JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on May 24, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/22/19585    most recent
M111451200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cemerski, S.
Right arrow Articles by Romagnoli, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cemerski, S.
Right arrow Articles by Romagnoli, P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print March 26, 2002
J. Biol. Chem, 10.1074/jbc.M111451200
Submitted on November 30, 2001
Revised on March 26, 2002
Accepted on March 26, 2002

Reactive oxygen species differentially affect T cell receptor signaling pathways

Saso Cemerski, Alain Cantagrel, Joost P. M. van Meerwijk, and Paola Romagnoli

Tolerance and autoimmunity section, INSERM, Toulouse 31024

Corresponding Author: Paola.Romagnoli{at}toulouse.inserm.fr

Oxidative stress plays and important role in the induction of T lymphocyte hyporesponsiveness observed in several human pathologies including cancer, rheumatoid arthritis, leprosy and AIDS. To investigate the molecular basis of oxidative stress-induced T cell hyporesponsiveness we have developed an in vitro system in which T lymphocytes are rendered hyporesponsive by co-culture with oxygen radical-producing activated neutrophils. We have observed a direct correlation between the level of T cell hyporesponsiveness induced and the concentration of reactive oxygen species (ROS) produced. Moreover, induction of T cell hyporesponsiveness is blocked by addition of N-acetyl cysteine(NAC),Mn(IIItetrakis(4-benzoic acid)porphyrin chloride (MnTBAP)and catalase confirming the critical role of oxidative stress in this system. The pattern of tyrosine phosphorylated proteins was profoundly altered in hyporesponsive as compared to normal T cells. In hyporesponsive T cells TCR-ligation did no longer induce PLC-g1 activation and caused reduced Ca2+ flux. In contrast, despite increased level of ERK1/2 phosphorylation, TCR-dependent activation of MAP-kinase ERK1/2 was unaltered in hyporesponsive T lymphocytes. A late TCR-signaling event such as caspase 3 activation was as well unaffected in hyporesponsive T lymphocytes. Our data indicate that TCR-signaling pathways are differentially affected by physiological levels of oxidative stress and would suggest that while "hyporesponsive" T cells have lost certain effector functions they may have maintained or gained others.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Immunol.Home page
A. Sinha, A. Singh, V. Satchidanandam, and K. Natarajan
Impaired Generation of Reactive Oxygen Species during Differentiation of Dendritic Cells (DCs) by Mycobacterium tuberculosis Secretory Antigen (MTSA) and Subsequent Activation of MTSA-DCs by Mycobacteria Results in Increased Intracellular Survival
J. Immunol., July 1, 2006; 177(1): 468 - 478.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Vulcano, S. Dusi, D. Lissandrini, R. Badolato, P. Mazzi, E. Riboldi, E. Borroni, A. Calleri, M. Donini, A. Plebani, et al.
Toll Receptor-Mediated Regulation of NADPH Oxidase in Human Dendritic Cells
J. Immunol., November 1, 2004; 173(9): 5749 - 5756.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
G. Nagy, M. Barcza, N. Gonchoroff, P. E. Phillips, and A. Perl
Nitric Oxide-Dependent Mitochondrial Biogenesis Generates Ca2+ Signaling Profile of Lupus T Cells
J. Immunol., September 15, 2004; 173(6): 3676 - 3683.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S. Elsen, J. Doussiere, C. L. Villiers, M. Faure, R. Berthier, A. Papaioannou, N. Grandvaux, P. N. Marche, and P. V. Vignais
Cryptic O2--generating NADPH oxidase in dendritic cells
J. Cell Sci., May 1, 2004; 117(11): 2215 - 2226.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. M. Monick, L. Samavati, N. S. Butler, M. Mohning, L. S. Powers, T. Yarovinsky, D. R. Spitz, and G. W. Hunninghake
Intracellular Thiols Contribute to Th2 Function via a Positive Role in IL-4 Production
J. Immunol., November 15, 2003; 171(10): 5107 - 5115.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
J. Mehrzad, L. Duchateau, S. Pyorala, and C. Burvenich
Blood and Milk Neutrophil Chemiluminescence and Viability in Primiparous and Pluriparous Dairy Cows During Late Pregnancy, Around Parturition and Early Lactation
J Dairy Sci, December 1, 2002; 85(12): 3268 - 3276.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.