| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print February 6, 2002
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021
Corresponding Author: k-marians{at}ski.mskcc.org
Recombination-dependent replication is an essential housekeeping function in prokaryotes and eukaryotes, serving, for example, to restart DNA replication after the repair of a double-strand break. Little is known about the interplay between the recombination and replication machinery when recombination intermediates are used as substrates for DNA replication. We show here that recombination intermediates formed between linear duplex and supercoiled plasmid DNAs are substrates for a generalized strand displacement DNA synthesis reaction where the 3¢-OH of the invading strand in the recombination intermediate is used as a primer. DNA synthesis is driven by negative superhelicity and is inhibited if disassembly of the RecA filament is prevented. Thus, assembly and disassembly of RecA filaments in the same direction facilitates filament clearance from the 3¢-end of the invading strand, allowing DNA synthesis to occur from recombination intermediates.
J. Biol. Chem, 10.1074/jbc.M112418200
Submitted on December 27, 2001
Revised on February 6, 2002
Accepted on February 5, 2002
A dynamic RecA filament permits DNA polymerase-catalyzed extension of the invading strand in recombination intermediates
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  L. S. Symington and W.-D. Heyer Some disassembly required: role of DNA translocases in the disruption of recombination intermediates and dead-end complexes Genes & Dev., September 15, 2006; 20(18): 2479 - 2486. [Full Text] [PDF]
|
|
|
|
 |
|
 W.-D. Heyer, X. Li, M. Rolfsmeier, and X.-P. Zhang Rad54: the Swiss Army knife of homologous recombination? Nucleic Acids Res., September 10, 2006; 34(15): 4115 - 4125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ristic, M. Modesti, T. van der Heijden, J. van Noort, C. Dekker, R. Kanaar, and C. Wyman Human Rad51 filaments on double- and single-stranded DNA: correlating regular and irregular forms with recombination function Nucleic Acids Res., June 8, 2005; 33(10): 3292 - 3302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Fulconis, A. Bancaud, J.-F. Allemand, V. Croquette, M. Dutreix, and J.-L. Viovy Twisting and Untwisting a Single DNA Molecule Covered by RecA Protein Biophys. J., October 1, 2004; 87(4): 2552 - 2563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Nimonkar and P. E. Boehmer Role of Protein-Protein Interactions during Herpes Simplex Virus Type 1 Recombination-dependent Replication J. Biol. Chem., May 21, 2004; 279(21): 21957 - 21965. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Nimonkar and P. E. Boehmer Reconstitution of recombination-dependent DNA synthesis in herpes simplex virus 1 PNAS, September 2, 2003; 100(18): 10201 - 10206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Nimonkar and P. E. Boehmer The Herpes Simplex Virus Type-1 Single-strand DNA-binding Protein (ICP8) Promotes Strand Invasion J. Biol. Chem., March 7, 2003; 278(11): 9678 - 9682. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Nurse, C. Levine, H. Hassing, and K. J. Marians Topoisomerase III Can Serve as the Cellular Decatenase in Escherichia coli J. Biol. Chem., February 28, 2003; 278(10): 8653 - 8660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ayora, R. Missich, P. Mesa, R. Lurz, S. Yang, E. H. Egelman, and J. C. Alonso Homologous-pairing Activity of the Bacillus subtilis Bacteriophage SPP1 Replication Protein G35P J. Biol. Chem., September 20, 2002; 277(39): 35969 - 35979. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
