JBC Connect with Cosmo for Collagen Detection

HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

A more recent version of this article appeared on October 11, 2002
This Article
Right arrow Full Text (Accepted Manuscript)
Right arrow All Versions of this Article:
277/42/39217    most recent
M205209200v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vetcher, A. A.
Right arrow Articles by Wells, R. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vetcher, A. A.
Right arrow Articles by Wells, R. D.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Papers In Press, published online ahead of print August 2, 2002
J. Biol. Chem, 10.1074/jbc.M205209200
Submitted on May 28, 2002
Revised on July 30, 2002
Accepted on August 1, 2002

Sticky DNA, a long GAA5GAA5TTC triplex which is formed intramolecularly, in the sequence of intron 1 of the Frataxin gene

Alexandre A. Vetcher, Marek Napierala, Ravi R. Iyer, Paul D. Chastain, Jack D. Griffith, and Robert D. Wells

Center for Genome Research, Institute of Biosciences & Technology, Houston, TX 77030

Corresponding Author: rwells{at}ibt.tamu.edu

Friedreich’s ataxia (FRDA) is caused by the massive expansion of GAA•TTC repeats in intron 1 of the frataxin (X25) gene. Our prior investigations showed that long GAA•TTC repeats formed very stable triplex structures which caused two repeat tracts to adhere to each other (sticky DNA). This process was dependent on negative supercoiling and the presence of divalent metal ions. Herein, we have investigated the formation of sticky DNA from plasmid monomers and dimers; sticky DNA is formed only when two tracts of sufficiently long (GAA•TTC)n (n = 59 – 270) are present in a single plasmid DNA and are in the direct repeat orientation. If the inserts are in the indirect (inverted) repeat orientation, no sticky DNA was observed. Furthermore, kinetic studies support the intramolecular nature of sticky DNA formation. Electron microscopy (EM) investigations also provide strong data for sticky DNA as a single long triplex. Hence, these results give new insights into our understanding of the capacity of sticky DNA to inhibit transcription and thereby reduce the level of frataxin protein as related to the etiology of FRDA.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
E. Soragni, D. Herman, S. Y. R. Dent, J. M. Gottesfeld, R. D. Wells, and M. Napierala
Long intronic GAA*TTC repeats induce epigenetic changes and reporter gene silencing in a molecular model of Friedreich ataxia
Nucleic Acids Res., September 27, 2008; (2008) gkn604v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
L. M. Pollard, Y. K. Chutake, P. M. Rindler, and S. I. Bidichandani
Deficiency of RecA-dependent RecFOR and RecBCD pathways causes increased instability of the (GAA{middle dot}TTC)n sequence when GAA is the lagging strand template
Nucleic Acids Res., November 29, 2007; 35(20): 6884 - 6894.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
E. V. Mirkin and S. M. Mirkin
Replication Fork Stalling at Natural Impediments
Microbiol. Mol. Biol. Rev., March 1, 2007; 71(1): 13 - 35.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. M. Krasilnikova, M. L. Kireeva, V. Petrovic, N. Knijnikova, M. Kashlev, and S. M. Mirkin
Effects of Friedreich's ataxia (GAA)n{middle dot}(TTC)n repeats on RNA synthesis and stability
Nucleic Acids Res., February 28, 2007; 35(4): 1075 - 1084.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Wojciechowska, M. Napierala, J. E. Larson, and R. D. Wells
Non-B DNA Conformations Formed by Long Repeating Tracts of Myotonic Dystrophy Type 1, Myotonic Dystrophy Type 2, and Friedreich's Ataxia Genes, Not the Sequences per se, Promote Mutagenesis in Flanking Regions
J. Biol. Chem., August 25, 2006; 281(34): 24531 - 24543.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Burnett, C. Melander, J. W. Puckett, L. S. Son, R. D. Wells, P. B. Dervan, and J. M. Gottesfeld
DNA sequence-specific polyamides alleviate transcription inhibition associated with long GAA{middle dot}TTC repeats in Friedreich's ataxia
PNAS, August 1, 2006; 103(31): 11497 - 11502.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Bacolla, J. R. Collins, B. Gold, N. Chuzhanova, M. Yi, R. M. Stephens, S. Stefanov, A. Olsh, J. P. Jakupciak, M. Dean, et al.
Long homopurine*homopyrimidine sequences are characteristic of genes expressed in brain and the pseudoautosomal region.
Nucleic Acids Res., January 1, 2006; 34(9): 2663 - 2675.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Napierala, A. Bacolla, and R. D. Wells
Increased Negative Superhelical Density in Vivo Enhances the Genetic Instability of Triplet Repeat Sequences
J. Biol. Chem., November 11, 2005; 280(45): 37366 - 37376.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. D. Wells, R. Dere, M. L. Hebert, M. Napierala, and L. S. Son
Advances in mechanisms of genetic instability related to hereditary neurological diseases
Nucleic Acids Res., July 8, 2005; 33(12): 3785 - 3798.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Wojciechowska, A. Bacolla, J. E. Larson, and R. D. Wells
The Myotonic Dystrophy Type 1 Triplet Repeat Sequence Induces Gross Deletions and Inversions
J. Biol. Chem., January 14, 2005; 280(2): 941 - 952.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Bacolla and R. D. Wells
Non-B DNA Conformations, Genomic Rearrangements, and Human Disease
J. Biol. Chem., November 12, 2004; 279(46): 47411 - 47414.
[Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. M. Krasilnikova and S. M. Mirkin
Replication Stalling at Friedreich's Ataxia (GAA)n Repeats In Vivo
Mol. Cell. Biol., March 15, 2004; 24(6): 2286 - 2295.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y.-L. Kang, H. Li, W.-H. Chen, Y.-S. Tzeng, Y.-L. Lai, and H. M. Hsieh-Li
A Novel PEPP Homeobox Gene, TOX, Is Highly Glutamic Acid Rich and Specifically Expressed in Murine Testis and Ovary
Biol Reprod, March 1, 2004; 70(3): 828 - 836.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. N. Potaman, E. A. Oussatcheva, Y. L. Lyubchenko, L. S. Shlyakhtenko, S. I. Bidichandani, T. Ashizawa, and R. R. Sinden
Length-dependent structure formation in Friedreich ataxia (GAA)n{middle dot}(TTC)n repeats at neutral pH
Nucleic Acids Res., February 20, 2004; 32(3): 1224 - 1231.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. A. Vetcher and R. D. Wells
Sticky DNA Formation in Vivo Alters the Plasmid Dimer/Monomer Ratio
J. Biol. Chem., February 20, 2004; 279(8): 6434 - 6443.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Napierala, R. Dere, A. Vetcher, and R. D. Wells
Structure-dependent Recombination Hot Spot Activity of GAA{middle dot}TTC Sequences from Intron 1 of the Friedreich's Ataxia Gene
J. Biol. Chem., February 20, 2004; 279(8): 6444 - 6454.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. A. Vetcher, M. Napierala, and R. D. Wells
Sticky DNA: Effect of the Polypurine{middle dot}Polypyrimidine Sequence
J. Biol. Chem., October 11, 2002; 277(42): 39228 - 39234.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 All ASBMB Journals   Molecular and Cellular Proteomics 
 Journal of Lipid Research   ASBMB Today 
Copyright © 2002 by the American Society for Biochemistry and Molecular Biology.