| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print February 19, 2002
J. Biol. Chem, 10.1074/jbc.M112266200
Submitted on December 21, 2001
Revised on February 8, 2002
Accepted on February 19, 2002
Molecular Pathogenesis and Pathology, Skirball Institute, NYU School of Medicine, New York, NY 10016
Corresponding Author: smithsu{at}saturn.med.nyu.edu
Tankyrase1, a human telomeric poly(ADP-ribose) polymerase (PARP), was originally identified through its interaction with TRF1, a negative regulator of telomere length. Tankyrase1 ADP-ribosylates TRF1 in vitro and its overexpression induces telomere elongation in human cancer cells. In addition to its telomeric localization, tankyrase1 resides at multiple subcellular sites, suggesting additional functions for this protein. Here we identify TAB182, a novel tankyrase1-binding protein of 182 kD. TAB182 displays a complex pattern of subcellular localization. TAB182 localizes to the nucleus in a heterochromatic staining pattern and to the cytoplasm, where it costains with the cortical actin network. TAB182 coimmunoprecipitates with tankyrase1 from human cells and serves as an acceptor of poly(ADP-ribosyl)ation by tankyrase1 in vitro. Like TRF1, TAB182 binds to the ankyrin domain (comprising 24 ankyrin repeats) of tankyrase1. Surprisingly, dissection of this domain reveals multiple, discrete and overlapping, binding sites for TRF1 and TAB182. Thus, we demonstrate five well conserved ANK repeat clusters (ARCs) in tankyrase1. While each of the five ARCs independently binds to TRF1, only three of the five bind toTAB182. These findings suggest that tankyrase1 may act as a scaffold for large molecular weight complexes made up of multiple binding proteins. We discuss potential roles for tankyrase1-mediated higher order complexes at telomeres and at other subcellular sites.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Morita, T. Suzuki, T. Nakamura, K. Yokoyama, T. Miyasaka, and T. Yamamoto Depletion of Mammalian CCR4b Deadenylase Triggers Elevation of the p27Kip1 mRNA Level and Impairs Cell Growth Mol. Cell. Biol., July 1, 2007; 27(13): 4980 - 4990. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Hsiao, M. F. Poitras, B. D. Cook, Y. Liu, and S. Smith Tankyrase 2 Poly(ADP-Ribose) Polymerase Domain-Deleted Mice Exhibit Growth Defects but Have Normal Telomere Length and Capping. Mol. Cell. Biol., March 1, 2006; 26(6): 2044 - 2054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Deng, C. Atanasiu, K. Zhao, R. Marmorstein, J. I. Sbodio, N.-W. Chi, and P. M. Lieberman Inhibition of Epstein-Barr Virus OriP Function by Tankyrase, a Telomere-Associated Poly-ADP Ribose Polymerase That Binds and Modifies EBNA1 J. Virol., April 15, 2005; 79(8): 4640 - 4650. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. De Rycker and C. M. Price Tankyrase Polymerization Is Controlled by Its Sterile Alpha Motif and Poly(ADP-Ribose) Polymerase Domains Mol. Cell. Biol., November 15, 2004; 24(22): 9802 - 9812. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. O'Connor, A. Safari, D. Liu, J. Qin, and Z. Songyang The Human Rap1 Protein Complex and Modulation of Telomere Length J. Biol. Chem., July 2, 2004; 279(27): 28585 - 28591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. K. Mosavi, T. J. Cammett, D. C. Desrosiers, and Z.-y. Peng The ankyrin repeat as molecular architecture for protein recognition Protein Sci., June 1, 2004; 13(6): 1435 - 1448. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Dynek and S. Smith Resolution of Sister Telomere Association Is Required for Progression Through Mitosis Science, April 2, 2004; 304(5667): 97 - 100. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Seimiya, Y. Muramatsu, S. Smith, and T. Tsuruo Functional Subdomain in the Ankyrin Domain of Tankyrase 1 Required for Poly(ADP-Ribosyl)ation of TRF1 and Telomere Elongation Mol. Cell. Biol., March 1, 2004; 24(5): 1944 - 1955. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wei and C. M. Price Cell Cycle Localization, Dimerization, and Binding Domain Architecture of the Telomere Protein cPot1 Mol. Cell. Biol., March 1, 2004; 24(5): 2091 - 2102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Chang, J. N. Dynek, and S. Smith TRF1 is degraded by ubiquitin-mediated proteolysis after release from telomeres Genes & Dev., June 1, 2003; 17(11): 1328 - 1333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bae, J. R. Donigian, and A. J. W. Hsueh Tankyrase 1 Interacts with Mcl-1 Proteins and Inhibits Their Regulation of Apoptosis J. Biol. Chem., February 7, 2003; 278(7): 5195 - 5204. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Virag and C. Szabo The Therapeutic Potential of Poly(ADP-Ribose) Polymerase Inhibitors Pharmacol. Rev., September 1, 2002; 54(3): 375 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. I. Sbodio and N.-W. Chi Identification of a Tankyrase-binding Motif Shared by IRAP, TAB182, and Human TRF1 but Not Mouse TRF1. NuMA CONTAINS THIS RXXPDG MOTIF AND IS A NOVEL TANKYRASE PARTNER J. Biol. Chem., August 23, 2002; 277(35): 31887 - 31892. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
