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Papers In Press, published online ahead of print March 1, 2002
Medicinal Chemistry & Molecular Pharmacology, Purdue University, West Lafayette, IN 47907-1333
Corresponding Author: morre{at}pharmacy.purdue.edu
Plasma membrane associated redox systems play important roles in regulation of cell growth, internal pH, signal transduction, apoptosis and defense against pathogens. Stimulation of cell growth and stimulation of the redox system of plasma membranes are correlated. When cell growth is inhibited by antitumor agents such as doxorubicin, capsaicin and antitumor sulfonylureas, redox activities of the plasma membrane also are inhibited. A doxorubicin-inhibited NADH quinone reductase was characterized and purified from plasma membranes of rat liver. First, an NADH-cytochrome b5 reductase, which was doxorubicin-insensitive, was removed from the plasma membranes by the lysosomal protease, cathepsin D. After removal of the NADH-cytochrome b5 reductase, the plasma membranes retained a doxorubicin-inhibited NADH quinone reductase activity. The enzyme, with an apparent molecular weight of 57 kD, was purified 200-fold over the cathepsin D-treated plasma membranes. The purified enzyme had also an NADH-coenzyme Q0 (CoQ0) reductase [NADH: External Acceptor (Quinone) Reductase 1.6.99. .)] activity. Partial amino acid sequence of the enzyme showed that it was unique with no sequence homology to any known protein. Antibody against the enzyme (peptide sequence) was produced and affinity purified. The purified antibody immunoprecipitated both the NADH-ferricyanide reductase activity and NADH-CoQ0 reductase activity of plasma membranes and cross-reacted with the human chronic myelogenous leukemia cell (K562) cells and doxorubicin-resistant human chronic myelogenous leukemia cell (K562R) cells. Localization by fluorescence microscopy showed that the reaction was with the external surface of the plasma membranes. The doxorubicin-inhibited NADH quinone reductase may provide a target for the anthracycline antitumor agents.
J. Biol. Chem, 10.1074/jbc.M112311200
Submitted on December 21, 2001
Revised on February 11, 2002
Accepted on March 1, 2002
Purification and characterization of a doxorubicin-inhibited NADH-quinone (NADH-ferricyanide) reductase from rat liver plasma membranes
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