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Papers In Press, published online ahead of print May 13, 2002
Cystic Fibrosis Research Laboratory, Stanford University, Stanford, CA 94305-2130
Corresponding Author: wine{at}stanford.edu
Secretion rates of > 700 individual glands in isolated tracheal mucosa from 56 adult pigs were monitored optically. 'Basal' secretion of 0.7 ± 0.1 nl/min/gland was observed 1-9 hr post-harvest, but was near zero on day 2. Secretion to carbachol (10
J. Biol. Chem, 10.1074/jbc.M202712200
Submitted on March 20, 2002
Revised on April 29, 2002
Accepted on May 13, 2002
Mucus secretion from single submucosal glands of pig: Stimulation by carbachol and vasoactive intestinal peptide
M) peaked at 2-3 min and then declined to a sustained phase. Peak secretion was 12.5 ± 1.1 nl/min/gl; sustained secretion was ~ 1/3 of peak secretion. Thapsigargin (1 M) increased secretion from 0.1 ± 0.05 to 0.7 ± 0.2 nl/min/gl; thapsigargin did not cause contraction of the trachealis muscles. Isoproterenol and phenylephrine (10 M each) were ineffective, but vasoactive intestinal peptide (1 M) and forskolin (10 M) each produced sustained secretion of 1.0 ± 0.5 and 1.7 ± 0.2 nl/min/gl respectively. The density of actively secreting glands was 1.3/mm2. Secretion to either carbachol or forskolin was inhibited (~50%) by either bumetanide or bicarbonate removal, and inhibited ~90% by the combined treatments. Mucus secreted in response to carbachol or forskolin was acidic by ~0.2 pH unit relative to the bath and remained acidic by ~0.1 pH unit after bumetanide. The strong secretory response to VIP, the acidity of [cAMP]i -stimulated mucus and its inhibition by bumetanide were unexpected.
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