JBC

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on August 9, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/33/29753    most recent
M203728200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vega-Agapito, V.
Right arrow Articles by Bolaños, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vega-Agapito, V.
Right arrow Articles by Bolaños, J. P.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print June 10, 2002
J. Biol. Chem, 10.1074/jbc.M203728200
Submitted on April 17, 2002
Revised on June 5, 2002
Accepted on June 10, 2002

Peroxynitrite stimulates L-arginine transport system y+ in glial cells: A potential mechanism for replenishing neuronal L-arginine

Victoria Vega-Agapito, Angeles Almeida, Maria Hatzoglou, and Juan P. Bolaños

Bioquimica y Biologia Molecular, Universidad de Salamanca, Salamanca 37007

Corresponding Author: jbolanos{at}gugu.usal.es

We have previously reported that peroxynitrite stimulates L-arginine release from astrocytes, but the mechanism responsible for such an effect remains elusive. To explore this issue, we studied the regulation of L-[3H]arginine transport by either exogenous or endogenous peroxynitrite in glial cells. A twofold peroxynitrite-mediated stimulation of L-arginine release in C6 cells was found to be sodium-independent, was prevented by 5 mM L-arginine and, although only in the presence of sodium, was blocked by 5 mM L-alanine or L-leucine. Peroxynitrite-mediated stimulation of L-arginine uptake was trans-stimulated by 10 mM L-arginine and was inhibited in a dose-dependent fashion (ki of aprox. 40 micromolar) by the system y+ inhibitor N-ethylmaleimide in C6 cells. Endogenous production of peroxynitrite in lipopolysaccharide-treated astrocytes triggered an increased L-arginine transport activity without affecting Cat1 L-arginine transporter mRNA levels. However, western blot analyses of peroxynitrite-treated astrocytes and C6 glial cells revealed a 3-nitrotyrosinated anti-Cat1-immunopositive band, strongly suggesting peroxynitrite-mediated Cat1 nitration. Furthermore, peroxynitrite stimulation of L-arginine release was abolished in fibroblast cells homozygous for a targetted inactivation of the Cat1 gene. Finally, peroxynitrite-triggered L-arginine released from astrocytes was efficiently uptaken by neurons in an insert-based co-culture system. These results strongly suggest that peroxynitrite-mediated activation of Cat1 transporter in glial cells may serve as a mechanism focused to replenish L-arginine in the neighboring neurons.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nephrol Dial TransplantHome page
I. F. Schwartz, T. Chernichovsky, D. Hagin, M. Ingbir, R. Reshef, G. Chernin, Y. Levo, and D. Schwartz
Differential regulation of L-arginine transporters (cationic amino acid transporter-1 and -2) by peroxynitrite in rat mesangial cells
Nephrol. Dial. Transplant., December 1, 2006; 21(12): 3409 - 3414.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. L. O'Kane, J. R. Vina, I. Simpson, R. Zaragoza, A. Mokashi, and R. A. Hawkins
Cationic amino acid transport across the blood-brain barrier is mediated exclusively by system y+
Am J Physiol Endocrinol Metab, August 1, 2006; 291(2): E412 - E419.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Garcia-Nogales, A. Almeida, and J. P. Bolanos
Peroxynitrite Protects Neurons against Nitric Oxide-mediated Apoptosis. A KEY ROLE FOR GLUCOSE-6-PHOSPHATE DEHYDROGENASE ACTIVITY IN NEUROPROTECTION
J. Biol. Chem., January 3, 2003; 278(2): 864 - 874.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.