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Papers In Press, published online ahead of print February 11, 2002
J. Biol. Chem, 10.1074/jbc.M200316200
Submitted on January 11, 2002
Revised on February 11, 2002
Accepted on February 10, 2002

Influences of a guanine-derived formamidopyrimidine lesion on DNA replication. Translesion DNA synthesis, nucleotide insertion and exension kinetics

Kenjiro Asagoshi, Hiroaki Terato, Yoshihiko Ohyama, and Hiroshi Ide

Department of Mathematical and Life Sciences, Hiroshima University, Graduate School of Science, Higashi-Hiroshima 739-8526

Corresponding Author: ideh{at}hiroshima-u.ac.jp

2,6-Diamino-4-hydroxy-5-formamidopyrimidine (FapyG) is a major guanine lesion formed by reactive oxygen species. In this study, a defined oligonucleotide template containing a 5-N-methylated analogue of FapyG (mFapyG) was prepared and its influence on DNA replication was quantitatively assessed in vitro. The results were further compared to those obtained for 7,8-dihydro-8-oxoguanine (8oxoG) and an apurinic/apyrimidinic (AP) site embedded in the same sequence context. mFapyG constituted a fairly strong but not absolute block to DNA synthesis catalyzed by Escherichia coli DNA polymerase I Klenow fragment proficient and deficient in 3’-5’ exonuclease, thereby permitting translesion synthesis with a limited efficiency. The efficiency of translesion synthesis was G > 8oxoG > mFapyG > AP site. Analysis of the nucleotide insertion (fins = Vmax/Km for insertion) and extension efficiencies (fext = Vmax/Km for extension) for mFapyG revealed that the extension step constituted a major kinetic barrier to DNA synthesis. When mFapyG was bypassed, dCMP, a cognate nucleotide, was preferentially inserted opposite the lesion (dCMP (relative fins = 1) >> dTMP (2.4 x 10-4) >= dAMP (8.1 x 10-5) > dGMP (4.5 x 10-7)) and the primer terminus containing a mFapyG:C pair was most efficiently extended (mFapyG:C (relative fext = 1) > mFapyG:T (4.6 x 10-3) >> mFapyG:A and mFapyG:G (extension not observed)). Thus, mFapyG is a potentially lethal but not premutagenic lesion.


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