| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print February 28, 2002
Biochemistry, Sasaki Institute, Tokyo 101-0062
Corresponding Author: yamashita{at}sasaki.or.jp
VIP36, an intracellular lectin that recognizes high-mannose type glycans (Hara-Kuge, et al. (1999) Glycobiology 9, 833-839), was shown to localize not only to the early secretory pathway but also to plasma membrane of MDCK cells. In the plasma membrane, VIP36 exhibited an apical-predominant distribution, the apical/basolateral ratio being ~2. Like VIP36, plasma membrane glycoproteins recognized by VIP36 were found in the apical and basolateral membranes in the ratio of ~2 to 1. In addition, secretory glycoproteins recognized by VIP36 were secreted ~2-fold more efficiently from the apical membrane than from the basolateral membrane. Thus, the apical/basolateral ratio of the transport of VIP36-recognized glycoproteins was correlated with that of VIP36 in MDCK cells. Upon overproduction of VIP36 in MDCK cells, the apical/basolateral ratios of both VIP36 and VIP36-recognized glycoproteins were changed from ~2 to ~4, and the secretion of VIP36-recognized glycoproteins was greatly stimulated. In contrast to the overproduction of VIP36, that of a mutant version of VIP36, which has no lectin activity, was of no effect on the distribution of glycoproteins to apical and basolateral membranes and inhibited the secretion of VIP36-recognized glycoproteins. Furthermore, the overproduction of VIP36 greatly stimulated the secretion of a major apical secretory glycoprotein of MDCK cells, clusterin, which was found to carry at least one high-mannose type glycan and be recognized by VIP36. In contrast to the secretion of clusterin, that of a none-glycosylated apical-secretion protein, galectin-3, was not stimulated through the overproduction of VIP36. These results indicated that VIP36 was involved in the transport and sorting of glycoproteins carrying high mannose type glycan(s).
J. Biol. Chem, 10.1074/jbc.M112188200
Submitted on December 20, 2001
Revised on February 25, 2002
Accepted on February 28, 2002
Involvement of VIP36 in intracellular transport and secretion of glycoproteins in polarized MDCK cells
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Cao, C. Li, J. N. Higginbotham, J. L. Franklin, D. L. Tabb, R. Graves-Deal, S. Hill, K. Cheek, W. G. Jerome, L. A. Lapierre, et al. Use of Fluorescence-activated Vesicle Sorting for Isolation of Naked2-associated, Basolaterally Targeted Exocytic Vesicles for Proteomics Analysis Mol. Cell. Proteomics, September 1, 2008; 7(9): 1651 - 1667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Halbleib, A. M. Saaf, P. O. Brown, and W. J. Nelson Transcriptional Modulation of Genes Encoding Structural Characteristics of Differentiating Enterocytes During Development of a Polarized Epithelium In Vitro Mol. Biol. Cell, November 1, 2007; 18(11): 4261 - 4278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Satoh, N. P. Cowieson, W. Hakamata, H. Ideo, K. Fukushima, M. Kurihara, R. Kato, K. Yamashita, and S. Wakatsuki Structural Basis for Recognition of High Mannose Type Glycoproteins by Mammalian Transport Lectin VIP36 J. Biol. Chem., September 21, 2007; 282(38): 28246 - 28255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Nawa, O. Shimada, N. Kawasaki, N. Matsumoto, and K. Yamamoto Stable interaction of the cargo receptor VIP36 with molecular chaperone BiP Glycobiology, September 1, 2007; 17(9): 913 - 921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Kawasaki, I. Matsuo, K. Totani, D. Nawa, N. Suzuki, D. Yamaguchi, N. Matsumoto, Y. Ito, and K. Yamamoto Detection of Weak Sugar Binding Activity of VIP36 using VIP36-streptavidin Complex and Membrane-based Sugar Chains J. Biochem., February 1, 2007; 141(2): 221 - 229. