptotic signals of TNFalpha. In this study, we show that TTP and the related proteins TIS11b and TIS11d bind specifically to 14-3-3 proteins, and that individual 14-3-3 isoforms preferentially bind to different phosphorylated TTP species. 14-3-3 binding does not appear to inhibit or promote induction of apoptosis by TTP, but is one of multiple mechanisms that localize TTP to the cytoplasm. Our results provide the first example of 14-3-3 interacting functionally with an RNA binding protein, and binding in vivo to a Type II 14-3-3 binding site. They also suggest that 14-3-3 binding is part of a complex network of stimuli and interactions that regulate TTP function.
J. Biol. Chem, 10.1074/jbc.M110465200
Submitted on October 31, 2001
Revised on January 30, 2002
Accepted on March 7, 2002
Cytoplasmic localization of Tristetraprolin involves 14-3-3-dependent and –independent mechanisms
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  U. Jalonen, E.-L. Paukkeri, and E. Moilanen Compounds That Increase or Mimic Cyclic Adenosine Monophosphate Enhance Tristetraprolin Degradation in Lipopolysaccharide-Treated Murine J774 Macrophages J. Pharmacol. Exp. Ther., August 1, 2008; 326(2): 514 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. H. Kim, K. Abdelmohsen, A. Lal, R. Pullmann Jr., X. Yang, S. Galban, S. Srikantan, J. L. Martindale, J. Blethrow, K. M. Shokat, et al. Nuclear HuR accumulation through phosphorylation by Cdk1 Genes & Dev., July 1, 2008; 22(13): 1804 - 1815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Pei, P. Zhu, Y. Dang, J. Wu, X. Yang, B. Wan, J. O. Liu, Q. Yi, and L. Yu Nuclear Export of NF90 to Stabilize IL-2 mRNA Is Mediated by AKT-Dependent Phosphorylation at Ser647 in Response to CD28 Costimulation J. Immunol., January 1, 2008; 180(1): 222 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Chang, Y. Fan, S. Karyala, S. Schwemberger, C. R. Tomlinson, M. A. Sartor, and A. Puga Ligand-Independent Regulation of Transforming Growth Factor {beta}1 Expression and Cell Cycle Progression by the Aryl Hydrocarbon Receptor Mol. Cell. Biol., September 1, 2007; 27(17): 6127 - 6139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Sun, G. Stoecklin, S. Van Way, V. Hinkovska-Galcheva, R.-F. Guo, P. Anderson, and T. P. Shanley Tristetraprolin (TTP)-14-3-3 Complex Formation Protects TTP from Dephosphorylation by Protein Phosphatase 2a and Stabilizes Tumor Necrosis Factor-{alpha} mRNA J. Biol. Chem., February 9, 2007; 282(6): 3766 - 3777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Benjamin, M. Schmidlin, L. Min, B. Gross, and C. Moroni BRF1 Protein Turnover and mRNA Decay Activity Are Regulated by Protein Kinase B at the Same Phosphorylation Sites Mol. Cell. Biol., December 15, 2006; 26(24): 9497 - 9507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. I. Gringhuis, J. J. Garcia-Vallejo, B. van het Hof, and W. van Dijk Convergent Actions of I{kappa}B Kinase {beta} and Protein Kinase C{delta} Modulate mRNA Stability through Phosphorylation of 14-3-3{beta} Complexed with Tristetraprolin Mol. Cell. Biol., August 1, 2005; 25(15): 6454 - 6463. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Blackshear, R. S. Phillips, S. Ghosh, S. V.B. Ramos, E. K. Richfield, and W. S. Lai Zfp36l3, a Rodent X Chromosome Gene Encoding a Placenta-Specific Member of the Tristetraprolin Family of CCCH Tandem Zinc Finger Proteins Biol Reprod, August 1, 2005; 73(2): 297 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. F. C. Rigby, K. Roy, J. Collins, S. Rigby, J. E. Connolly, D. B. Bloch, and S. A. Brooks Structure/Function Analysis of Tristetraprolin (TTP): p38 Stress-Activated Protein Kinase and Lipopolysaccharide Stimulation Do Not Alter TTP Function J. Immunol., June 15, 2005; 174(12): 7883 - 7893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lykke-Andersen and E. Wagner Recruitment and activation of mRNA decay enzymes by two ARE-mediated decay activation domains in the proteins TTP and BRF-1 Genes & Dev., February 1, 2005; 19(3): 351 - 361. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Ogilvie, M. Abelson, H. H. Hau, I. Vlasova, P. J. Blackshear, and P. R. Bohjanen Tristetraprolin Down-Regulates IL-2 Gene Expression through AU-Rich Element-Mediated mRNA Decay J. Immunol., January 15, 2005; 174(2): 953 - 961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Arao, A. Kikuchi, M. Kishida, M. Yonekura, A. Inoue, S. Yasuda, S. Wada, K. Ikeda, and F. Kayama Stability of A+U-Rich Element Binding Factor 1 (AUF1)-Binding Messenger Ribonucleic Acid Correlates with the Subcellular Relocalization of AUF1 in the Rat Uterus upon Estrogen Treatment Mol. Endocrinol., September 1, 2004; 18(9): 2255 - 2267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Cao, J. S. Tuttle, and P. J. Blackshear Immunological Characterization of Tristetraprolin as a Low Abundance, Inducible, Stable Cytosolic Protein J. Biol. Chem., May 14, 2004; 279(20): 21489 - 21499. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Chrestensen, M. J. Schroeder, J. Shabanowitz, D. F. Hunt, J. W. Pelo, M. T. Worthington, and T. W. Sturgill MAPKAP Kinase 2 Phosphorylates Tristetraprolin on in Vivo Sites Including Ser178, a Site Required for 14-3-3 Binding J. Biol. Chem., March 12, 2004; 279(11): 10176 - 10184. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Wilson, J. Lu, K. Sutphen, Y. Sun, Y. Huynh, and G. Brewer Regulation of A + U-rich Element-directed mRNA Turnover Involving Reversible Phosphorylation of AUF1 J. Biol. Chem., August 29, 2003; 278(35): 33029 - 33038. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. M. Wilson, J. Lu, K. Sutphen, Y. Suarez, S. Sinha, B. Brewer, E. C. Villanueva-Feliciano, R. M. Ysla, S. Charles, and G. Brewer Phosphorylation of p40AUF1 Regulates Binding to A + U-rich mRNA-destabilizing Elements and Protein-induced Changes in Ribonucleoprotein Structure J. Biol. Chem., August 29, 2003; 278(35): 33039 - 33048. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Worthington, J. W. Pelo, M. A. Sachedina, J. L. Applegate, K. O. Arseneau, and T. T. Pizarro RNA Binding Properties of the AU-rich Element-binding Recombinant Nup475/TIS11/Tristetraprolin Protein J. Biol. Chem., December 6, 2002; 277(50): 48558 - 48564. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
