JBC Advanced Peptides, Inc.

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on May 3, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/18/16189    most recent
M111936200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Tang, S.
Right arrow Articles by Tang, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tang, S.
Right arrow Articles by Tang, D. G.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print February 11, 2002
J. Biol. Chem, 10.1074/jbc.M111936200
Submitted on December 14, 2001
Revised on January 22, 2002
Accepted on February 11, 2002

Evidence that Arachidonate 15-lipoxygenase 2 Is a negative cell-cycle regulator in normal prostate epithelial cells

Shaohua Tang, Bobby Bhatia, Carlos J. Maldonado, Peiying Yang, Robert A. Newman, Junwei Liu, Dhyan Chandra, Jeanine Traag, Russell D Klein, Susan M. Fischer, Dharam Chopra, Jianjun Shen, Haiyen Zhau, Leland W.K. Chung, and Dean G. Tang

Carcinogenesis, University of Texas, Smithville, TX 78957

Corresponding Author: dtang{at}sprd1.mdacc.tmc.edu

15-Lipoxygenase 2 (15-LOX2) is a recently cloned human lipoxygenase that shows tissue-restricted expression in prostate, lung, skin, and cornea. The protein level and enzymatic activity of 15-LOX2 have been shown to be downregulated in prostate cancers compared with normal and benign prostate tissues (Shappell et al., Am. J. Pathol. 155, 235-245, 1999). The biological function of 15-LOX2 and the role of loss of 15-LOX2 expression in prostate tumorigenesis, however, remain unknown. Here we report the cloning and functional characterization of 15-LOX2 and its three splice variants (termed 15-LOX2sv-a, 15-LOX2sv-b, and 15-LOX2sv-c) from primary prostate epithelial cells. Western blotting with multiple primary prostate cell strains and prostate cancer cell lines reveal that the expression of 15-LOX2 is lost in all prostate cancer cell lines, accompanied by decreased enzymatic activity revealed by LC/MSMS analyses. Further experiments show that the loss of 15-LOX2 expression results from transcriptional repression caused by mechanism(s) other than promoter hypermethylation or histone deacetylation. Subsequent functional studies indicate that: 1) the 15-LOX2 product, 15(S)-hydroxyeicosatetraenoic acid [15(S)-HETE], inhibits prostate cancer cell-cycle progression; 2) 15-LOX2 expression in primary prostate epithelial cells is inversely correlated with cell cycle; and 3) restoration of 15-LOX2 expression in prostate cancer cells partially inhibits cell-cycle progression. Taken together, these results suggest that 15-LOX2 could be a suppressor of prostate cancer development, which functions by restricting cell-cycle progression.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
B. Bhatia, M. Jiang, M. Suraneni, L. Patrawala, M. Badeaux, R. Schneider-Broussard, A. S. Multani, C. R. Jeter, T. Calhoun-Davis, L. Hu, et al.
Critical and Distinct Roles of p16 and Telomerase in Regulating the Proliferative Life Span of Normal Human Prostate Epithelial Progenitor Cells
J. Biol. Chem., October 10, 2008; 283(41): 27957 - 27972.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
E. J. Chapman, G. Kelly, and M. A. Knowles
Genes Involved in Differentiation, Stem Cell Renewal, and Tumorigenesis Are Modulated in Telomerase-Immortalized Human Urothelial Cells
Mol. Cancer Res., July 1, 2008; 6(7): 1154 - 1168.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
D. Schweiger, G. Furstenberger, and P. Krieg
Inducible expression of 15-lipoxygenase-2 and 8-lipoxygenase inhibits cell growth via common signaling pathways
J. Lipid Res., March 1, 2007; 48(3): 553 - 564.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Chen, S. Tsui, W. E. Boeglin, R. S. Douglas, A. R. Brash, and T. J. Smith
Interleukin-4 Induces 15-Lipoxygenase-1 Expression in Human Orbital Fibroblasts from Patients with Graves Disease: EVIDENCE FOR ANATOMIC SITE-SELECTIVE ACTIONS OF Th2 CYTOKINES
J. Biol. Chem., July 7, 2006; 281(27): 18296 - 18306.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
D M Peehl
Primary cell cultures as models of prostate cancer development
Endocr. Relat. Cancer, March 1, 2005; 12(1): 19 - 47.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
M. S. Chang, C. Schneider, R. L. Roberts, S. B. Shappell, F. R. Haselton, W. E. Boeglin, and A. R. Brash
Detection and Subcellular Localization of Two 15S-Lipoxygenases in Human Cornea
Invest. Ophthalmol. Vis. Sci., March 1, 2005; 46(3): 849 - 856.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
V. Subbarayan, A. L. Sabichi, J. Kim, N. Llansa, C. J. Logothetis, S. M. Lippman, and D. G. Menter
Differential Peroxisome Proliferator-Activated Receptor-{gamma} Isoform Expression and Agonist Effects in Normal and Malignant Prostate Cells
Cancer Epidemiol. Biomarkers Prev., November 1, 2004; 13(11): 1710 - 1716.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
E. K. Rydberg, A. Krettek, C. Ullstrom, K. Ekstrom, P.-A. Svensson, L. M.S. Carlsson, A.-C. Jonsson-Rylander, G. I. Hansson, W. McPheat, O. Wiklund, et al.
Hypoxia Increases LDL Oxidation and Expression of 15-Lipoxygenase-2 in Human Macrophages
Arterioscler. Thromb. Vasc. Biol., November 1, 2004; 24(11): 2040 - 2045.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Clin. Nutr.Home page
S. C Larsson, M. Kumlin, M. Ingelman-Sundberg, and A. Wolk
Dietary long-chain n-3 fatty acids for the prevention of cancer: a review of potential mechanisms
Am. J. Clinical Nutrition, June 1, 2004; 79(6): 935 - 945.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. Bhatia, C. J. Maldonado, S. Tang, D. Chandra, R. D. Klein, D. Chopra, S. B. Shappell, P. Yang, R. A. Newman, and D. G. Tang
Subcellular Localization and Tumor-suppressive Functions of 15-Lipoxygenase 2 (15-LOX2) and Its Splice Variants
J. Biol. Chem., June 27, 2003; 278(27): 25091 - 25100.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. B. Shappell, S. J. Olson, S. E. Hannah, S. Manning, R. L. Roberts, N. Masumori, M. Jisaka, W. E. Boeglin, V. Vader, D. S. Dave, et al.
Elevated Expression of 12/15-Lipoxygenase and Cyclooxygenase-2 in a Transgenic Mouse Model of Prostate Carcinoma
Cancer Res., May 1, 2003; 63(9): 2256 - 2267.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. C. Hsi, L. C. Wilson, and T. E. Eling
Opposing Effects of 15-Lipoxygenase-1 and -2 Metabolites on MAPK Signaling in Prostate. ALTERATION IN PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR gamma
J. Biol. Chem., October 18, 2002; 277(43): 40549 - 40556.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.