| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print February 26, 2002
Immunologie Virale, Institut Pasteur, 75724, Paris Cedex 15
Corresponding Author: farenzan{at}pasteur.fr
Activation of CXCR4 by the CXC chemokine SDF-1 requires interaction of the amino-terminal domains of both molecules. We report that proteinases released from either mononucleated blood cells or polymorphonuclear neutrophils degranulated by inflammatory stimuli generate a SDF-1 fragment that is deleted from amino-terminal residues Lys1-Pro2-Val3, as characterised by mass spectometry analysis. The proteolysed chemokine fails to induce agonistic functions and is unable to prevent the fusogenic capacity of CXCR4-tropic Human Immunodeficiency Viruses. Furthermore, we observed that exposure of CXCR4-expressing cells to leukocyte proteinases results in the proteolysis of the extracellular amino-terminal domain of the receptor, as assessed by flow cytometry analysis and electrophoretic separation of immunoprecipitated CXCR4. Blockade of SDF-1 and CXCR4 proteolysis by the specific leukocyte elastase inhibitor MeOSuc-Ala-Ala-Pro-Val-CMK, identified elastase as the major enzyme among leukocyte-secreted proteinases that accounts for inactivation of both SDF-1 and CXCR4. Indeed, purified leukocyte elastase generated in either SDF-1 or CXCR4 a pattern of cleavage indistinguishable from that observed with leukocyte-secreted proteinases. Our findings suggest that elastase-mediated proteolysis of SDF-1/CXCR4 is part of a mechanism regulating their biological functions in both homeostatic and pathologic processes.
J. Biol. Chem, 10.1074/jbc.M111388200
Submitted on November 29, 2001
Revised on February 26, 2002
Accepted on February 26, 2002
Leukocyte elastase negatively regulates stromal cell-derived factor-1 (SDF-1)/CXCR4 binding and functions by amino-terminal processing of SDF-1 and CXCR4
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Marquez-Curtis, A. Jalili, K. Deiteren, N. Shirvaikar, A.-M. Lambeir, and A. Janowska-Wieczorek Carboxypeptidase M Expressed by Human Bone Marrow Cells Cleaves the C-Terminal Lysine of Stromal Cell-Derived Factor-1{alpha}: Another Player in Hematopoietic Stem/Progenitor Cell Mobilization? Stem Cells, May 1, 2008; 26(5): 1211 - 1220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-H. Wang, W.-J. Cherng, N.-I Yang, C.-M. Hsu, C.-H. Yeh, Y.-J. Lan, J.-S. Wang, and S. Verma Cyclosporine increases ischemia-induced endothelial progenitor cell mobilization through manipulation of the CD26 system Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2008; 294(3): R811 - R818. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. L. Bristow, R. Wolkowicz, M. Trucy, A. Franklin, F. Di Meo, M. T. Kozlowski, R. Winston, and R. R. Arnold NF-{kappa}B Signaling, Elastase Localization, and Phagocytosis Differ in HIV-1 Permissive and Nonpermissive U937 Clones J. Immunol., January 1, 2008; 180(1): 492 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. De La Luz Sierra, P. Gasperini, P. J. McCormick, J. Zhu, and G. Tosato Transcription factor Gfi-1 induced by G-CSF is a negative regulator of CXCR4 in myeloid cells Blood, October 1, 2007; 110(7): 2276 - 2285. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Xu, J. Sui, H. Tao, Q. Zhu, and W. A. Marasco Human Anti-CXCR4 Antibodies Undergo VH Replacement, Exhibit Functional V-Region Sulfation, and Define CXCR4 Antigenic Heterogeneity J. Immunol., August 15, 2007; 179(4): 2408 - 2418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Donini, S. Fontana, G. Savoldi, W. Vermi, L. Tassone, F. Gentili, E. Zenaro, D. Ferrari, L. D. Notarangelo, F. Porta, et al. G-CSF treatment of severe congenital neutropenia reverses neutropenia but does not correct the underlying functional deficiency of the neutrophil in defending against microorganisms Blood, June 1, 2007; 109(11): 4716 - 4723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Guyon and J.-L. Nahon Multiple actions of the chemokine stromal cell-derived factor-1{alpha} on neuronal activity J. Mol. Endocrinol., March 1, 2007; 38(3): 365 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakayama, N. Mutsuga, and G. Tosato FGF2 posttranscriptionally down-regulates expression of SDF1 in bone marrow stromal cells through FGFR1 IIIc Blood, February 15, 2007; 109(4): 1363 - 1372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Vergote, G. S. Butler, M. Ooms, J. H. Cox, C. Silva, M. D. Hollenberg, J. H. Jhamandas, C. M. Overall, and C. Power Proteolytic processing of SDF-1{alpha} reveals a change in receptor specificity mediating HIV-associated neurodegeneration PNAS, December 12, 2006; 103(50): 19182 - 19187. