Identification and characterization of a soluble cadherin-7 isoform produced by alternative splicing

doi:10.1074/jbc.M205328200

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Papers In Press, published online ahead of print October 2, 2002
J. Biol. Chem, 10.1074/jbc.M205328200
Submitted on May 30, 2002
Revised on September 24, 2002
Accepted on September 27, 2002

Identification and characterization of a soluble cadherin-7 isoform produced by alternative splicing

Rie Kawano, Noritaka Matsuo, Hideaki Tanaka, Masaru Nasu, Hidekatsu Yoshioka, and Komei Shirabe

Anatomy, Biology and Medicine, Oita Medical University, Hasama-machi, Oita 879-5593

Corresponding Author: riekawa{at}oita-med.ac.jp

We identified an alternative mRNA encoding a novel cadherin-7 isoform by reverse transcriptase-polymerase chain reaction (RT-PCR) of RNA from day 12 chicken embryos. The alternative mRNA contains 49 bases of insertion in the premembrane region, leading to the substitution of 14 amino acids and the introduction of a premature stop codon. Identification of a 49 bp insertion sequence in the genomic DNA corresponding to the intron of the cadherin-7 gene, suggests that alternative splicing is the cause of the alternative mRNA. Transient expression of the variant form in Cos-7 or 293 cells produced a soluble protein. Aggregation assays and immunoprecipitation showed that the variant protein interacts with full-length cadherin-7 in vitro and in vivo and inhibits full-length cadherin-7-mediated cell adhesion. Immunohistochemistry revealed that the variant form was strongly expressed in dermomyotomes rather than in migrating neural crest cells, in contrast to the full-length cadherin-7, suggesting differential regulation of splicing and possible roles of variant cadherin-7 in the development of dermomyotomes and other tissues.

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