JBC INTERFERin siRNA transfection reagent

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on March 15, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/12/10374    most recent
M111799200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Milland, J.
Right arrow Articles by Sandrin, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Milland, J.
Right arrow Articles by Sandrin, M. S.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print January 2, 2002
J. Biol. Chem, 10.1074/jbc.M111799200
Submitted on December 11, 2001
Revised on January 2, 2002
Accepted on January 2, 2002

The cytoplasmic tail of alpha 1,3galactosyltransferase inhibits Golgi localization of the full length enzyme

Julie Milland, Sarah M. Russell, Hayley C. Dodson, Ian F.C. McKenzie, and Mauro S. Sandrin

Molecular Immunogenetics Laboratory, Austin Research Institute, Heidelberg, Victoria 3084

Corresponding Author: m.sandrin{at}ari.unimelb.edu.au

It is currently under debate whether the mechanism of Golgi retention of different glycosyltransferases is determined by sequences in the transmembrane, luminal, or cytoplasmic domains or a combination of these domains. We have shown that the cytoplasmic domains of alpha 1,3galactosyl- (GT) and alpha 1,2fucosyl- (FT) transferases are involved in Golgi localization. Here we show that the cytoplasmic tails of GT and FT are sufficient to confer specific Golgi localization. Further, we show that the expression of only the cytoplasmic tail of GT can lead to displacement or inhibition of binding of the whole transferase and that cells expressing the cytoplasmic tail of GT were not able to express full length GT or its product, Gal alpha (1,3)Gal. Thus, the presence of the cytoplasmic tail prevented the localization and function of full length GT, suggesting a possible specific Golgi binding site for GT. The effect was not altered by the inclusion of the transmembrane domain. Although the transmembrane domain may act as an anchor, these data show that, for GT, only the cytoplasmic tail is involved in specific localization to the Golgi.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
C. Saint-Jore-Dupas, A. Nebenfuhr, A. Boulaflous, M.-L. Follet-Gueye, C. Plasson, C. Hawes, A. Driouich, L. Faye, and V. Gomord
Plant N-Glycan Processing Enzymes Employ Different Targeting Mechanisms for Their Spatial Arrangement along the Secretory Pathway
PLANT CELL, November 1, 2006; 18(11): 3182 - 3200.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. S. Uliana, P. M. Crespo, J. A. Martina, J. L. Daniotti, and H. J. F. Maccioni
Modulation of GalT1 and SialT1 Sub-Golgi Localization by SialT2 Expression Reveals an Organellar Level of Glycolipid Synthesis Control
J. Biol. Chem., October 27, 2006; 281(43): 32852 - 32860.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Schon, C. Prante, C. Bahr, J. Kuhn, K. Kleesiek, and C. Gotting
Cloning and Recombinant Expression of Active Full-length Xylosyltransferase I (XT-I) and Characterization of Subcellular Localization of XT-I and XT-II
J. Biol. Chem., May 19, 2006; 281(20): 14224 - 14231.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Milland, D. Christiansen, B. D. Lazarus, S. G. Taylor, P. X. Xing, and M. S. Sandrin
The Molecular Basis for Gal{alpha}(1,3)Gal Expression in Animals with a Deletion of the {alpha}1,3Galactosyltransferase Gene
J. Immunol., February 15, 2006; 176(4): 2448 - 2454.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. H. Fenteany and K. J. Colley
Multiple Signals Are Required for {alpha}2,6-Sialyltransferase (ST6Gal I) Oligomerization and Golgi Localization
J. Biol. Chem., February 18, 2005; 280(7): 5423 - 5429.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
N. Nagai, H. Habuchi, J. D. Esko, and K. Kimata
Stem domains of heparan sulfate 6-O-sulfotransferase are required for Golgi localization, oligomer formation and enzyme activity
J. Cell Sci., July 1, 2004; 117(15): 3331 - 3341.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Crimeen-Irwin, S. Ellis, D. Christiansen, M. J. Ludford-Menting, J. Milland, M. Lanteri, B. E. Loveland, D. Gerlier, and S. M. Russell
Ligand Binding Determines Whether CD46 Is Internalized by Clathrin-coated Pits or Macropinocytosis
J. Biol. Chem., November 21, 2003; 278(47): 46927 - 46937.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. L. Sousa, C. Brito, T. Costa, J. Lanoix, T. Nilsson, and J. Costa
Importance of Cys, Gln, and Tyr from the Transmembrane Domain of Human alpha 3/4 Fucosyltransferase III for Its Localization and Sorting in the Golgi of Baby Hamster Kidney Cells
J. Biol. Chem., February 21, 2003; 278(9): 7624 - 7629.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.