| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Papers In Press, published online ahead of print March 21, 2002
J. Biol. Chem, 10.1074/jbc.M201878200
Submitted on February 25, 2002
Revised on March 21, 2002
Accepted on March 21, 2002
Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210
Corresponding Author: herman.81{at}osu.edu
The S. cerevisiae Ras proteins control cell growth by regulating the activity of the cAMP-dependent protein kinase, PKA. In this study, a genetic approach was used to identify cellular processes that were regulated by Ras/PKA signaling activity. Interestingly, we found that mutations affecting the C-terminal domain, or CTD, of Rpb1p, the largest subunit of RNA polymerase II, were very sensitive to changes in Ras signaling activity. The Rpb1p CTD is a highly conserved, repetitive structure that is a key site of control during the production of a mature mRNA molecule. We found that mutations compromising the CTD were synthetically lethal with alterations that led to elevated levels of Ras/PKA signaling. Altogether, the data suggested that Ras/PKA activity was negatively regulating a protein that functioned in concert with the CTD during RNA pol II transcription. Consistent with this prediction, we found that elevated levels of Ras signaling caused growth and transcription defects that were very similar to those observed in mutants encoding an Rpb1p with a truncated CTD. In all, these data suggested that S. cerevisiae growth control and RNA pol II transcription might be coupled by using the Ras pathway to regulate CTD function.
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  S. J. Deminoff, S. C. Howard, A. Hester, S. Warner, and P. K. Herman Using Substrate-Binding Variants of the cAMP-Dependent Protein Kinase to Identify Novel Targets and a Kinase Domain Important for Substrate Interactions in Saccharomyces cerevisiae Genetics, August 1, 2006; 173(4): 1909 - 1917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. M. Santangelo Glucose Signaling in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., March 1, 2006; 70(1): 253 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. V. Budovskaya, J. S. Stephan, F. Reggiori, D. J. Klionsky, and P. K. Herman The Ras/cAMP-dependent Protein Kinase Signaling Pathway Regulates an Early Step of the Autophagy Process in Saccharomyces cerevisiae J. Biol. Chem., May 14, 2004; 279(20): 20663 - 20671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Howard, A. Hester, and P. K. Herman The Ras/PKA Signaling Pathway May Control RNA Polymerase II Elongation via the Spt4p/Spt5p Complex in Saccharomyces cerevisiae Genetics, November 1, 2003; 165(3): 1059 - 1070. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| All ASBMB Journals | Molecular and Cellular Proteomics |
| Journal of Lipid Research | ASBMB Today |
