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Papers In Press, published online ahead of print May 7, 2002
Department of Biology, Tokyo Metropolitan University, Hachioji, Tokyo 192-0397
Corresponding Author: komano-teruya{at}c.metro-u.ac.jp
Two genes, fruA and csgA, encoding a putative transcription factor and C-factor, respectively, are essential for fruiting body formation of Myxococcus xanthus. To investigate the role of fruA and csgA genes in developmental gene expression, developing cells as well as vegetative cells of M. xanthus wild-type, fruA::Tc and csgA731 strains were pulse-labeled with [35S]methionine and the whole cell proteins were analyzed using two-dimensional immobilized pH gradient/SDS-PAGE. Differences in protein synthesis patterns among more than 700 protein spots were detected during development of the three strains. Fourteen proteins showing distinctly different expression patterns in mutant cells were analyzed in more detail. Five of the 14 proteins were identified as EF-Tu, Dru, DofA, FruA and protein S by immunoblot analysis and mass spectroscopy. A gene encoding DofA was cloned and sequenced. Although both fruA and csgA genes regulate early development of M. xanthus, they were found to differently regulate expression of several developmental genes. The production of 6 proteins, including DofA and protein S, was dependent on fruA, while the production of 2 proteins was dependent on csgA and 1 protein was dependent on both fruA and csgA. To explain the present findings a new model was presented, in which different levels of FruA phosphorylation may distinctively regulate the expression of two groups of developmental genes.
J. Biol. Chem, 10.1074/jbc.M111214200
Submitted on November 26, 2001
Revised on May 7, 2002
Accepted on May 7, 2002
Role of fruA and csgA genes in gene expression during development of Myxococcus xanthus: Analysis by two-dimensional gel electrophoresis
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