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A more recent version of this article appeared on October 11, 2002
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Papers In Press, published online ahead of print August 12, 2002
J. Biol. Chem, 10.1074/jbc.M205535200
Submitted on June 4, 2002
Revised on August 12, 2002
Accepted on August 12, 2002

CIN85 participates in Cbl-b-mediated downregulation of receptor tyrosine kinases

Iwona Szymkiewicz, Katarzyna Kowanetz, Philippe Soubeyran, Ana Dinarina, Stanley Lipkowitz, and Ivan Dikic

Ludwig Institute for Cancer Research, Uppsala SE-75124

Corresponding Author: Ivan.Dikic{at}licr.uu.se

The Cbl family of ubiquitin ligases in mammals contains three members Cbl, Cbl-b and Cbl-3 involved in downregulation of receptor tyrosine kinases (RTKs) by mediating receptor ubiquitination and degradation. More recently, a novel pathway has been identified whereby Cbl promotes internalization of EGF receptor via a CIN85/endophilin pathway that is functionally separable from the ubiquitin ligase activity of Cbl (1). Here we show that Cbl-b, but not Cbl-3, utilize the same mechanism to downregulate multiple RTKs. CIN85 was shown to bind to the minimal binding domain identified in the carboxyl terminus of Cbl-b. Ligand-induced phosphorylation of Cbl-b further increased their interactions and led to a rapid and sustained recruitment of CIN85 in the complex with EGF or PDGF receptors. Inhibition of binding between CIN85 and Cbl-b was sufficient to impair Cbl-b-mediated internalization of EGF receptors, while being dispensable for Cbl-b-directed polyubiquitination of EGF receptors. Moreover, CIN85 and Cbl/Cbl-b were constitutively associated with activated PDGF, EGF or c-Kit receptors in several tumor cell lines. Our data reveal a common pathway utilized by Cbl and Cbl-b that may have an important and redundant function in negative regulation of ligand- as well as oncogenically-activated RTKs in vivo.


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