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Shang, X. W. Zheng, M. Niiya, and X. L. Zheng Apical sorting of ADAMTS13 in vascular endothelial cells and Madin-Darby canine kidney cells depends on the CUB domains and their association with lipid rafts Blood, October 1, 2006; 108(7): 2207 - 2215. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Potter, R. P. Hughey, and O. A. Weisz Role of N- and O-glycans in polarized biosynthetic sorting Am J Physiol Cell Physiol, January 1, 2006; 290(1): C1 - C10. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Vagin, S. Turdikulova, and G. Sachs Recombinant Addition of N-Glycosylation Sites to the Basolateral Na,K-ATPase beta1 Subunit Results in Its Clustering in Caveolae and Apical Sorting in HGT-1 Cells J. Biol. Chem., December 30, 2005; 280(52): 43159 - 43167. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kamiya, Y. Yamaguchi, N. Takahashi, Y. Arata, K.-i. Kasai, Y. Ihara, I. Matsuo, Y. Ito, K. Yamamoto, and K. Kato Sugar-binding Properties of VIP36, an Intracellular Animal Lectin Operating as a Cargo Receptor J. Biol. Chem., November 4, 2005; 280(44): 37178 - 37182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. P. Mishin, D. Novikov, F. G. Hayden, and L. V. Gubareva Effect of Hemagglutinin Glycosylation on Influenza Virus Susceptibility to Neuraminidase Inhibitors J. Virol., October 1, 2005; 79(19): 12416 - 12424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Breuza, R. Halbeisen, P. Jeno, S. Otte, C. Barlowe, W. Hong, and H.-P. Hauri Proteomics of Endoplasmic Reticulum-Golgi Intermediate Compartment (ERGIC) Membranes from Brefeldin A-treated HepG2 Cells Identifies ERGIC-32, a New Cycling Protein That Interacts with Human Erv46 J. Biol. Chem., November 5, 2004; 279(45): 47242 - 47253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pang, P. Urquhart, and N. M. Hooper N-Glycans, not the GPI anchor, mediate the apical targeting of a naturally glycosylated, GPI-anchored protein in polarised epithelial cells J. Cell Sci., October 1, 2004; 117(21): 5079 - 5086. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hara-Kuge, A. Seko, O. Shimada, H. Tosaka-Shimada, and K. Yamashita The binding of VIP36 and {alpha}-amylase in the secretory vesicles via high-mannose type glycans Glycobiology, August 1, 2004; 14(8): 739 - 744. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Potter, G. Ihrke, J. R. Bruns, K. M. Weixel, and O. A. Weisz Specific N-Glycans Direct Apical Delivery of Transmembrane, but Not Soluble or Glycosylphosphatidylinositol-anchored Forms of Endolyn in Madin-Darby Canine Kidney Cells Mol. Biol. Cell, March 1, 2004; 15(3): 1407 - 1416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. S. Trombetta The contribution of N-glycans and their processing in the endoplasmic reticulum to glycoprotein biosynthesis Glycobiology, September 1, 2003; 13(9): 77R - 91R. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Shimada, S. Hara-Kuge, K. Yamashita, H. Tosaka-Shimada, L. Yanchao, L. Einan, S. Atsumi, and H. Ishikawa Localization of VIP36 in the Post-Golgi Secretory Pathway Also of Rat Parotid Acinar Cells J. Histochem. Cytochem., August 1, 2003; 51(8): 1057 - 1063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Hoekstra, O. Maier, J. M. van der Wouden, T. A. Slimane, and S. C. D. van IJzendoorn Membrane dynamics and cell polarity: the role of sphingolipids J. Lipid Res., May 1, 2003; 44(5): 869 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Nufer, S. Mitrovic, and H.-P. Hauri Profile-based Data Base Scanning for Animal L-type Lectins and Characterization of VIPL, a Novel VIP36-like Endoplasmic Reticulum Protein J. Biol. Chem., April 25, 2003; 278(18): 15886 - 15896. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
