| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print April 11, 2002
Dept. of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720
Corresponding Author: schekman{at}uclink4.berkeley.edu
The yeast plasma membrane H+-ATPase, Pma1p, is one of the most abundant proteins to traverse the secretory pathway. Newly synthesized Pma1p exits the endoplasmic reticulum (ER) via COPII-coated vesicles bound for the Golgi. Unlike most secreted proteins, efficient incorporation of Pma1p into COPII vesicles requires the Sec24p homolog, Lst1p, suggesting a unique role for Lst1p in ER export. Vesicles formed with mixed Sec24p/Lst1p coats are larger than those with Sec24p alone. Here we examine the relationship between Pma1p biosynthesis and the requirement for this novel coat subunit. We show that Pma1p forms a large oligomeric complex of >1 MDa in the ER, which is packaged into COPII vesicles. Furthermore, oligomerization of Pma1p is linked to membrane lipid composition; Pma1p is rendered monomeric in cells depleted of ceramide, suggesting that association with lipid rafts may influence oligomerization. Surprisingly, monomeric Pma1p present in ceramide-deficient membranes can be exported from the ER in COPII-vesicles in a reaction that is stimulated by Lst1p. We suggest that Lst1p directly conveys Pma1p into a COPII vesicle and that the larger size of Lst1/Sec24p mixed COPII vesicles is not essential to the packaging of large oligomeric complexes.
J. Biol. Chem, 10.1074/jbc.M200450200
Submitted on January 15, 2002
Revised on April 9, 2002
Accepted on April 10, 2002
Ceramide biosynthesis is required for the formation of oligomeric H+-ATPase, Pma1p, in the yeast endoplasmic reticulum
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Dawson, W. M. Toone, N. Jones, and C. R. M. Wilkinson Loss of Regulators of Vacuolar ATPase Function and Ceramide Synthesis Results in Multidrug Sensitivity in Schizosaccharomyces pombe Eukaryot. Cell, June 1, 2008; 7(6): 926 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Ronchi, S. Colombo, M. Francolini, and N. Borgese Transmembrane domain-dependent partitioning of membrane proteins within the endoplasmic reticulum J. Cell Biol., April 3, 2008; 181(1): 105 - 118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Pasrija, S. L. Panwar, and R. Prasad Multidrug Transporters CaCdr1p and CaMdr1p of Candida albicans Display Different Lipid Specificities: both Ergosterol and Sphingolipids Are Essential for Targeting of CaCdr1p to Membrane Rafts Antimicrob. Agents Chemother., February 1, 2008; 52(2): 694 - 704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pagant, L. Kung, M. Dorrington, M. C.S. Lee, and E. A. Miller Inhibiting Endoplasmic Reticulum (ER)-associated Degradation of Misfolded Yor1p Does Not Permit ER Export Despite the Presence of a Diacidic Sorting Signal Mol. Biol. Cell, September 1, 2007; 18(9): 3398 - 3413. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Daquinag, M. Fadri, S. Y. Jung, J. Qin, and J. Kunz The Yeast PH Domain Proteins Slm1 and Slm2 Are Targets of Sphingolipid Signaling during the Response to Heat Stress Mol. Cell. Biol., January 15, 2007; 27(2): 633 - 650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Laloi, A.-M. Perret, L. Chatre, S. Melser, C. Cantrel, M.-N. Vaultier, A. Zachowski, K. Bathany, J.-M. Schmitter, M. Vallet, et al. Insights into the Role of Specific Lipids in the Formation and Delivery of Lipid Microdomains to the Plasma Membrane of Plant Cells Plant Physiology, January 1, 2007; 143(1): 461 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Gaigg, A. Toulmay, and R. Schneiter Very Long-chain Fatty Acid-containing Lipids rather than Sphingolipids per se Are Required for Raft Association and Stable Surface Transport of Newly Synthesized Plasma Membrane ATPase in Yeast J. Biol. Chem., November 10, 2006; 281(45): 34135 - 34145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, S. Sitaraman, and A. Chang Multiple Degradation Pathways for Misfolded Mutants of the Yeast Plasma Membrane ATPase, PMA1 J. Biol. Chem., October 20, 2006; 281(42): 31457 - 31466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Grossmann, M. Opekarova, L. Novakova, J. Stolz, and W. Tanner Lipid Raft-Based Membrane Compartmentation of a Plant Transport Protein Expressed in Saccharomyces cerevisiae Eukaryot. Cell, June 1, 2006; 5(6): 945 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Liu and A. Chang Quality control of a mutant plasma membrane ATPase: ubiquitylation prevents cell-surface stability J. Cell Sci., January 15, 2006; 119(2): 360 - 369. