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A more recent version of this article appeared on May 24, 2002
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M200500200v1
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Papers In Press, published online ahead of print March 23, 2002
J. Biol. Chem, 10.1074/jbc.M200500200
Submitted on January 16, 2002
Revised on March 20, 2002
Accepted on March 22, 2002

A common mechanism of stage-regulated gene expression in Leishmania mediated by a conserved 3' UTR element

Nathalie Boucher, Ying Wu, Carole Dumas, Marthe Dubé, Denis Sereno, Marie Breton, and Barbara Papadopoulou

Deparment of Medical Biology, Laval University, Ste-Foy, Québec G1V 4G2

Corresponding Author: barbara.papadopoulou{at}crchul.ulaval.ca

Developmental regulation of mRNA levels in trypanosomatid protozoa is determined post-transcriptionally and often involves sequences located in the 3’untranslated regions (3’UTR) of the mRNAs. We have previously identified a developmentally regulated gene family in Leishmania encoding the amastin surface proteins and showed that stage-specific accumulation of the amastin mRNA is mediated by sequences within the 3’UTR. Here we identified a 450 nt region within the amastin 3’UTR, which can confer amastigote-specific gene expression by a novel mechanism that increases mRNA translation without an increase in mRNA stability. Remarkably, this 450 nt 3’UTR element is highly conserved among a large number of Leishmania mRNAs in several Leishmania species. Here we show that several of these mRNAs are differentially expressed in the intracellular amastigote stage of the parasite and that the 450 nt conserved element in their 3’UTRs is responsible for stage-specific gene regulation. We propose that the 450 nt conserved element, which is unlike any other regulatory element identified thus far, is part of a common mechanism of stage-regulated gene expression in Leishmania that regulates mRNA translation in response to intracellular stresses.


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