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kalinkovich, S. Tavor, A. Avigdor, J. Kahn, A. Brill, I. Petit, P. Goichberg, M. Tesio, N. Netzer, E. Naparstek, et al. Functional CXCR4-Expressing Microparticles and SDF-1 Correlate with Circulating Acute Myelogenous Leukemia Cells. Cancer Res., November 15, 2006; 66(22): 11013 - 11020. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. K. Kim, M. De La Luz Sierra, C. K. Williams, A. V. Gulino, and G. Tosato G-CSF down-regulation of CXCR4 expression identified as a mechanism for mobilization of myeloid cells Blood, August 1, 2006; 108(3): 812 - 820. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Sbaa, J. DeWever, P. Martinive, C. Bouzin, F. Frerart, J.-L. Balligand, C. Dessy, and O. Feron Caveolin Plays a Central Role in Endothelial Progenitor Cell Mobilization and Homing in SDF-1-Driven Postischemic Vasculogenesis Circ. Res., May 12, 2006; 98(9): 1219 - 1227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Foudi, P. Jarrier, Y. Zhang, M. Wittner, J.-F. Geay, Y. Lecluse, T. Nagasawa, W. Vainchenker, and F. Louache Reduced retention of radioprotective hematopoietic cells within the bone marrow microenvironment in CXCR4-/- chimeric mice Blood, March 15, 2006; 107(6): 2243 - 2251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Garrood, L. Lee, and C. Pitzalis Molecular mechanisms of cell recruitment to inflammatory sites: general and tissue-specific pathways Rheumatology, March 1, 2006; 45(3): 250 - 260. [Full Text] [PDF]
|
|
|
|
 |
|
 K. Balabanian, B. Lagane, S. Infantino, K. Y. C. Chow, J. Harriague, B. Moepps, F. Arenzana-Seisdedos, M. Thelen, and F. Bachelerie The Chemokine SDF-1/CXCL12 Binds to and Signals through the Orphan Receptor RDC1 in T Lymphocytes J. Biol. Chem., October 21, 2005; 280(42): 35760 - 35766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Lapidot, A. Dar, and O. Kollet How do stem cells find their way home? Blood, September 15, 2005; 106(6): 1901 - 1910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Tavor, I. Petit, S. Porozov, P. Goichberg, A. Avigdor, S. Sagiv, A. Nagler, E. Naparstek, and T. Lapidot Motility, proliferation, and egress to the circulation of human AML cells are elastase dependent in NOD/SCID chimeric mice Blood, September 15, 2005; 106(6): 2120 - 2127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kucia, R. Reca, K. Miekus, J. Wanzeck, W. Wojakowski, A. Janowska-Wieczorek, J. Ratajczak, and M. Z. Ratajczak Trafficking of Normal Stem Cells and Metastasis of Cancer Stem Cells Involve Similar Mechanisms: Pivotal Role of the SDF-1-CXCR4 Axis Stem Cells, August 1, 2005; 23(7): 879 - 894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.-Y. Lozach, L. Burleigh, I. Staropoli, E. Navarro-Sanchez, J. Harriague, J.-L. Virelizier, F. A. Rey, P. Despres, F. Arenzana-Seisdedos, and A. Amara Dendritic Cell-specific Intercellular Adhesion Molecule 3-grabbing Non-integrin (DC-SIGN)-mediated Enhancement of Dengue Virus Infection Is Independent of DC-SIGN Internalization Signals J. Biol. Chem., June 24, 2005; 280(25): 23698 - 23708. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Davis, K. E. Singer, M. De La Luz Sierra, M. Narazaki, F. Yang, H. M. Fales, R. Yarchoan, and G. Tosato Identification of carboxypeptidase N as an enzyme responsible for C-terminal cleavage of stromal cell-derived factor-1{alpha} in the circulation Blood, June 15, 2005; 105(12): 4561 - 4568. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-F. Geay, D. Buet, Y. Zhang, A. Foudi, P. Jarrier, M. Berthebaud, A. G. Turhan, W. Vainchenker, and F. Louache p210BCR-ABL inhibits SDF-1 Chemotactic Response via Alteration of CXCR4 Signaling and Down-regulation of CXCR4 Expression Cancer Res., April 1, 2005; 65(7): 2676 - 2683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Balabanian, B. Lagane, J. L. Pablos, L. Laurent, T. Planchenault, O. Verola, C. Lebbe, D. Kerob, A. Dupuy, O. Hermine, et al. WHIM syndromes with different genetic anomalies are accounted for by impaired CXCR4 desensitization to CXCL12 Blood, March 15, 2005; 105(6): 2449 - 2457. