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Papers In Press, published online ahead of print February 12, 2002
Department of Medical Biochemistry, Göteborg University, Gothenburg 405 30
Corresponding Author: gunnar.hansson{at}medkem.gu.se
Neutral mucin oligosaccharides from the small intestine of control rats and rats infected with the parasite Nippostrongylus brasiliensis were released and analyzed by gas chromatography-mass spectrometry. Infected animals expressed seven blood group A-like structures that all were absent in the control animals. The blood group A nature of these epitopes was confirmed by blood group A reactivity of the prepared mucins of which Muc2 was one. Transferase assays and northern blotting on small intestines from infected animals showed that an a-N-acetylgalactosaminyltransferase similar to the human blood group A glycosyltransferase had been induced. The expression was a transient event, with a maximum at day six of the thirteen days long infection. The rat blood group A glycosyltransferase was cloned, revealing two forms with an amino acid similarity of 95 percent. Both types had blood group A transferase activity and were probably allelic as none of twelve analyzed inbred strains carried both types. The second type was found in outbred rats and in one inbred strain. First generation offsprings of inbred rats of each type were heterozygous, further supporting the allelic hypothesis. The transient induction and the large allelic variation could suggest that glycosyltransferases are part of a dynamic system altering mucins and other glycoconjugates as a protecting mechanism against microbial challenges.
J. Biol. Chem, 10.1074/jbc.M112287200
Submitted on December 21, 2001
Revised on February 7, 2002
Accepted on February 12, 2002
Blood group A glycosyltransferase occurring as Alleles with high sequence difference is transiently induced during a Nippostrongylus Brasiliensis parasite infection
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