| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print January 2, 2002
Department of Pediatrics and Biochemistry, Case Western Reserve University, Cleveland, OH 44106 4948
Corresponding Author: txg2{at}po.cwru.edu
Little is known of the degree that polypeptide sequence and local environment modulate the structures of O-linked glycans. Towards this understanding, the site-specific mono- (
J. Biol. Chem, 10.1074/jbc.M111690200
Submitted on December 7, 2001
Revised on January 2, 2002
Accepted on January 2, 2002
Determination of the site-specific oligosaccharide distribution of the O-glycans attached to the porcine submaxillary mucin tandem repeat: Further evidence for the modulation of O-glycan side chain structures by peptide sequence
-GalNAc-O-), di- (
-Gal (1-3) -GalNAc-O-) and tri- (-Fuc(1-2) -Gal (1-3) -GalNAc-O-) saccharide distribution has been determined for 29 of the 31 O-glycosylated Ser/Thr residues in the tandem repeat domains of blood group A-negative porcine submaxillary gland mucin. The glycosylation patterns obtained from 3 individual animals are in agreement with earlier incomplete determinations on a pooled mucin (J. Biol. Chem. 272, 9709 (1997) & J. Biol Chem. 273, 26580 (1998)), confirming that the addition of the peptide linked GalNAc and its substitution by (1-3) Gal are sensitive to local peptide sequence in a highly reproducible manner in vivo. The present data further support earlier suggestions of an inverse correlation of the density of hydroxyaminoacid residues (and by inference the density of peptide GalNAc) with the extent of substitution of the peptide linked GalNAc by (1-3) Gal. This effect is highly correlated for Ser linked glycans but not for Thr linked glycans. A similar correlation is observed with respect to the in vivo peptide GalNAc glycosylation pattern. In contrast, the addition of -1-2 Fuc to -Gal shows no apparent correlation with hydroxyaminoacid density, although a marked elevation in the fucosylation of Ser linked glycans compared to Thr linked glycans is observed. The above effects may represent both steric and conformational factors acting to alter the relative accessability and activity of the glycosyltransferases towards substrate. These results demonstrate that the porcine submaxillary gland core-1 3-galactosyltransferase and 2-fucosyltransferase exhibit unique peptide/glycopeptide sensitivities which may provide mechanisms for the modulation of O-linked side chain structures.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. B. Maryon, S. A. Molloy, and J. H. Kaplan O-Linked Glycosylation at Threonine 27 Protects the Copper Transporter hCTR1 from Proteolytic Cleavage in Mammalian Cells J. Biol. Chem., July 13, 2007; 282(28): 20376 - 20387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Fritz, J. Raman, and L. A. Tabak Dynamic Association between the Catalytic and Lectin Domains of Human UDP-GalNAc:Polypeptide {alpha}-N-Acetylgalactosaminyltransferase-2 J. Biol. Chem., March 31, 2006; 281(13): 8613 - 8619. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Potter, R. P. Hughey, and O. A. Weisz Role of N- and O-glycans in polarized biosynthetic sorting Am J Physiol Cell Physiol, January 1, 2006; 290(1): C1 - C10. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Freire, S. Bay, S. von Mensdorff-Pouilly, and E. Osinaga Molecular Basis of Incomplete O-Glycan Synthesis in MCF-7 Breast Cancer Cells: Putative Role of MUC6 in Tn Antigen Expression Cancer Res., September 1, 2005; 65(17): 7880 - 7887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. W. Cooling, K. Kelly, J. Barton, D. Hwang, T. A. W. Koerner, and J. D. Olson Determinants of ABH expression on human blood platelets Blood, April 15, 2005; 105(8): 3356 - 3364. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Schultz, K. L. Ferguson, J. Lahnstein, and A. Bacic Post-translational Modifications of Arabinogalactan-peptides of Arabidopsis thaliana: ENDOPLASMIC RETICULUM AND GLYCOSYLPHOSPHATIDYLINOSITOL-ANCHOR SIGNAL CLEAVAGE SITES AND HYDROXYLATION OF PROLINE J. Biol. Chem., October 29, 2004; 279(44): 45503 - 45511. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. G. Ten Hagen, D. T. Tran, T. A. Gerken, D. S. Stein, and Z. Zhang Functional Characterization and Expression Analysis of Members of the UDP-GalNAc:Polypeptide N-Acetylgalactosaminyltransferase Family from Drosophila melanogaster J. Biol. Chem., September 12, 2003; 278(37): 35039 - 35048. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. G. Ten Hagen, T. A. Fritz, and L. A. Tabak All in the family: the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases Glycobiology, January 1, 2003; 13(1): 1R - 16R. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Gerken, J. Zhang, J. Levine, and A. Elhammer Mucin Core O-Glycosylation Is Modulated by Neighboring Residue Glycosylation Status. KINETIC MODELING OF THE SITE-SPECIFIC GLYCOSYLATION OF THE APO-PORCINE SUBMAXILLARY MUCIN TANDEM REPEAT BY UDP-GalNAc:POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASES T1 AND T2 J. Biol. Chem., December 13, 2002; 277(51): 49850 - 49862. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
