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Papers In Press, published online ahead of print July 19, 2002
Division of Biological Sciences, Osaka University, Ibaraki, Osaka 567-0047
Corresponding Author: m-futai{at}sanken.osaka-u.ac.jp
Vacuolar-type H+-ATPases (V-ATPases), a family of multimeric proton pumps, are involved in a wide variety of physiological processes. We have identified two mouse genes, Atp6g1 and Atp6g2, encoding isoforms (G1 and G2, respectively) of V-ATPase subunit G. G1 was distributed ubiquitously in the tissues examined, whereas G2 was specifically distributed in central nervous system neurons. G1 was expressed at an early embryonic stage, whereas G2 transcription was significantly induced at 10.5 dpc (embryonic day 10.5, i.e. 2 days before axon outgrowth). Both G1 and G2 were strongly expressed in cortical and hippocampal neurons, cerebellar granule cells and Purkinje cells. Immunohistochemistry with isoform-specific antibodies revealed that G2 was localized in the cell bodies, dendrites, and axons. In addition, electron microscopy and subcellular fractionation indicated that the G2 was localized in synaptic vesicles where G1 was not detectable. G1 and G2 exhibit 62% identity, and both isoform were immunoprecipitated with the c and A subunits of V-ATPase. G2 could complement the yeast deletion mutant (delta vma10) lacking the G subunit. The V-ATPase containing G1 or G2 isoform showed similar KmATP and maximal velocity. These results indicate that G1 and G2 are bona fide subunits of V-ATPases, and that the enzyme with G2 isoform is involved in synaptic vesicle acidification.
J. Biol. Chem, 10.1074/jbc.M200586200
Submitted on January 18, 2002
Revised on July 15, 2002
Accepted on July 18, 2002
Differential localization of V-type H+ pump with G subunit isoforms (G1 and G2) in mouse neurons
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