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Papers In Press, published online ahead of print November 11, 2002
J. Biol. Chem, 10.1074/jbc.M112180200
Submitted on December 20, 2001
Revised on November 11, 2002
Accepted on November 11, 2002

Whole genome expression profiles of yeast RNA polymerase II core subunit, Rpb4, in stress and non-stress conditions

Beena Pillai, Jiyoti Verma, Anju Abraham, Princy Francis, Yadunanda Kumar, Utpal Tatu, Samir K. Bramhachari, and Parag P. Sadhale

Microbiology and Cell Biology, Indian Institute of Science, Bangalore, Karnataka 560 012

Corresponding Author: pps{at}mcbl.iisc.ernet.in

Organisms respond to environmental stress by adopting changes in gene expression at the transcriptional level. Rpb4, a non-essential subunit of the core RNA polymerase II has been proposed to play a role in non-stress specific transcription and in regulation of stress response in yeast. We find that besides the temperature sensitivity of the null mutant of Rpb4, diploid null mutants are also compromised in sporulation and show morphological changes associated with nitrogen starvation. Using whole genome expression analysis, we report here, the effects of Rpb4 on expression of genes during normal growth and following heat shock and nutritional starvation. Our analysis shows that Rpb4 affects expression of a small yet significant fraction of the genome in both stress and normal conditions. We found that genes involved in galactose metabolism were dependent on presence of Rpb4 irrespective of the environmental condition. Rpb4 was also found to affect the expression of several other genes specifically in conditions of nutritional starvation. The general defect in the absence of Rpb4 is in the expression of metabolic genes especially those involved in carbon metabolism and energy generation. We report that various stresses are affected by RPB4 and that on over expression the stress specific activators can partially rescue the corresponding defects.


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