| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print February 26, 2002
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610-0245
Corresponding Author: mkilberg{at}ufl.edu
The human asparagine synthetase (AS) gene responds to depletion of mammalian cells for either amino acids or carbohydrates. Five specific cis-elements have been implicated: three GC boxes (GC-I, GC-II and GC-III) and two Nutrient Sensing Response Elements (NSRE-1, -2). This study shows that all three GC boxes are required to maintain basal transcription and to obtain maximal induction of the AS gene by amino acid limitation. However, there is not complete redundancy among the three GC boxes and there is a hierarchy of importance with regard to transcription (GC-III > GC-II > GC-I). In vitro, two GC boxes formed protein-DNA complexes (GC-II and GC-III) with Sp1 and Sp3. Although transcription of the AS gene is elevated by nutrient limitation, the absolute amount of these protein-DNA complexes and the total pools of Sp1 and Sp3 did not increase. A small, but detectable portion of Sp1 was modified by phosphorylation following amino acid deprivation. In vivo, expression of Sp1 and Sp3 in Drosophila SL2 cells increased AS promoter activity. Sp1 expression increased basal transcription, but did not cause a further increase when SL2 cells were amino acid deprived. Sp3 expression enhanced both the basal and the starvation-induced transcription.
J. Biol. Chem, 10.1074/jbc.M110972200
Submitted on November 15, 2001
Revised on February 22, 2002
Accepted on February 26, 2002
Role of Sp1 and Sp3 in the nutrient-regulated expression of the human asparagine synthetase gene
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. M. Thiaville, Y.-X. Pan, A. Gjymishka, C. Zhong, R. J. Kaufman, and M. S. Kilberg MEK Signaling Is Required for Phosphorylation of eIF2{alpha} following Amino Acid Limitation of HepG2 Human Hepatoma Cells J. Biol. Chem., April 18, 2008; 283(16): 10848 - 10857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, Y.-X. Pan, E. E. Dudenhausen, and M. S. Kilberg Amino Acid Deprivation Induces the Transcription Rate of the Human Asparagine Synthetase Gene through a Timed Program of Expression and Promoter Binding of Nutrient-responsive Basic Region/Leucine Zipper Transcription Factors as Well as Localized Histone Acetylation J. Biol. Chem., December 3, 2004; 279(49): 50829 - 50839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Scian, K. E. R. Stagliano, M. A. Ellis, S. Hassan, M. Bowman, M. F. Miles, S. P. Deb, and S. Deb Modulation of Gene Expression by Tumor-Derived p53 Mutants Cancer Res., October 15, 2004; 64(20): 7447 - 7454. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Chen, E. E. Dudenhausen, Y.-X. Pan, C. Zhong, and M. S. Kilberg Human CCAAT/Enhancer-binding Protein {beta} Gene Expression Is Activated by Endoplasmic Reticulum Stress through an Unfolded Protein Response Element Downstream of the Protein Coding Sequence J. Biol. Chem., July 2, 2004; 279(27): 27948 - 27956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Palii, H. Chen, and M. S. Kilberg Transcriptional Control of the Human Sodium-coupled Neutral Amino Acid Transporter System A Gene by Amino Acid Availability Is Mediated by an Intronic Element J. Biol. Chem., January 30, 2004; 279(5): 3463 - 3471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Brasse-Lagnel, A. Fairand, A. Lavoinne, and A. Husson Glutamine Stimulates Argininosuccinate Synthetase Gene Expression through Cytosolic O-Glycosylation of Sp1 in Caco-2 Cells J. Biol. Chem., December 26, 2003; 278(52): 52504 - 52510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fernandez, A. B. Lopez, C. Wang, R. Mishra, L. Zhou, I. Yaman, M. D. Snider, and M. Hatzolgou Transcriptional Control of the Arginine/Lysine Transporter, Cat-1, by Physiological Stress J. Biol. Chem., December 12, 2003; 278(50): 50000 - 50009. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Claeyssens, C. Gangneux, C. Brasse-Lagnel, P. Ruminy, T. Aki, A. Lavoinne, and J.-P. Salier Amino acid control of the human glyceraldehyde 3-phosphate dehydrogenase gene transcription in hepatocyte Am J Physiol Gastrointest Liver Physiol, November 1, 2003; 285(5): G840 - G849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Martinez-Chantar, M. U. Latasa, M. Varela-Rey, S. C. Lu, E. R. Garcia-Trevijano, J. M. Mato, and M. A. Avila L-Methionine Availability Regulates Expression of the Methionine Adenosyltransferase 2A Gene in Human Hepatocarcinoma Cells: ROLE OF S-ADENOSYLMETHIONINE J. Biol. Chem., May 23, 2003; 278(22): 19885 - 19890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Bruhat, J. Averous, V. Carraro, C. Zhong, A. M. Reimold, M. S. Kilberg, and P. Fafournoux Differences in the Molecular Mechanisms Involved in the Transcriptional Activation of the CHOP and Asparagine Synthetase Genes in Response to Amino Acid Deprivation or Activation of the Unfolded Protein Response J. Biol. Chem., December 6, 2002; 277(50): 48107 - 48114. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Kilberg and I. P. Barbosa-Tessmann Genomic Sequences Necessary for Transcriptional Activation by Amino Acid Deprivation of Mammalian Cells J. Nutr., July 1, 2002; 132(7): 1801 - 1804. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
