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Papers In Press, published online ahead of print May 13, 2002
J. Biol. Chem, 10.1074/jbc.M203205200
Submitted on April 4, 2002
Revised on May 13, 2002
Accepted on May 10, 2002
Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow G11 6NU
Corresponding Author: gbga05{at}udcf.gla.ac.uk
We demonstrate, by gene deletion analysis, that Mre11 has a critical role in maintaining genomic integrity in Trypanosoma brucei. mre11-/- null mutant strains exhibited retarded growth, but no delay or disruption of cell cycle progression. They showed also a weak hyporecombination phenotype and the accumulation of gross chromosomal rearrangements, which did not involve sequence translocation, telomere loss or formation of new telomeres. The trypanosome mre11-/- strains were hypersensitive to phleomycin, a mutagen causing DNA double strand breaks (DSBs) but, in contrast to mre11-/- null mutants in other organisms and T. brucei rad51-/- null mutants, displayed no hypersensitivity to methylmethane sulphonate (MMS), which causes point mutations and DSBs. Mre11 therefore is important for the repair of chromosomal damage and DSBs in trypanosomes, although in this organism the intersection of repair pathways appears to differ from that in other organisms. Mre11 inactivation appears not to affect VSG gene switching during antigenic variation of a laboratory strain, which is perhaps surprising given the importance of homologous recombination during this process.
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