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Selleri, N. Montuori, P. Ricci, V. Visconte, M. V. Carriero, N. Sidenius, B. Serio, F. Blasi, B. Rotoli, G. Rossi, et al. Involvement of the urokinase-type plasminogen activator receptor in hematopoietic stem cell mobilization Blood, March 1, 2005; 105(5): 2198 - 2205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sadir, A. Imberty, F. Baleux, and H. Lortat-Jacob Heparan Sulfate/Heparin Oligosaccharides Protect Stromal Cell-derived Factor-1 (SDF-1)/CXCL12 against Proteolysis Induced by CD26/Dipeptidyl Peptidase IV J. Biol. Chem., October 15, 2004; 279(42): 43854 - 43860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Rao, T. V. Betz, D. J. Lamont, M. B. Kim, S. K. Shaw, R. M. Froio, F. Baleux, F. Arenzana-Seisdedos, R. Alon, and F. W. Luscinskas Elastase Release by Transmigrating Neutrophils Deactivates Endothelial-bound SDF-1{alpha} and Attenuates Subsequent T Lymphocyte Transendothelial Migration J. Exp. Med., September 20, 2004; 200(6): 713 - 724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Gulino, D. Moratto, S. Sozzani, P. Cavadini, K. Otero, L. Tassone, L. Imberti, S. Pirovano, L. D. Notarangelo, R. Soresina, et al. Altered leukocyte response to CXCL12 in patients with warts hypogammaglobulinemia, infections, myelokathexis (WHIM) syndrome Blood, July 15, 2004; 104(2): 444 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. T. Suratt, J. M. Petty, S. K. Young, K. C. Malcolm, J. G. Lieber, J. A. Nick, J.-A. Gonzalo, P. M. Henson, and G. S. Worthen Role of the CXCR4/SDF-1 chemokine axis in circulating neutrophil homeostasis Blood, July 15, 2004; 104(2): 565 - 571. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Hensbergen, D. Verzijl, C. I. A. Balog, R. Dijkman, R. C. van der Schors, E. M. H. van der Raaij-Helmer, M. J. A. van der Plas, R. Leurs, A. M. Deelder, M. J. Smit, et al. Furin Is a Chemokine-modifying Enzyme: IN VITRO AND IN VIVO PROCESSING OF CXCL10 GENERATES A C-TERMINALLY TRUNCATED CHEMOKINE RETAINING FULL ACTIVITY J. Biol. Chem., April 2, 2004; 279(14): 13402 - 13411. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vroon, C. J. Heijnen, R. Raatgever, I. P. Touw, R. E. Ploemacher, R. T. Premont, and A. Kavelaars GRK6 deficiency is associated with enhanced CXCR4-mediated neutrophil chemotaxis in vitro and impaired responsiveness to G-CSF in vivo J. Leukoc. Biol., April 1, 2004; 75(4): 698 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. De La Luz Sierra, F. Yang, M. Narazaki, O. Salvucci, D. Davis, R. Yarchoan, H. H. Zhang, H. Fales, and G. Tosato Differential processing of stromal-derived factor-1{alpha} and stromal-derived factor-1{beta} explains functional diversity Blood, April 1, 2004; 103(7): 2452 - 2459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. E. Ouriaghli, E. Sloand, L. Mainwaring, H. Fujiwara, K. Keyvanfar, J. J. Melenhorst, K. Rezvani, G. Sconocchia, S. Solomon, N. Hensel, et al. Clonal dominance of chronic myelogenous leukemia is associated with diminished sensitivity to the antiproliferative effects of neutrophil elastase Blood, November 15, 2003; 102(10): 3786 - 3792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Villalba, O. Salvucci, Y. Aoki, M. D. L. L. Sierra, G. Gupta, D. Davis, K. Wyvill, R. Little, R. Yarchoan, and G. Tosato Serum inactivation contributes to the failure of stromal-derived factor-1 to block HIV-I infection in vivo J. Leukoc. Biol., November 1, 2003; 74(5): 880 - 888. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. K. Stumm, C. Zhou, T. Ara, F. Lazarini, M. Dubois-Dalcq, T. Nagasawa, V. Hollt, and S. Schulz CXCR4 Regulates Interneuron Migration in the Developing Neocortex J. Neurosci., June 15, 2003; 23(12): 5123 - 5130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Wolf, I. Clark-Lewis, C. Buri, H. Langen, M. Lis, and L. Mazzucchelli Cathepsin D Specifically Cleaves the Chemokines Macrophage Inflammatory Protein-1{alpha}, Macrophage Inflammatory Protein-1{beta}, and SLC That Are Expressed in Human Breast Cancer Am. J. Pathol., April 1, 2003; 162(4): 1183 - 1190. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Kollet, I. Petit, J. Kahn, S. Samira, A. Dar, A. Peled, V. Deutsch, M. Gunetti, W. Piacibello, A. Nagler, et al. Human CD34+CXCR4- sorted cells harbor intracellular CXCR4, which can be functionally expressed and provide NOD/SCID repopulation Blood, September 26, 2002; 100(8): 2778 - 2786. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