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Proszynski, R. W. Klemm, M. Gravert, P. P. Hsu, Y. Gloor, J. Wagner, K. Kozak, H. Grabner, K. Walzer, M. Bagnat, et al. A genome-wide visual screen reveals a role for sphingolipids and ergosterol in cell surface delivery in yeast PNAS, December 13, 2005; 102(50): 17981 - 17986. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Zaremberg, C. Gajate, L. M. Cacharro, F. Mollinedo, and C. R. McMaster Cytotoxicity of an Anti-cancer Lysophospholipid through Selective Modification of Lipid Raft Composition J. Biol. Chem., November 11, 2005; 280(45): 38047 - 38058. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Gaigg, B. Timischl, L. Corbino, and R. Schneiter Synthesis of Sphingolipids with Very Long Chain Fatty Acids but Not Ergosterol Is Required for Routing of Newly Synthesized Plasma Membrane ATPase to the Cell Surface of Yeast J. Biol. Chem., June 10, 2005; 280(23): 22515 - 22522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Zink, C. Mehlgarten, H. K. Kitamoto, J. Nagase, D. Jablonowski, R. C. Dickson, M. J. R. Stark, and R. Schaffrath Mannosyl-Diinositolphospho-Ceramide, the Major Yeast Plasma Membrane Sphingolipid, Governs Toxicity of Kluyveromyces lactis Zymocin Eukaryot. Cell, May 1, 2005; 4(5): 879 - 889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Vandenberghe, R. A. Nicoll, and D. S. Bredt Stargazin is an AMPA receptor auxiliary subunit PNAS, January 11, 2005; 102(2): 485 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Malinska, J. Malinsky, M. Opekarova, and W. Tanner Distribution of Can1p into stable domains reflects lateral protein segregation within the plasma membrane of living S. cerevisiae cells J. Cell Sci., December 1, 2004; 117(25): 6031 - 6041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Paladino, D. Sarnataro, R. Pillich, S. Tivodar, L. Nitsch, and C. Zurzolo Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins J. Cell Biol., November 22, 2004; 167(4): 699 - 709. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Lefebvre, H. Batoko, G. Duby, and M. Boutry Targeting of a Nicotiana plumbaginifolia H+-ATPase to the Plasma Membrane Is Not by Default and Requires Cytosolic Structural Determinants PLANT CELL, July 1, 2004; 16(7): 1772 - 1789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pizzirusso and A. Chang Ubiquitin-mediated Targeting of a Mutant Plasma Membrane ATPase, Pma1-7, to the Endosomal/Vacuolar System in Yeast Mol. Biol. Cell, May 1, 2004; 15(5): 2401 - 2409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. J. Proszynski, K. Simons, and M. Bagnat O-Glycosylation as a Sorting Determinant for Cell Surface Delivery in Yeast Mol. Biol. Cell, April 1, 2004; 15(4): 1533 - 1543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Malinska, J. Malinsky, M. Opekarova, and W. Tanner Visualization of Protein Compartmentation within the Plasma Membrane of Living Yeast Cells Mol. Biol. Cell, November 1, 2003; 14(11): 4427 - 4436. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sato and A. Nakano Oligomerization of a Cargo Receptor Directs Protein Sorting into COPII-coated Transport Vesicles Mol. Biol. Cell, July 1, 2003; 14(7): 3055 - 3063. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Hearn, R. L. Lester, and R. C. Dickson The Uracil Transporter Fur4p Associates with Lipid Rafts J. Biol. Chem., January 31, 2003; 278(6): 3679 - 3686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Eisenkolb, C. Zenzmaier, E. Leitner, and R. Schneiter A Specific Structural Requirement for Ergosterol in Long-chain Fatty Acid Synthesis Mutants Important for Maintaining Raft Domains in Yeast Mol. Biol. Cell, December 1, 2002; 13(12): 4414 - 4428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Wang and A. Chang Sphingoid base synthesis is required for oligomerization and cell surface stability of the yeast plasma membrane ATPase, Pma1 PNAS, October 1, 2002; 99(20): 12853 - 12858. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
